2002
Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits
Chatterton JE, Awobuluyi M, Premkumar LS, Takahashi H, Talantova M, Shin Y, Cui J, Tu S, Sevarino KA, Nakanishi N, Tong G, Lipton SA, Zhang D. Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature 2002, 415: 793-798. PMID: 11823786, DOI: 10.1038/nature715.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCations, DivalentCentral Nervous SystemCloning, MolecularElectrophysiologyGlutamic AcidGlycineImmunohistochemistryIn Situ HybridizationIon TransportMagnesiumMolecular Sequence DataNeuronsN-MethylaspartateOocytesProtein SubunitsRatsReceptors, GlycineReceptors, N-Methyl-D-AspartateRNA, MessengerSequence HomologySerineSubstrate SpecificityXenopus laevisConceptsExcitatory glycine receptorsGlycine receptorsN-methyl-D-aspartate (NMDA) subtypeD-serineCentral nervous systemNMDA receptor subunitsImpermeable cation channelsVoltage-dependent inhibitionBursts of firingMK-801Inhibitory neurotransmitterMotor neuronsGlutamate receptorsCerebrocortical neuronsCompetitive antagonistNervous systemDual agonistsNMDARNMDAR familyReceptor subunitsNeuronal developmentPathological processesCation channelsReceptorsNR3A
1997
An anti-χ-1 antibody recognizes a heavily glycosylated protein in rat brain
Ciabarra A, Sevarino K. An anti-χ-1 antibody recognizes a heavily glycosylated protein in rat brain. Brain Research 1997, 46: 85-90. PMID: 9191081, DOI: 10.1016/s0169-328x(96)00275-6.Peer-Reviewed Original Research
1996
Preprothyrotropin-releasing hormone mRNA in the rat central gray is strongly and persistently induced during morphine withdrawal
Gahn L, Sevarino K. Preprothyrotropin-releasing hormone mRNA in the rat central gray is strongly and persistently induced during morphine withdrawal. Neuropeptides 1996, 30: 207-212. PMID: 8819143, DOI: 10.1016/s0143-4179(96)90065-7.Peer-Reviewed Original ResearchConceptsThyrotropin-releasing hormoneVentrolateral central grayCentral grayOpiate withdrawalMorphine withdrawalAcute naltrexone treatmentPrecipitation of withdrawalHormone mRNAChronic morphineNaltrexone treatmentSystemic administrationOpiate dependencePhysical symptomsVivo evidenceWithdrawalMorphineSignificant increaseRatsSitu hybridizationMRNA
1995
Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family
Ciabarra A, Sullivan J, Gahn L, Pecht G, Heinemann S, Sevarino K. Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family. Journal Of Neuroscience 1995, 15: 6498-6508. PMID: 7472412, PMCID: PMC6577996, DOI: 10.1523/jneurosci.15-10-06498.1995.Peer-Reviewed Original ResearchConceptsAgonist-activated currentsGlutamate receptor subunitsIonotropic glutamate receptor familyGlutamate receptor familyReceptor subunitsReceptor familyPostnatal day 1First postnatal weekNMDA receptor subunitsFunctional NMDA receptorsIonotropic glutamate receptorsSpinal cordCA1 fieldHeteromeric configurationsNMDA receptorsGlutamate receptorsPostnatal weekNMDA subunitsDay 1Functional expression studiesNeuronal signalingPrecise roleReceptorsTranscript levelsXenopus oocytesRegulation of Cytochrome c Oxidase Subunit mRNA and Enzyme Activity in Rat Brain Reward Regions During Withdrawal from Chronic Cocaine
Walker J, Sevarino K. Regulation of Cytochrome c Oxidase Subunit mRNA and Enzyme Activity in Rat Brain Reward Regions During Withdrawal from Chronic Cocaine. Journal Of Neurochemistry 1995, 64: 497-502. PMID: 7830041, DOI: 10.1046/j.1471-4159.1995.64020497.x.Peer-Reviewed Original ResearchConceptsMedial prefrontal cortexNucleus accumbensBrain regionsChronic cocaine treatmentCO activityChronic treatmentCocaine treatmentBrain reward regionsChronic cocaineDrug rewardPrefrontal cortexSubunit mRNAsIndividual ratsReward regionsCocaineNorthern blot analysisBlot analysisTime-dependent patternUnique time-dependent patternEnzyme activityRatsScreening procedureTreatmentMRNAMetabolic activity
