1993
Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line.
Nillni E, Sevarino K, Jackson I. Identification of the thyrotropin-releasing hormone-prohormone and its posttranslational processing in a transfected AtT20 tumoral cell line. Endocrinology 1993, 132: 1260-70. PMID: 8440187, DOI: 10.1210/endo.132.3.8440187.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlotting, WesternElectrophoresis, Polyacrylamide GelKineticsMiceModels, GeneticMolecular Sequence DataMolecular WeightPeptide FragmentsPituitary NeoplasmsProtein PrecursorsProtein Processing, Post-TranslationalPyrrolidonecarboxylic AcidThyrotropin-Releasing HormoneTransfectionTrypsinTumor Cells, Cultured
1989
Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity
Sevarino K, Stork P, Ventimiglia R, Mandel G, Goodman R. Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity. Cell 1989, 57: 11-19. PMID: 2564811, DOI: 10.1016/0092-8674(89)90167-0.Peer-Reviewed Original ResearchConceptsEndocrine cell linesRat preprosomatostatinCarboxy-terminal thirdDistinct cell typesAmino-terminal sequenceCell linesHybrid proteinLeader sequenceIntracellular targetingRegulated pathwayPreprosomatostatin 1Cellular factorsExpression vectorCell typesPattern of processingProcessing siteBioactive peptidesAnglerfish isletsSequenceDifferential processingPeptidesProteinResiduesPathwayHigh levels