2023
Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure
Barger S, Penfield L, Bahmanyar S. Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure. Journal Of Cell Science 2023, 136: jcs261385. PMID: 37795681, PMCID: PMC10668030, DOI: 10.1242/jcs.261385.Peer-Reviewed Original ResearchConceptsNuclear envelope assemblySpindle microtubulesNE assemblyEnvelope assemblyC. elegans oocytesLEM-2C. elegansHelix domainBAF-1Family proteinsNucleoplasmic poolNE formationDistinct rolesMicrotubulesAdditional roleNE stabilityPermeability barrierRedundant mechanismsBAFProteinEmbryo survivalBindingAssemblyElegansAutointegration
2020
Lipid and protein dynamics that shape nuclear envelope identity
Bahmanyar S, Schlieker C. Lipid and protein dynamics that shape nuclear envelope identity. Molecular Biology Of The Cell 2020, 31: 1315-1323. PMID: 32530796, PMCID: PMC7353140, DOI: 10.1091/mbc.e18-10-0636.Peer-Reviewed Original ResearchConceptsNuclear envelopeEndoplasmic reticulumMembrane fusionNuclear pore complex biogenesisUnique protein compositionBulk endoplasmic reticulumDe novo lipid synthesisNPC biogenesisComplex biogenesisNovo lipid synthesisLipid asymmetryProtein dynamicsProtein compositionElusive mechanismLipid synthesisLipid bilayersBiogenesisPermeability barrierFunctional specializationMajor threatLipid metabolismUnique compositionMitosisReticulumCompartmentalizationRegulated lipid synthesis and LEM2/CHMP7 jointly control nuclear envelope closure
Penfield L, Shankar R, Szentgyörgyi E, Laffitte A, Mauro MS, Audhya A, Müller-Reichert T, Bahmanyar S. Regulated lipid synthesis and LEM2/CHMP7 jointly control nuclear envelope closure. Journal Of Cell Biology 2020, 219: e201908179. PMID: 32271860, PMCID: PMC7199858, DOI: 10.1083/jcb.201908179.Peer-Reviewed Original ResearchConceptsER membraneNuclear permeability barrierESCRT-III componentsC. elegans oocytesMeiotic spindle microtubulesDe novo glycerolipid synthesisPermeability barrierGlycerolipid synthesisESCRT componentsESCRT-IIIProtein phosphataseCytoplasmic membraneSpindle microtubulesNE permeabilityMeiotic spindleLipid synthesis