1993
Cocaine Regulation of Brain Preprothyrotropin‐Releasing Hormone mRNA
Sevarino K, Primus R. Cocaine Regulation of Brain Preprothyrotropin‐Releasing Hormone mRNA. Journal Of Neurochemistry 1993, 60: 1151-1154. PMID: 8436966, DOI: 10.1111/j.1471-4159.1993.tb03267.x.Peer-Reviewed Original ResearchConceptsThyrotropin-releasing hormoneCocaine treatmentNucleus accumbensCocaine actionSolution hybridization RNase protectionMRNA levelsRat brain regionsChronic cocaine treatmentAcute cocaine treatmentPreclinical evidenceExtrahypothalamic regionsCocaine withdrawalMin postinjectionH postinjectionPrepro-TRHBrain regionsCocaine regulationCocaine reinforcementAmygdalaThalamusTRH precursorHippocampusHormone mRNASignificant decreaseRNase protection
1991
Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways.
Sevarino K, Stork P. Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways. Journal Of Biological Chemistry 1991, 266: 18507-18513. PMID: 1680862, DOI: 10.1016/s0021-9258(18)55090-0.Peer-Reviewed Original ResearchConceptsRegulated secretory pathwaySecretory pathwayMutant precursorRat preprosomatostatinVesicular stomatitis virus G proteinMature peptide regionVirus G proteinSorting signalsRegulated secretionAmino terminusProteolytic maturationExpression vectorNeuropeptide precursorsSite recognitionG proteinsRIN-5F cellsPeptide regionPathwaySecreted productsMutationsIntracellular productsCellsEndocrine cellsResiduesA general role for adaptations in G-proteins and the cyclic AMP system in mediating the chronic actions of morphine and cocaine on neuronal function
Terwilliger R, Beitner-Johnson D, Sevarino K, Crain S, Nestler E. A general role for adaptations in G-proteins and the cyclic AMP system in mediating the chronic actions of morphine and cocaine on neuronal function. Brain Research 1991, 548: 100-110. PMID: 1651140, DOI: 10.1016/0006-8993(91)91111-d.Peer-Reviewed Original ResearchConceptsCyclic AMP systemChronic morphineNucleus accumbensBrain regionsAdenylate cyclaseAMP systemDorsal root ganglion/spinal cordG proteinsRat locus coeruleusMorphine regulationChronic treatmentLocus coeruleusSpinal cordChronic actionsOpiate toleranceAbused substancesMorphineAMP-dependent protein kinase activityCyclic AMP-dependent protein kinase activityNeuronal functionKinase activityDecreased levelsDrug addictionCocaineThalamus
1990
Processing and intracellular sorting of anglerfish and rat preprosomatostatins in mammalian endocrine cells
Sevarino K, Ventimiglia R, Stork P. Processing and intracellular sorting of anglerfish and rat preprosomatostatins in mammalian endocrine cells. Metabolism 1990, 39: 26-29. PMID: 1976215, DOI: 10.1016/0026-0495(90)90203-o.Peer-Reviewed Original ResearchChronic Cocaine Treatment Decreases Levels of the G Protein Subunits Giα and Goα in Discrete Regions of Rat Brain
Nestler E, Terwilliger R, Walker J, Sevarino K, Duman R. Chronic Cocaine Treatment Decreases Levels of the G Protein Subunits Giα and Goα in Discrete Regions of Rat Brain. Journal Of Neurochemistry 1990, 55: 1079-1082. PMID: 2117048, DOI: 10.1111/j.1471-4159.1990.tb04602.x.Peer-Reviewed Original ResearchConceptsVentral tegmental areaChronic cocaineTegmental areaNucleus accumbensBrain regionsLocus coeruleusGi alphaPertussis toxin-mediated ADP-ribosylationRat brain regionsTreatment of ratsG proteinsG protein ADP-ribosylationADP-ribosylation levelsElectrophysiological actionsBeta immunoreactivityADP-ribosylationChronic actionsRat brainCocaine actionGs alphaDecrease levelsAlpha levelsAccumbensImmunoreactivityCocaine
