2023
Bacteria require phase separation for fitness in the mammalian gut
Krypotou E, Townsend G, Gao X, Tachiyama S, Liu J, Pokorzynski N, Goodman A, Groisman E. Bacteria require phase separation for fitness in the mammalian gut. Science 2023, 379: 1149-1156. PMID: 36927025, PMCID: PMC10148683, DOI: 10.1126/science.abn7229.Peer-Reviewed Original ResearchConceptsMammalian gutTranscription termination factor RhoTermination factor RhoGene regulationTranscription terminationMechanisms bacteriaBacteria interactionsHuman commensalValuable targetBacteriaRhoGut microbiotaFitnessNovel clinical applicationsTherapeutic manipulationGutHuman healthCommensalRegulation
2018
Using disruptive insertional mutagenesis to identify the in situ structure‐function landscape of the Shigella translocator protein IpaB
Barta ML, Tachiyama S, Muthuramalingam M, Arizmendi O, Villanueva CE, Ramyar KX, Geisbrecht BV, Lovell S, Battaile KP, Picking WL, Picking WD. Using disruptive insertional mutagenesis to identify the in situ structure‐function landscape of the Shigella translocator protein IpaB. Protein Science 2018, 27: 1392-1406. PMID: 29672980, PMCID: PMC6153406, DOI: 10.1002/pro.3428.Peer-Reviewed Original ResearchConceptsTip complexInsertional mutagenesisTranslocator protein IpaBBacterial type III secretion systemType III secretion systemC-terminal halfN-terminal halfInsertion mutantsT3SS apparatusCellular functionsLatter mutantSecretion inductionMammalian cellsSecretion systemTC functionHost membraneBasal bodiesBacteriophage T4 lysozymeTertiary structureImpaired interactionIpaBPore formationT4 lysozymeBacterial surfaceMutants