2024
Microbe Profile: Bacteriophage ϕ6: a model for segmented RNA viruses and the evolutionary consequences of viral ‘sex’
Turner P, Chao L. Microbe Profile: Bacteriophage ϕ6: a model for segmented RNA viruses and the evolutionary consequences of viral ‘sex’. Microbiology 2024, 170: 001467. PMID: 39046321, PMCID: PMC11316545, DOI: 10.1099/mic.0.001467.Peer-Reviewed Original ResearchConceptsEukaryotic virusesHost-pathogen interactionsEvolution of sexDsRNA virusesEvolutionary consequencesSegment reassortmentBacterial virusesRNA virusesRate of adaptationMutation ratePhenotypic complexityMutation loadLipid envelopeBacteriophageReassortmentRNAMutationsVirusMolecular modelingDsRNAExperimental systemGeneticsTraitsHostReplicationExperimental Evolution Studies in Φ6 Cystovirus
Singhal S, Balitactac A, Nayagam A, Bahrami P, Nayeem S, Turner P. Experimental Evolution Studies in Φ6 Cystovirus. Viruses 2024, 16: 977. PMID: 38932268, PMCID: PMC11209170, DOI: 10.3390/v16060977.Peer-Reviewed Original ResearchConceptsExperimental evolution studiesFitness effects of spontaneous mutationsSpontaneous mutationsEffects of spontaneous mutationsEvolution of RNA virusesEvolution studiesEvolution of host rangeFast generation timesResponse to selectionMultiple phagesNon-pathogenic modelRNA genomeConsequence of evolutionOuter membraneHost rangeRNA virusesNovel traitsMutation rateHost cellsFitness effectsPopulation sizeMutationsCystovirusesBacteriophageGeneration time
2020
Experimental Evolution of Human Rhinovirus Strains Adapting to Mouse Cells
Wasik B, Wasik B, Foxman E, Iwasaki A, Turner P. Experimental Evolution of Human Rhinovirus Strains Adapting to Mouse Cells. Genetic And Evolutionary Computation 2020, 145-157. DOI: 10.1007/978-3-030-39831-6_12.Peer-Reviewed Original ResearchMouse cellsIdentical selection pressuresExperimental evolution studiesLaboratory tissue cultureCommon cold illnessesViral capsid geneMolecular divergenceExperimental evolutionReplication genesSelection pressureRelated populationsGenetic changesRNA virusesHuman rhinovirus strainsCapsid geneEvolution studiesRV-1BInnate immunityGenesTissue cultureDifferent strainsCellsLA-4 cellsHostMouse host
2016
Reassortment in segmented RNA viruses: mechanisms and outcomes
McDonald SM, Nelson MI, Turner PE, Patton JT. Reassortment in segmented RNA viruses: mechanisms and outcomes. Nature Reviews Microbiology 2016, 14: 448-460. PMID: 27211789, PMCID: PMC5119462, DOI: 10.1038/nrmicro.2016.46.Peer-Reviewed Original ResearchConceptsRNA virusesGenome segmentsProtein-RNA interactionsMultiple selection pressuresRNA-RNA interactionsSingle host cellSegmented RNA virusViral fitnessDifferent gene segmentsProtein setsSelection pressureHost cellsGene segmentsGeneration of reassortantsImportant pathogenReassortmentDifferent viral strainsReassortant virusesFitnessImmune recognitionHybrid virionsReassortantsVirusMore virusesPlantsGeneralized selection to overcome innate immunity selects for host breadth in an RNA virus
Wasik BR, Muñoz‐Rojas A, Okamoto KW, Miller‐Jensen K, Turner PE. Generalized selection to overcome innate immunity selects for host breadth in an RNA virus. Evolution 2016, 70: 270-281. PMID: 26882316, DOI: 10.1111/evo.12845.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virus populationsInnate immunityRNA virusesHost cell levelVirus-host coevolutionDifferent species originViral fitnessCompetent cellsVSV populationsHost breadthHuman cancer cellsEvolutionary historyFitness differencesHost speciesDisparate hostsSpecies originInfection successInnate immune capacityPrimate cellsInnate immune functionVirus populationsImmune deficient cellsEmergence potentialImmune competent cellsNonhuman primate cells
