2023
HIF-1 regulates pathogenic cytotoxic T cells in lupus skin disease
Little A, Chen P, Vesely M, Khan R, Fiedler J, Garritano J, Islam F, McNiff J, Craft J. HIF-1 regulates pathogenic cytotoxic T cells in lupus skin disease. JCI Insight 2023, 8: e166076. PMID: 37526979, PMCID: PMC10543720, DOI: 10.1172/jci.insight.166076.Peer-Reviewed Original ResearchConceptsCutaneous lupus erythematosusLupus skin diseaseT cellsSkin diseasesCytotoxic signatureInflammatory infiltrateHIF-1Tissue damageKidney-infiltrating T cellsSkin-infiltrating T cellsAutoimmune skin diseaseHIF-1 inhibitionSkin tissue damageLupus erythematosusSystemic diseaseTissue inflammationGranzyme BMouse modelInflammatory gene programDiseaseProtein levelsInfiltratesSkin environmentGene programPresent study
2021
Type I Interferon–Activated STAT4 Regulation of Follicular Helper T Cell–Dependent Cytokine and Immunoglobulin Production in Lupus
Dong X, Antao OQ, Song W, Sanchez GM, Zembrzuski K, Koumpouras F, Lemenze A, Craft J, Weinstein JS. Type I Interferon–Activated STAT4 Regulation of Follicular Helper T Cell–Dependent Cytokine and Immunoglobulin Production in Lupus. Arthritis & Rheumatology 2021, 73: 478-489. PMID: 33512094, PMCID: PMC7914134, DOI: 10.1002/art.41532.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntibody FormationAutoantibodiesB-LymphocytesCase-Control StudiesCytokinesDisease Models, AnimalFemaleHumansImmunoglobulinsInterferon Type IInterferon-gammaInterleukinsLupus Erythematosus, SystemicMaleMice, Inbred MRL lprMiddle AgedRNA-SeqSTAT4 Transcription FactorT Follicular Helper CellsConceptsSystemic lupus erythematosusTfh-like cellsTfh cellsIL-21Human lupusDisease activityCytokine productionSTAT4 activationImmunoglobulin productionPathogenic B cell responsesCourse of lupusClinical disease activityT cell secretionLupus-prone miceHealthy control subjectsCourse of diseaseB cell responsesCytokine interleukin-21Potential therapeutic targetType I IFNB cell maturationSLE patientsPathogenic cytokinesLupus erythematosusInterleukin-21
2020
Kidney tissue hypoxia dictates T cell–mediated injury in murine lupus nephritis
Chen PM, Wilson PC, Shyer JA, Veselits M, Steach HR, Cui C, Moeckel G, Clark MR, Craft J. Kidney tissue hypoxia dictates T cell–mediated injury in murine lupus nephritis. Science Translational Medicine 2020, 12 PMID: 32269165, PMCID: PMC8055156, DOI: 10.1126/scitranslmed.aay1620.Peer-Reviewed Original ResearchConceptsHypoxia-inducible factor-1Lupus nephritisT cellsTissue hypoxiaT-cell-mediated injuryCell-mediated injuryHIF-1 blockadeKidney tissue hypoxiaSystemic lupus erythematosusHuman lupus nephritisMurine lupus nephritisRenal injuryAutoimmune injuryLupus erythematosusAutoimmune diseasesImmune cellsRenal tissueMurine modelTissue damageMore hypoxicNephritisInjuryLow oxygen tensionOxygen tensionFactor 1
2017
Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus.
