2022
Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior
Alvarez M, Alonso D, Kadri S, Rufalco-Moutinho P, Bernardes I, de Mello A, Souto A, Carrasco-Escobar G, Moreno M, Gamboa D, Vinetz J, Conn J, Ribolla P. Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior. Parasites & Vectors 2022, 15: 106. PMID: 35346342, PMCID: PMC8961893, DOI: 10.1186/s13071-022-05219-5.Peer-Reviewed Original ResearchConceptsGenome-wide association studiesSingle nucleotide polymorphism (SNP) markersGenome-wide studiesLow-coverage whole-genome sequencingGenomic sequencing dataMajor malaria vectorCircadian rhythm genesFemale timingPopulation stratification analysisWhole-genome sequencingMicrogeographic scaleSNP markersPolymorphism markersPopulation structureSequencing dataAssociation studiesInsecticide resistanceDusk/dawnRhythm genesGenotype imputationGenesFemale mosquitoesMalaria vectorsBlood mealGenetic association
2018
Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon
Moreno M, Tong-Rios C, Orjuela-Sanchez P, Carrasco-Escobar G, Campo B, Gamboa D, Winzeler EA, Vinetz JM. Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon. ACS Infectious Diseases 2018, 4: 541-548. PMID: 29465219, PMCID: PMC5902790, DOI: 10.1021/acsinfecdis.7b00195.Peer-Reviewed Original ResearchConceptsPlasmodium vivax sporozoitesDonor seraPlasmodium vivax liver stagesStandard membrane feeding assaysMalaria-endemic settingsMembrane feeding assaysSporozoite productionInfectious blood mealInfected subjectsNaïve serumBlood-fed mosquitoesLiver stagesDonor bloodBlood samplesVaccine developmentMosquito midgutSerumBlood mealBloodMosquito survivalSporozoitesMosquitoesAnophelesAnticoagulantsInfection
2001
Disruption of Plasmodium falciparumChitinase Markedly Impairs Parasite Invasion of Mosquito Midgut
Tsai Y, Hayward R, Langer R, Fidock D, Vinetz J. Disruption of Plasmodium falciparumChitinase Markedly Impairs Parasite Invasion of Mosquito Midgut. Infection And Immunity 2001, 69: 4048-4054. PMID: 11349075, PMCID: PMC98468, DOI: 10.1128/iai.69.6.4048-4054.2001.Peer-Reviewed Original ResearchConceptsMosquito midgutChitinase geneAvian malaria parasite Plasmodium gallinaceumMalaria parasite Plasmodium gallinaceumMutant ookinetesGenome databasePeritrophic matrixGene productsIntracellular traffickingBp upstreamOokinete invasionStop codonParasite invasionChitinolytic activityPfCHT1MidgutOokinetesOokinete formationGenesConfocal microscopyBlood mealApical endPlasmodium gallinaceumP. falciparumInvasion
2000
Chitinases of the Avian Malaria Parasite Plasmodium gallinaceum, a Class of Enzymes Necessary for Parasite Invasion of the Mosquito Midgut*
Vinetz J, Valenzuela J, Specht C, Aravind L, Langer R, Ribeiro J, Kaslow D. Chitinases of the Avian Malaria Parasite Plasmodium gallinaceum, a Class of Enzymes Necessary for Parasite Invasion of the Mosquito Midgut*. Journal Of Biological Chemistry 2000, 275: 10331-10341. PMID: 10744721, DOI: 10.1074/jbc.275.14.10331.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsChickensChitinasesConsensus SequenceCulicidaeDigestive SystemEpithelial CellsGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHumansKineticsMalaria, AvianMolecular Sequence DataPlasmodium gallinaceumRecombinant ProteinsSequence AlignmentSequence Homology, Amino AcidConceptsAvian malaria parasite Plasmodium gallinaceumMalaria parasite Plasmodium gallinaceumChitin-containing peritrophic matrixMosquito midgutPlasmodium gallinaceumTransmission-blocking interventionsBlood mealPgCHT1Mosquito midgut invasionPlasmodium ookinetesMidgut invasionParasite invasionPotential targetPeritrophic matrixMidgut epitheliumInvasionParasitesGallinaceumOokinetesMealMicroMActivity profiles
1999
The chitinase PfCHT1 from the human malaria parasite Plasmodium falciparum lacks proenzyme and chitin-binding domains and displays unique substrate preferences
Vinetz J, Dave S, Specht C, Brameld K, Xu B, Hayward R, Fidock D. The chitinase PfCHT1 from the human malaria parasite Plasmodium falciparum lacks proenzyme and chitin-binding domains and displays unique substrate preferences. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 14061-14066. PMID: 10570198, PMCID: PMC24190, DOI: 10.1073/pnas.96.24.14061.Peer-Reviewed Original ResearchMeSH KeywordsAcetylglucosamineAmino Acid SequenceAnimalsBase SequenceBinding SitesChitinChitinasesEnzyme ActivationEnzyme InhibitorsEnzyme PrecursorsGene ExpressionGenes, ProtozoanHumansHydrogen-Ion ConcentrationMalariaModels, MolecularMolecular Sequence DataPlasmodium falciparumProtein ConformationProtozoan ProteinsSequence Homology, Amino AcidSubstrate SpecificityTrisaccharidesConceptsP. gallinaceumHuman malaria transmissionMosquito midgut epitheliumChitinase geneHuman malaria parasite Plasmodium falciparumChitin-binding domainMalaria parasite Plasmodium falciparumPfCHT1PgCHT1Malaria transmissionParasite Plasmodium falciparumPeritrophic matrixSubstrate preferenceP. falciparum genome databasePlasmodium falciparumMosquito midgutOocyst developmentParasite invasionBlood mealActive recombinant enzymeP. falciparum genesUnique substrate preferenceDifferential sensitivityGenome databaseHexameric oligomers