2015
Rate of novel host invasion affects adaptability of evolving RNA virus lineages
Morley VJ, Mendiola SY, Turner PE. Rate of novel host invasion affects adaptability of evolving RNA virus lineages. Proceedings Of The Royal Society B 2015, 282: 20150801. PMID: 26246544, PMCID: PMC4632612, DOI: 10.1098/rspb.2015.0801.Peer-Reviewed Original ResearchConceptsNovel hostHost cell typesHost invasionCell typesVirus lineagesVirus populationsGenomic outcomesEnvironmental turnoverTissue culture environmentAbility of virusesHost typeRNA virusesConsensus sequencingInfluences adaptationLineagesGradual invasionHostHistorical contingencyMolecular substitutionDifferent genotypesAdaptive changesInvasionRapid shiftVirusSequencing
2013
STOCHASTIC TEMPERATURES IMPEDE RNA VIRUS ADAPTATION
Alto BW, Wasik BR, Morales NM, Turner PE. STOCHASTIC TEMPERATURES IMPEDE RNA VIRUS ADAPTATION. Evolution 2013, 67: 969-979. PMID: 23550749, DOI: 10.1111/evo.12034.Peer-Reviewed Original ResearchConceptsFitness gainsVesicular stomatitis virusEvolution of generalistsConvergent molecular evolutionEvolution of specializationCell-binding proteinsMolecular evolutionGenome substitutionsConstant environmentVirus adaptationSequence analysisRNA virusesG geneVirus populationsStomatitis virusMolecular changesLineagesGeneralistsTemperature treatmentKey roleEvolutionGenesLociVirusProtein
2012
Selective Pressure Causes an RNA Virus to Trade Reproductive Fitness for Increased Structural and Thermal Stability of a Viral Enzyme
Dessau M, Goldhill D, McBride R, Turner PE, Modis Y. Selective Pressure Causes an RNA Virus to Trade Reproductive Fitness for Increased Structural and Thermal Stability of a Viral Enzyme. PLOS Genetics 2012, 8: e1003102. PMID: 23209446, PMCID: PMC3510033, DOI: 10.1371/journal.pgen.1003102.Peer-Reviewed Original ResearchConceptsFitness tradeoffsPleiotropic mutationMolecular basisRNA virusesSingle amino acid substitutionAmino acid substitutionsIndividual point mutationsEvolutionary tugPleiotropic phenotypesExperimental evolutionAntagonistic pleiotropyEvolutionary biologyProtein functionLytic transglycosylaseNatural selectionProtein P5Reproductive fitnessStructural biologySelective pressureMutational substitutionsHeat shockProtein structureCapsid assemblyAcid substitutionsBacteriophage Φ6
2010
ROLE OF EVOLVED HOST BREADTH IN THE INITIAL EMERGENCE OF AN RNA VIRUS
Turner PE, Morales NM, Alto BW, Remold SK. ROLE OF EVOLVED HOST BREADTH IN THE INITIAL EMERGENCE OF AN RNA VIRUS. Evolution 2010, 64: 3273-3286. PMID: 20633045, DOI: 10.1111/j.1558-5646.2010.01051.x.Peer-Reviewed Original ResearchConceptsHost breadthNew hostHost growthPathogen emergenceDirect selectionVesicular stomatitis virus populationsType of selectionHost shiftsPhenotypic plasticityNiche breadthSpecialist virusesHost colonizationIndirect selectionDifferent hostsPopulation varianceRNA virusesPathogen infectiousnessGeneralistsVirus populationsHostEnvironmental robustnessFortuitous changesPopulation growthGrowthVirus
2009
Consequences of host adaptation for performance of vesicular stomatitis virus in novel thermal environments
