The chitinase PfCHT1 from the human malaria parasite Plasmodium falciparum lacks proenzyme and chitin-binding domains and displays unique substrate preferences
Vinetz J, Dave S, Specht C, Brameld K, Xu B, Hayward R, Fidock D. The chitinase PfCHT1 from the human malaria parasite Plasmodium falciparum lacks proenzyme and chitin-binding domains and displays unique substrate preferences. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 14061-14066. PMID: 10570198, PMCID: PMC24190, DOI: 10.1073/pnas.96.24.14061.Peer-Reviewed Original ResearchMeSH KeywordsAcetylglucosamineAmino Acid SequenceAnimalsBase SequenceBinding SitesChitinChitinasesEnzyme ActivationEnzyme InhibitorsEnzyme PrecursorsGene ExpressionGenes, ProtozoanHumansHydrogen-Ion ConcentrationMalariaModels, MolecularMolecular Sequence DataPlasmodium falciparumProtein ConformationProtozoan ProteinsSequence Homology, Amino AcidSubstrate SpecificityTrisaccharidesConceptsP. gallinaceumHuman malaria transmissionMosquito midgut epitheliumChitinase geneHuman malaria parasite Plasmodium falciparumChitin-binding domainMalaria parasite Plasmodium falciparumPfCHT1PgCHT1Malaria transmissionParasite Plasmodium falciparumPeritrophic matrixSubstrate preferenceP. falciparum genome databasePlasmodium falciparumMosquito midgutOocyst developmentParasite invasionBlood mealActive recombinant enzymeP. falciparum genesUnique substrate preferenceDifferential sensitivityGenome databaseHexameric oligomersChitinases of human parasites and their implications as antiparasitic targets.
Shahabuddin M, Vinetz J. Chitinases of human parasites and their implications as antiparasitic targets. EXS 1999, 87: 223-34. PMID: 10906963, DOI: 10.1007/978-3-0348-8757-1_16.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiparasitic AgentsChitinChitinasesDrug DesignEnzyme InhibitorsHumansParasitesParasitic DiseasesVaccinesConceptsDevelopmental stage-specific mannerStage-specific mannerAmino acid sequenceChitin-containing structuresPathogenic parasitesAnimal diseasesAntiparasitic targetsChitinase geneInsect vectorsAcid sequenceSuccessful infectionChitinasesHuman parasitesParasitesNumber of humanGenesLife cycleChitinAnimal bodyPathogensRecent studiesHarsh conditionsVertebratesChitinaseOrganisms