1991
Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways.
Sevarino K, Stork P. Multiple preprosomatostatin sorting signals mediate secretion via discrete cAMP- and tetradecanoylphorbolacetate-responsive pathways. Journal Of Biological Chemistry 1991, 266: 18507-18513. PMID: 1680862, DOI: 10.1016/s0021-9258(18)55090-0.Peer-Reviewed Original ResearchConceptsRegulated secretory pathwaySecretory pathwayMutant precursorRat preprosomatostatinVesicular stomatitis virus G proteinMature peptide regionVirus G proteinSorting signalsRegulated secretionAmino terminusProteolytic maturationExpression vectorNeuropeptide precursorsSite recognitionG proteinsRIN-5F cellsPeptide regionPathwaySecreted productsMutationsIntracellular productsCellsEndocrine cellsResidues[4] Assays for Biosynthesis of Thyrotropin-Releasing Hormone
Nillni E, Sevarino K, Wu P, Jackson I. [4] Assays for Biosynthesis of Thyrotropin-Releasing Hormone. International Review Of Neurobiology 1991, 6: 51-69. DOI: 10.1016/b978-0-12-185261-0.50009-5.Peer-Reviewed Original ResearchStandard molecular biological techniquesTranscriptional termination signalCloning siteMammalian cell linesMoloney mouse leukemia virusMolecular biological techniquesT-antigen geneRous sarcoma virusMammalian cellsTermination signalBamH1 fragmentPolyadenylation signalEcoR1 fragmentUnique cloning sitesPrecursor sequencesCytomegalovirus immediate early promoterImmediate early promoterMouse leukemia virusViral promotersExpression vectorSmall-T-antigen genePromoterSarcoma virusBiological techniquesAntigen gene
1989
Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity
Sevarino K, Stork P, Ventimiglia R, Mandel G, Goodman R. Amino-terminal sequences of prosomatostatin direct intracellular targeting but not processing specificity. Cell 1989, 57: 11-19. PMID: 2564811, DOI: 10.1016/0092-8674(89)90167-0.Peer-Reviewed Original ResearchConceptsEndocrine cell linesRat preprosomatostatinCarboxy-terminal thirdDistinct cell typesAmino-terminal sequenceCell linesHybrid proteinLeader sequenceIntracellular targetingRegulated pathwayPreprosomatostatin 1Cellular factorsExpression vectorCell typesPattern of processingProcessing siteBioactive peptidesAnglerfish isletsSequenceDifferential processingPeptidesProteinResiduesPathwayHigh levels