Alto B, Turner P. Consequences of host adaptation for performance of vesicular stomatitis virus in novel thermal environments. Evolutionary Ecology 2009, 24: 299-315. DOI: 10.1007/s10682-009-9307-3.Peer-Reviewed Original ResearchHost adaptationVesicular stomatitis virusRNA virusesNovel thermal environmentThermal reaction normsStomatitis virusGeneralist traitsHost generalistsFitness traitsNiche specializationGeneralist organismsSpecies biodiversityReaction normsSelective advantageViral genesGeneralistsMultiple hostsViral proteinsSingle hostTraitsExtreme temperaturesWarm environmentNew environmentHostAdaptation
2008
Robustness promotes evolvability of thermotolerance in an RNA virus
McBride RC, Ogbunugafor CB, Turner PE. Robustness promotes evolvability of thermotolerance in an RNA virus. BMC Ecology And Evolution 2008, 8: 231. PMID: 18694497, PMCID: PMC2518931, DOI: 10.1186/1471-2148-8-231.Peer-Reviewed Original ResearchConceptsGenetic robustnessEfficacy of selectionNew habitatsEvolutionary biologyVirus adaptabilityLaboratory populationsMutational changesNew nichesEvolvabilityRNA virusesFace of perturbationsGreater toleranceThermotoleranceRobust clonesNovel environmentClonesBiological systemsBalance of robustnessFuture adaptationUnderlying mechanismHabitatsNicheBiologyGreater resistancePopulationEvolutionary Genomics of Host Adaptation in Vesicular Stomatitis Virus
Remold SK, Rambaut A, Turner PE. Evolutionary Genomics of Host Adaptation in Vesicular Stomatitis Virus. Molecular Biology And Evolution 2008, 25: 1138-1147. PMID: 18353798, DOI: 10.1093/molbev/msn059.Peer-Reviewed Original ResearchConceptsGenetic architectureComplex traitsHigher fitnessVirus populationsVesicular stomatitis virus populationsParallel phenotypic changesSimilar selection pressuresRNA virus populationsHigh mutation rateEvolutionary genomicsGenome evolutionHost useComplete consensus sequenceTrue pleiotropyHost adaptationAllele substitutionGenomic changesSelection pressureVesicular stomatitis virusConsensus sequenceMutation rateEcological historyPhenotypic changesRNA virusesHeLa cells
2007
EVOLUTION OF HOST SPECIFICITY DRIVES REPRODUCTIVE ISOLATION AMONG RNA VIRUSES
Duffy S, Burch CL, Turner PE. EVOLUTION OF HOST SPECIFICITY DRIVES REPRODUCTIVE ISOLATION AMONG RNA VIRUSES. Evolution 2007, 61: 2614-2622. PMID: 17908251, PMCID: PMC7202233, DOI: 10.1111/j.1558-5646.2007.00226.x.Peer-Reviewed Original ResearchConceptsReproductive isolationEcological speciation hypothesisDivergent natural selectionRNA virusesAssortative mating evolvesNatural selectionHost rangeRNA bacteriophage phi6Speciation hypothesisSingle nucleotide changePhytophagous insectsEcological adaptationNovel hostEvolutionary fateWhole-genome sequencingGenetic exchangeImportant traitsBiological credibilityAlternative hostsExperimental populationsLaboratory populationsAssortative matingDirect selectionGenome sequencingNucleotide changes
2006
Pleiotropic Costs of Niche Expansion in the RNA Bacteriophage Φ6
Duffy S, Turner PE, Burch CL. Pleiotropic Costs of Niche Expansion in the RNA Bacteriophage Φ6. Genetics 2006, 172: 751-757. PMID: 16299384, PMCID: PMC1456241, DOI: 10.1534/genetics.105.051136.Peer-Reviewed Original ResearchConceptsNiche expansionAntagonistic pleiotropyCosts of generalismVaried ecological conditionsRNA virus phi6Standard laboratory hostRNA bacteriophage φ6Host range mutantsPleiotropic costsHost shiftsPseudomonas hostsFitness costsGenetic basisGenetic dataHost attachmentHost rangeExperimental model systemAdaptive mutationsHost typeNonsynonymous mutationsEcological conditionsOriginal hostRNA virusesBacteriophage Φ6Generalism
2005
