2022
An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination
Kanca O, Zirin J, Hu Y, Tepe B, Dutta D, Lin W, Ma L, Ge M, Zuo Z, Liu L, Levis R, Perrimon N, Bellen H. An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination. ELife 2022, 11: e76077. PMID: 35723254, PMCID: PMC9239680, DOI: 10.7554/elife.76077.Peer-Reviewed Original ResearchAnimalsClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsDrosophilaExonsHomologous RecombinationPlasmids
2019
An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
Kanca O, Zirin J, Garcia-Marques J, Knight S, Yang-Zhou D, Amador G, Chung H, Zuo Z, Ma L, He Y, Lin W, Fang Y, Ge M, Yamamoto S, Schulze K, Hu Y, Spradling A, Mohr S, Perrimon N, Bellen H. An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. ELife 2019, 8: e51539. PMID: 31674908, PMCID: PMC6855806, DOI: 10.7554/elife.51539.Peer-Reviewed Original ResearchAnimalsClustered Regularly Interspaced Short Palindromic RepeatsDNADNA, Single-StrandedDrosophilaGene Knock-In TechniquesHomologous RecombinationMutagenesis, Insertional
2018
An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila
Li-Kroeger D, Kanca O, Lee P, Cowan S, Lee M, Jaiswal M, Salazar J, He Y, Zuo Z, Bellen H. An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. ELife 2018, 7: e38709. PMID: 30091705, PMCID: PMC6095692, DOI: 10.7554/elife.38709.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsClustered Regularly Interspaced Short Palindromic RepeatsDrosophilaEntomologyGene TargetingGenes, ReporterIntegrasesMutagenesis, InsertionalConceptsNew genetic toolsDouble headerTagged alleleGene taggingPrecise gene manipulationEndogenous genesGenetic toolsProtein functionProtein localizationMultiple downstream applicationsArtificial exonCassette exchangeProtein trappingEmbryo injectionNull allelesExpression patternsGene manipulationGene expressionDNA fragmentsDominant markersGenesCRISPRIntegration efficiencyDownstream applicationsAlleles