1989
Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity
Sevarino K, Stork P, Ventimiglia R, Mandel G, Goodman R. Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity. Cell 1989, 57: 11-19. PMID: 2564811, DOI: 10.1016/0092-8674(89)90167-0.Peer-Reviewed Original ResearchConceptsEndocrine cell linesRat preprosomatostatinCarboxy-terminal thirdDistinct cell typesAmino-terminal sequenceCell linesHybrid proteinLeader sequenceIntracellular targetingRegulated pathwayPreprosomatostatin 1Cellular factorsExpression vectorCell typesPattern of processingProcessing siteBioactive peptidesAnglerfish isletsSequenceDifferential processingPeptidesProteinResiduesPathwayHigh levelsCharacterization and Expression of the Gene‐Encoding Rat Thyrotropin‐Releasing Hormone (TRH)
LEE S, SEVARINO K, ROOS B, GOODMAN R. Characterization and Expression of the Gene‐Encoding Rat Thyrotropin‐Releasing Hormone (TRH). Annals Of The New York Academy Of Sciences 1989, 553: 14-28. PMID: 2497670, DOI: 10.1111/j.1749-6632.1989.tb54475.x.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsBase SequenceBlotting, NorthernBlotting, SouthernBrainCloning, MolecularDNAEnhancer Elements, GeneticGenesMolecular Sequence DataOrgan SpecificityPromoter Regions, GeneticProtein PrecursorsRatsRecombinant Fusion ProteinsRestriction MappingRNA, MessengerThyrotropin-Releasing HormoneTransfection
1988
Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line.
Sevarino K, Wu P, Jackson I, Roos B, Mandel G, Goodman R. Biosynthesis of thyrotropin-releasing hormone by a rat medullary thyroid carcinoma cell line. Journal Of Biological Chemistry 1988, 263: 620-623. PMID: 3121619, DOI: 10.1016/s0021-9258(19)35397-9.Peer-Reviewed Original ResearchConceptsHypothalamic tissueCA77 cellsThyrotropin-releasing hormoneThyroid carcinoma cell linesRat medullary thyroid carcinoma cell lineMedullary thyroid carcinoma cell lineHormone messenger RNAPost-translational maturationCarcinoma cell linesImmunoreactive TRHRat hypothalamusPromising model systemGene regulationTRH precursorFurther studiesCell linesMessenger RNACell processesModel systemRNATissueRelative levels
1987
Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells.
Sevarino K, Felix R, Banks C, Low M, Montminy M, Mandel G, Goodman R. Cell-specific processing of preprosomatostatin in cultured neuroendocrine cells. Journal Of Biological Chemistry 1987, 262: 4987-4993. PMID: 2881926, DOI: 10.1016/s0021-9258(18)61143-3.Peer-Reviewed Original Research
1986
Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
Montminy M, Sevarino K, Wagner J, Mandel G, Goodman R. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 6682-6686. PMID: 2875459, PMCID: PMC386573, DOI: 10.1073/pnas.83.18.6682.Peer-Reviewed Original ResearchConceptsRat somatostatin geneSomatostatin geneCAMP responsivenessReporter gene encoding chloramphenicolTranscriptional initiation siteProtein kinase 2Promoter deletion mutantsSimian virus 40 promoterSomatostatin gene promoterCAMP-responsive elementPC12 rat pheochromocytoma cellsKinase 2 activityGene encoding chloramphenicolTranscriptional regulationDeletion mutantsChimeric genePromoter elementsGene promoterGene expressionKinase 2Rat pheochromocytoma cellsInitiation siteResponsive elementGenesBase pairs