2016
Discovery of a Coregulatory Interaction between Kaposi's Sarcoma-Associated Herpesvirus ORF45 and the Viral Protein Kinase ORF36
Avey D, Tepper S, Pifer B, Bahga A, Williams H, Gillen J, Li W, Ogden S, Zhu F. Discovery of a Coregulatory Interaction between Kaposi's Sarcoma-Associated Herpesvirus ORF45 and the Viral Protein Kinase ORF36. Journal Of Virology 2016, 90: 5953-5964. PMID: 27099309, PMCID: PMC4907238, DOI: 10.1128/jvi.00516-16.Peer-Reviewed Original ResearchConceptsKaposi's sarcoma-associated herpesvirusKSHV ORF36Viral protein kinaseSarcoma-associated herpesvirusProtein kinaseKSHV lytic replicationKaposi's Sarcoma-Associated Herpesvirus ORF45Infectious progeny virionsHerpesvirus protein kinasesProgeny virionsLytic replicationKinase-dead mutantBacterial artificial chromosome mutagenesisBasic N-terminusCoregulatory interactionsChromosome mutagenesisORF45 proteinViral life cycleAcidic patchKSHV proteinsORF36Human tumor virusKinase activityORF45N-terminus
2014
Activation of p90 Ribosomal S6 Kinases by ORF45 of Kaposi's Sarcoma-Associated Herpesvirus Is Critical for Optimal Production of Infectious Viruses
Fu B, Kuang E, Li W, Avey D, Li X, Turpin Z, Valdes A, Brulois K, Myoung J, Zhu F. Activation of p90 Ribosomal S6 Kinases by ORF45 of Kaposi's Sarcoma-Associated Herpesvirus Is Critical for Optimal Production of Infectious Viruses. Journal Of Virology 2014, 89: 195-207. PMID: 25320298, PMCID: PMC4301102, DOI: 10.1128/jvi.01937-14.Peer-Reviewed Original ResearchConceptsRibosomal S6 kinaseP90 ribosomal S6 kinaseKaposi's sarcoma-associated herpesvirusViral gene expressionKSHV lytic replicationSarcoma-associated herpesvirusRSK activationS6 kinaseGene expressionVirus-infected cellsLytic reactivationWild-type virus-infected cellsMutant virus-infected cellsBacterial artificial chromosomeAlanine-scanning mutagenesisLytic replicationCritical rolePhosphoproteomic profilesArtificial chromosomesKinase ERKScanning mutagenesisProgeny viral particlesKSHV genomeInfectious progeny virionsORF45