2005
Myocardin-related transcription factor B is required in cardiac neural crest for smooth muscle differentiation and cardiovascular development
Li J, Zhu X, Chen M, Cheng L, Zhou D, Lu MM, Du K, Epstein JA, Parmacek MS. Myocardin-related transcription factor B is required in cardiac neural crest for smooth muscle differentiation and cardiovascular development. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 8916-8921. PMID: 15951419, PMCID: PMC1157054, DOI: 10.1073/pnas.0503741102.Peer-Reviewed Original ResearchConceptsCardiac neural crest cellsNeural crest cellsNeural crestTranscription factorsMuscle differentiationSmooth muscle differentiationCrest cellsSmooth muscle cell differentiationTranscription factor BArch arteriesWnt-1 promoterCardiac outflow tract defectsMuscle cell differentiationMutant allele resultsNormal cardiovascular morphogenesisPremigratory neural crestCardiac neural crestGene trap micePattern of expressionCritical roleTranscriptional controlGene trapOutflow tract defectsAortic arch arteriesInsertional mutation
2004
Megakaryoblastic Leukemia Factor-1 Transduces Cytoskeletal Signals and Induces Smooth Muscle Cell Differentiation from Undifferentiated Embryonic Stem Cells*
Du KL, Chen M, Li J, Lepore JJ, Mericko P, Parmacek MS. Megakaryoblastic Leukemia Factor-1 Transduces Cytoskeletal Signals and Induces Smooth Muscle Cell Differentiation from Undifferentiated Embryonic Stem Cells*. Journal Of Biological Chemistry 2004, 279: 17578-17586. PMID: 14970199, DOI: 10.1074/jbc.m400961200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernCell DifferentiationCell NucleusCells, CulturedChromatinCOS CellsCytoplasmCytoskeletonDNA, ComplementaryDNA-Binding ProteinsEmbryo, MammalianGenes, DominantHumansImmunohistochemistryLuciferasesMiceMyocytes, Smooth MuscleNIH 3T3 CellsNuclear ProteinsOncogene Proteins, FusionPlasmidsPrecipitin TestsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProtein TransportReverse Transcriptase Polymerase Chain ReactionSignal TransductionStem CellsTissue DistributionTrans-ActivatorsTranscriptional ActivationTransfectionTwo-Hybrid System TechniquesConceptsSmooth muscle cell differentiationMuscle cell differentiationEmbryonic stem cellsPromoter/enhancerSmooth muscle cellsRhoA signalingSM22alpha promoterCell differentiationUndifferentiated embryonic stem cellsSerum response factorTranscription factor myocardinLeucine zipper domainTranscriptional regulatory elementsStem cellsMost human tissuesCytoskeletal signalsTranscriptional programsTransduce signalsMKL1 mutantSMC differentiationZipper domainRegulatory elementsActin polymerizationMKL1 geneSerum stimulation
2003
Myocardin Is a Critical Serum Response Factor Cofactor in the Transcriptional Program Regulating Smooth Muscle Cell Differentiation
Du KL, Ip HS, Li J, Chen M, Dandre F, Yu W, Lu MM, Owens GK, Parmacek MS. Myocardin Is a Critical Serum Response Factor Cofactor in the Transcriptional Program Regulating Smooth Muscle Cell Differentiation. Molecular And Cellular Biology 2003, 23: 2425-2437. PMID: 12640126, PMCID: PMC150745, DOI: 10.1128/mcb.23.7.2425-2437.2003.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell DifferentiationCells, CulturedCOS CellsDNA, ComplementaryGene Expression Regulation, DevelopmentalHumansMiceMicrofilament ProteinsMolecular Sequence DataMuscle ProteinsMuscle, SmoothMuscle, Smooth, VascularMyocardiumNuclear ProteinsOrgan SpecificityPromoter Regions, GeneticRatsRNA, Small InterferingSequence AlignmentSerum Response FactorStem CellsTrans-ActivatorsTranscriptional ActivationConceptsSerum response factorExpression of myocardinTranscriptional programsSerum Response Factor CofactorMyocardin geneSRF DNA-binding domainSmooth muscle cell lineageFunction of myocardinSmooth muscle cell differentiationDNA-binding domainMuscle cell lineageMuscle cell differentiationTranscription factor myocardinTranscriptional regulatory elementsUndifferentiated mouse ES cellsEmbryonic stem cellsMouse ES cellsAlpha promoter activitySmooth muscle alpha-actinSM22 alphaMuscle alpha-actinMutant proteinsVisceral SMCsEmbryonic developmentRegulatory elements
2001
Analysis of SM22α-Deficient Mice Reveals Unanticipated Insights into Smooth Muscle Cell Differentiation and Function
Zhang J, Kim S, Helmke B, Yu W, Du K, Lu M, Strobeck M, Yu Q, Parmacek M. Analysis of SM22α-Deficient Mice Reveals Unanticipated Insights into Smooth Muscle Cell Differentiation and Function. Molecular And Cellular Biology 2001, 21: 1336-1344. PMID: 11158319, PMCID: PMC99586, DOI: 10.1128/mcb.2001.21.4.1336-1344.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationEmbryonic and Fetal DevelopmentGene Expression Regulation, DevelopmentalGene TargetingLac OperonMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicMicrofilament ProteinsMuscle DevelopmentMuscle ProteinsMuscle, SmoothSignal TransductionTranscriptional ActivationConceptsVisceral SMCsBacterial lacZ reporter geneLocal mesenchymeWestern blot analysisSmooth muscle cell differentiationBeta-galactosidase activityBlood pressureControl littermatesHeart ratePostnatal developmentHomeostatic functionsHistological analysisMiceContractile SMCsBlot analysisDorsal aortaLittermatesAngiogenic programMuscle cell differentiationSM22alphaYolk sacCephalic mesenchymeCell differentiationLacZ reporter geneDetectable beta-galactosidase activity