2012
Surface modified poly(β amino ester)-containing nanoparticles for plasmid DNA delivery
Fields RJ, Cheng CJ, Quijano E, Weller C, Kristofik N, Duong N, Hoimes C, Egan ME, Saltzman WM. Surface modified poly(β amino ester)-containing nanoparticles for plasmid DNA delivery. Journal Of Controlled Release 2012, 164: 41-48. PMID: 23041278, PMCID: PMC3625648, DOI: 10.1016/j.jconrel.2012.09.020.Peer-Reviewed Original ResearchConceptsTransfection efficiencyPDNA loadingNovel nanoparticle systemPlasmid DNA loadingGene delivery vehiclesGene therapy applicationsPlasmid DNA deliverySurface coatingSurface chargePBAE polymerCoated nanoparticlesNanoparticle systemsLiposomal vectorsUnmodified particlesDNA deliveryDNA loadingPDNA deliveryTherapy applicationsCellular internalizationDelivery vehiclesUnmodified NPsPBAEIntracellular uptakeBlended particlesNanoparticles
1996
Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line.
Kearns W, Afione S, Fulmer S, Pang M, Erikson D, Egan M, Landrum M, Flotte T, Cutting G. Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line. Gene Therapy 1996, 3: 748-55. PMID: 8875221.Peer-Reviewed Original ResearchConceptsIB3-1 cellsWild-type AAVEpithelial cell lineMetaphase spreadsSite-specific fashionCFTR cDNACell linesLow molecular weight DNA fractionCF bronchial epithelial cell lineHuman chromosome 19Immortalized epithelial cell lineWeight DNA fractionMetaphase chromosome preparationsMetaphase chromosome spreadsBronchial epithelial cell lineDifferent chromosomesAAV-CFTR vectorCDNA integrationChromosome spreadsChromosome 19Genomic DNASite-specific mannerDNA fractionChromosome preparationsCFTR expression