2022
An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination
Kanca O, Zirin J, Hu Y, Tepe B, Dutta D, Lin W, Ma L, Ge M, Zuo Z, Liu L, Levis R, Perrimon N, Bellen H. An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination. ELife 2022, 11: e76077. PMID: 35723254, PMCID: PMC9239680, DOI: 10.7554/elife.76077.Peer-Reviewed Original Research
2016
Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster
Mavor L, Miao H, Zuo Z, Holly R, Xie Y, Loerke D, Blankenship J. Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster. Development 2016, 143: 892-903. PMID: 26839362, PMCID: PMC4813336, DOI: 10.1242/dev.128876.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAnimals, Genetically ModifiedCell MembraneCRISPR-Cas SystemsCrosses, GeneticCytoplasmDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianEpitheliumExocytosisFemaleGene Expression Regulation, DevelopmentalGolgi ApparatusGTP PhosphohydrolasesGuanosine TriphosphateMaleMembrane ProteinsMicroscopy, ConfocalProtein Structure, TertiaryRab GTP-Binding ProteinsConceptsFurrow ingressionMembrane additionPlasma membranePlasma membrane furrowsLarge cytoplasmic aggregatesCRISPR/Cas9 technologyIntracellular trafficking pathwaysMembrane furrowsRab8 functionDrosophila embryosDrosophila melanogasterTrafficking pathwaysMembrane compartmentsEndogenous localizationProtein Rab11Early embryosCytoplasmic aggregatesCas9 technologyRab8Membrane storesCell surfaceEpithelial sheetsRab11Cell morphologyCompartmental behavior