2020
A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis
Feke AM, Hong J, Liu W, Gendron JM. A Decoy Library Uncovers U-Box E3 Ubiquitin Ligases That Regulate Flowering Time in Arabidopsis. Genetics 2020, 215: 699-712. PMID: 32434795, PMCID: PMC7337086, DOI: 10.1534/genetics.120.303199.Peer-Reviewed Original ResearchConceptsE3 ubiquitin ligasesUbiquitin ligasesU-box E3 ubiquitin ligasesReproductive developmentReverse genetic screenMOS4-associated complexDominant-negative strategyGenetic redundancyGenetic screenTransgenic populationsTargeted degradationLigasesUnidentified regulatorDevelopmental transitionsBiological processesGenetic characterizationU-boxBiochemical studiesWidespread importanceRegulatorArabidopsisOrganismsPlantsProteinComplexes
2019
Decoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function
Feke A, Liu W, Hong J, Li MW, Lee CM, Zhou EK, Gendron JM. Decoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function. ELife 2019, 8: e44558. PMID: 30950791, PMCID: PMC6483598, DOI: 10.7554/elife.44558.Peer-Reviewed Original ResearchConceptsE3 ubiquitin ligasesUbiquitin ligasesCircadian clockCircadian functionPlant E3 ubiquitin ligasesTransgenic Arabidopsis plantsNew potential regulatorsArabidopsis plantsRegulated degradationPlant developmentClock proteinsClock regulatorsFunctional redundancyE3 ubiquitinProtein degradationGenetic challengesLigasesPotential regulatorCircadian periodScreening platformUbiquitinRegulatorDecoysSplicingClock