2017
The Krebs Cycle Enzyme Isocitrate Dehydrogenase 3A Couples Mitochondrial Metabolism to Synaptic Transmission
Ugur B, Bao H, Stawarski M, Duraine LR, Zuo Z, Lin YQ, Neely GG, Macleod GT, Chapman ER, Bellen HJ. The Krebs Cycle Enzyme Isocitrate Dehydrogenase 3A Couples Mitochondrial Metabolism to Synaptic Transmission. Cell Reports 2017, 21: 3794-3806. PMID: 29281828, PMCID: PMC5747319, DOI: 10.1016/j.celrep.2017.12.005.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesKrebs cycle enzymeRole of metabolitesC2 domainPlasma membraneMitochondrial metabolismSynaptic transmissionMetabolic regulationCycle enzymesSynaptic roleAlpha-ketoglutarateSyt1ΑKGNeurodegenerative disordersDependent processesRegulationMetabolitesIDH3ASynaptotagmin1Multiple levelsFliesRoleFusionVesiclesATP
2016
Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster
Mavor L, Miao H, Zuo Z, Holly R, Xie Y, Loerke D, Blankenship J. Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster. Development 2016, 143: 892-903. PMID: 26839362, PMCID: PMC4813336, DOI: 10.1242/dev.128876.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAnimals, Genetically ModifiedCell MembraneCRISPR-Cas SystemsCrosses, GeneticCytoplasmDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianEpitheliumExocytosisFemaleGene Expression Regulation, DevelopmentalGolgi ApparatusGTP PhosphohydrolasesGuanosine TriphosphateMaleMembrane ProteinsMicroscopy, ConfocalProtein Structure, TertiaryRab GTP-Binding ProteinsConceptsFurrow ingressionMembrane additionPlasma membranePlasma membrane furrowsLarge cytoplasmic aggregatesCRISPR/Cas9 technologyIntracellular trafficking pathwaysMembrane furrowsRab8 functionDrosophila embryosDrosophila melanogasterTrafficking pathwaysMembrane compartmentsEndogenous localizationProtein Rab11Early embryosCytoplasmic aggregatesCas9 technologyRab8Membrane storesCell surfaceEpithelial sheetsRab11Cell morphologyCompartmental behavior
2015
A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryo
Holly R, Mavor L, Zuo Z, Blankenship J. A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryo. Development 2015, 142: 2316-2328. PMID: 26092850, PMCID: PMC4510590, DOI: 10.1242/dev.120998.Peer-Reviewed Original ResearchConceptsEarly Drosophila embryoDrosophila embryosFurrow formationPlasma membraneRalA functionMembrane trafficking pathwaysEarly syncytial Drosophila embryoExocyst complex subunitsEarly fly embryoSyncytial Drosophila embryosWild-type embryosMyosin II functionF-actin recruitmentExocyst complexExocytic vesiclesMembrane traffickingFly embryoTrafficking pathwaysComplex subunitsFurrow ingressionChromosomal segregationCytoskeletal remodelingCell divisionCytokinetic furrowRalA