Evolution of Mutational Robustness in an RNA Virus
Montville R, Froissart R, Remold SK, Tenaillon O, Turner PE. Evolution of Mutational Robustness in an RNA Virus. PLOS Biology 2005, 3: e381. PMID: 16248678, PMCID: PMC1275523, DOI: 10.1371/journal.pbio.0030381.Peer-Reviewed Original ResearchConceptsMutational robustnessRNA virusesAccurate genome replicationEvolution of robustnessUnderstanding of evolutionAdaptive evolutionGenetic robustnessPopulation bottleneckingPhenotypic constancyMutation accumulationNatural selectionGenetic variationMutational effectsGenome replicationHigh mutation frequencyMutational changesRandom mutationsMutation rateHost cellsBiological populationsFitness changesMutation frequencyGenomeComplementationVirus
2001
Transmission bottlenecks and the evolution of fitness in rapidly evolving RNA viruses
Elena S, Sanjuán R, Borderı́a A, Turner P. Transmission bottlenecks and the evolution of fitness in rapidly evolving RNA viruses. Infection Genetics And Evolution 2001, 1: 41-48. PMID: 12798049, DOI: 10.1016/s1567-1348(01)00006-5.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virusRNA virusesEvolution of fitnessBasic genetic principlesNatural populationsGenetic driftEvolutionary importanceFitness lossCellular hostsTransmission bottleneckDeleterious mutationsHost individualsGenetic principlesBottleneck sizeViral adaptationViral fitnessStomatitis virusRandom accumulationSimilar bottlenecksTissue cultureMutationsFitnessProfound effectVirusMode of transmission
2000
Cost of Host Radiation in an RNA Virus
Turner P, Elena S. Cost of Host Radiation in an RNA Virus. Genetics 2000, 156: 1465-1470. PMID: 11102349, PMCID: PMC1461356, DOI: 10.1093/genetics/156.4.1465.Peer-Reviewed Original ResearchConceptsHost radiationNovel hostVesicular stomatitis virusOriginal hostHost environmentSubstantial fitness gainsMammalian host cellsNovel habitatsMultiple habitatsEcological nichesExperimental populationsFitness gainsWeak selectionHost typeNew hostGenetic materialHost cellsRNA virusesMultiple host typesReduced competitivenessStomatitis virusHabitatsSlow replicationHostFitnessThe Two Faces of Mutation: Extinction and Adaptation in RNA Viruses
Elena S, Miralles R, Cuevas J, Turner P, Moya A. The Two Faces of Mutation: Extinction and Adaptation in RNA Viruses. IUBMB Life 2000, 49: 5-9. PMID: 10772334, DOI: 10.1080/713803585.Peer-Reviewed Original ResearchConceptsRNA virusesPopulation sizeGenetic variabilityDrastic fitness lossFace of mutationCellular DNA replicationHigh genetic variabilityEffects of mutationsLarge population sizesViral population sizeBottleneck eventsGenetic driftFitness lossDNA replicationFitness gainsSegment exchangeDifferent tissuesViral extinctionReplicative intermediatesInfected hostExtinctionMutationsPopulation standpointHostReplication
1998
Sex and the Evolution of Intrahost Competition in RNA Virus φ6
Turner P, Chao L. Sex and the Evolution of Intrahost Competition in RNA Virus φ6. Genetics 1998, 150: 523-532. PMID: 9755186, PMCID: PMC1460345, DOI: 10.1093/genetics/150.2.523.Peer-Reviewed Original ResearchConceptsIntrahost competitionLarge sexual populationsLarge asexual populationsRNA virus phi6Absence of sexSame host cellRate of adaptationSexual populationsGenetic conflictSexual reproductionAsexual populationsMore rapid adaptationBeneficial mutationsSame genomeSeparate lineagesExperimental populationsBacterial hostsMultiple bacteriophagesAbility of virusesHost cellsRNA virusesRapid adaptationPseudomonas phaseolicolaPhi6Host