2017
Mapping Protein–Protein Interactions Using Affinity Purification and Mass Spectrometry
Lee CM, Adamchek C, Feke A, Nusinow DA, Gendron JM. Mapping Protein–Protein Interactions Using Affinity Purification and Mass Spectrometry. Methods In Molecular Biology 2017, 1610: 231-249. PMID: 28439867, DOI: 10.1007/978-1-4939-7003-2_15.Peer-Reviewed Original ResearchConceptsAP-MSHigher-order protein complexesAffinity purificationMapping protein-protein interactionsProtein-protein interaction networkProtein-protein interactionsMeaningful complexesProtein interactomeProtein complexesProtein-specific antibodiesMass spectrometry technologyInteraction networksCellular pathwaysTransient interactionsMass spectrometryInteractomeNonspecific interactionsSpectrometry technologyUnbiased methodComplexesPurificationComprehensive knowledgeCrucial stepPlantsProtein
2016
Using the Ubiquitin-modified Proteome to Monitor Distinct and Spatially Restricted Protein Homeostasis Dysfunction*
Gendron JM, Webb K, Yang B, Rising L, Zuzow N, Bennett EJ. Using the Ubiquitin-modified Proteome to Monitor Distinct and Spatially Restricted Protein Homeostasis Dysfunction*. Molecular & Cellular Proteomics 2016, 15: 2576-2593. PMID: 27185884, PMCID: PMC4974337, DOI: 10.1074/mcp.m116.058420.Peer-Reviewed Original ResearchConceptsUbiquitylation eventsProtein homeostasisProtein populationHomeostasis dysfunctionGlobal protein homeostasisP97/VCPDistinct protein populationsQuantitative proteomics methodHomeostasis functionsUbiquitin-proteasome systemDistinct functional outcomesProteomic methodsProteasome systemSubcellular fractionationProteomeFluorescent reportersHuman pathologiesProteasome dysfunctionDistinct insultsUbReporterPotent inhibitorHomeostasisUnique alterationsKey role
2007
A Proteomics Study of Brassinosteroid Response in Arabidopsis *
Deng Z, Zhang X, Tang W, Oses-Prieto JA, Suzuki N, Gendron JM, Chen H, Guan S, Chalkley RJ, Peterman TK, Burlingame AL, Wang ZY. A Proteomics Study of Brassinosteroid Response in Arabidopsis *. Molecular & Cellular Proteomics 2007, 6: 2058-2071. PMID: 17848588, PMCID: PMC2966871, DOI: 10.1074/mcp.m700123-mcp200.Peer-Reviewed Original ResearchConceptsTwo-dimensional DIGEProteomic studiesBiosynthesis of hormonesSpecific cellular processesPrevious microarray studyBri1-116Bzr1-1DBrassinosteroid responsesBR regulationBR actionVesicle traffickingBR responsesCellular processesRT-PCR analysisPosttranslational modificationsBrassinosteroidsCytoskeleton rearrangementMolecular detailsProtein responseBR treatmentPlant steroidsBR mutantsMolecular networksDiverse processesPhysiological processes