1991
Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways.
Sevarino K, Stork P. Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways. Journal Of Biological Chemistry 1991, 266: 18507-18513. PMID: 1680862, DOI: 10.1016/s0021-9258(18)55090-0.Peer-Reviewed Original ResearchConceptsRegulated secretory pathwaySecretory pathwayMutant precursorRat preprosomatostatinVesicular stomatitis virus G proteinMature peptide regionVirus G proteinSorting signalsRegulated secretionAmino terminusProteolytic maturationExpression vectorNeuropeptide precursorsSite recognitionG proteinsRIN-5F cellsPeptide regionPathwaySecreted productsMutationsIntracellular productsCellsEndocrine cellsResiduesA general role for adaptations in G-proteins and the cyclic AMP system in mediating the chronic actions of morphine and cocaine on neuronal function
Terwilliger R, Beitner-Johnson D, Sevarino K, Crain S, Nestler E. A general role for adaptations in G-proteins and the cyclic AMP system in mediating the chronic actions of morphine and cocaine on neuronal function. Brain Research 1991, 548: 100-110. PMID: 1651140, DOI: 10.1016/0006-8993(91)91111-d.Peer-Reviewed Original ResearchConceptsCyclic AMP systemChronic morphineNucleus accumbensBrain regionsAdenylate cyclaseAMP systemDorsal root ganglion/spinal cordG proteinsRat locus coeruleusMorphine regulationChronic treatmentLocus coeruleusSpinal cordChronic actionsOpiate toleranceAbused substancesMorphineAMP-dependent protein kinase activityCyclic AMP-dependent protein kinase activityNeuronal functionKinase activityDecreased levelsDrug addictionCocaineThalamus
1986
Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
Montminy M, Sevarino K, Wagner J, Mandel G, Goodman R. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 6682-6686. PMID: 2875459, PMCID: PMC386573, DOI: 10.1073/pnas.83.18.6682.Peer-Reviewed Original ResearchConceptsRat somatostatin geneSomatostatin geneCAMP responsivenessReporter gene encoding chloramphenicolTranscriptional initiation siteProtein kinase 2Promoter deletion mutantsSimian virus 40 promoterSomatostatin gene promoterCAMP-responsive elementPC12 rat pheochromocytoma cellsKinase 2 activityGene encoding chloramphenicolTranscriptional regulationDeletion mutantsChimeric genePromoter elementsGene promoterGene expressionKinase 2Rat pheochromocytoma cellsInitiation siteResponsive elementGenesBase pairs