Choi JY, Seth A, Kashgarian M, Terrillon S, Fung E, Huang L, Wang LC, Craft J. Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus. The Journal Of Immunology 2017, 198: 2578-2588. PMID: 28219887, PMCID: PMC5360548, DOI: 10.4049/jimmunol.1601687.Peer-Reviewed Original ResearchConceptsIL-21Systemic lupus erythematosusMurine lupusTfh cellsLupus erythematosusDisease ameliorationGerminal center B cell responsesTfh-B cell interactionsGC responseFollicular Th cellsIL-21 blockadeLupus-prone B6Progression of glomerulonephritisCell-derived cytokinesB cell responsesMemory B cell developmentPotential therapeutic strategyB cell proliferationB cell maturationGC B cellsOverall survivalOrgan injuryAutoantibody productionLatter cytokineTh cells
2016
AI-22 Single cell protein and transcriptional profiling of CD4+ follicular B helper T (TFH) and central memory (TCM) cells in SLE: physiological and pathological phenotypes
Kwak M, Choi J, Kim S, Chen Z, Lee G, Craft J, Fan R. AI-22 Single cell protein and transcriptional profiling of CD4+ follicular B helper T (TFH) and central memory (TCM) cells in SLE: physiological and pathological phenotypes. Lupus Science & Medicine 2016, 3: a11. DOI: 10.1136/lupus-2016-000179.22.Peer-Reviewed Original ResearchCentral memory cellsTCM cellsTfh cellsSLE patientsHealthy donorsT cellsFollicular B helper T cellsSystemic lupus erythematosusCirculation of patientsHelper T cellsSLE T cellsLupus kidneysHumoral autoimmunityTranscriptional profilingIL-21Lupus erythematosusCytokine producersHelper TMurine lupusHumoral immunityLymphoid organsEnd organsLupus researchPathological outcomesTherapeutic target
2015
Circulating Follicular Helper–Like T Cells in Systemic Lupus Erythematosus: Association With Disease Activity
Choi J, Ho J, Pasoto SG, Bunin V, Kim ST, Carrasco S, Borba EF, Gonçalves CR, Costa PR, Kallas EG, Bonfa E, Craft J. Circulating Follicular Helper–Like T Cells in Systemic Lupus Erythematosus: Association With Disease Activity. Arthritis & Rheumatology 2015, 67: 988-999. PMID: 25581113, PMCID: PMC4450082, DOI: 10.1002/art.39020.Peer-Reviewed Original ResearchConceptsTfh-like cellsSLE Disease Activity IndexSystemic lupus erythematosusInducible T-cell costimulatorPD-1 expressionDisease activityT cellsBehçet's diseaseSLE patientsLupus erythematosusBlood samplesT cell-B cell collaborationCentral memory T cellsExpression of CXCR5Disease activity indexGerminal center activityMemory T cellsDeath 1 proteinFollicular helper TT-cell costimulatorActive diseaseAntibody positivityDisease durationFollicular helperIL-21
2013
Nanogel-based delivery of mycophenolic acid ameliorates systemic lupus erythematosus in mice
Look M, Stern E, Wang QA, DiPlacido LD, Kashgarian M, Craft J, Fahmy TM. Nanogel-based delivery of mycophenolic acid ameliorates systemic lupus erythematosus in mice. Journal Of Clinical Investigation 2013, 123: 1741-1749. PMID: 23454752, PMCID: PMC3613921, DOI: 10.1172/jci65907.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusMedian survival timeMycophenolic acidLupus erythematosusImmune cellsLupus-prone NZB/W F1 miceNZB/W F1 miceSimilar therapeutic resultsW F1 miceSevere renal damageCD4 T cellsAntigen-presenting cellsLupus therapyRenal damageIL-12Immunosuppressant mycophenolic acidInflammatory cytokinesProphylactic useTherapeutic resultsF1 miceT cellsSurvival timeGreater dosesErythematosusImmunosuppression
2012
The pathogenesis of systemic lupus erythematosus—an update
Choi J, Kim ST, Craft J. The pathogenesis of systemic lupus erythematosus—an update. Current Opinion In Immunology 2012, 24: 651-657. PMID: 23131610, PMCID: PMC3508331, DOI: 10.1016/j.coi.2012.10.004.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusLupus erythematosusAberrant adaptive immune responseAdaptive immune responsesDisease-specific therapeutic targetsInnate immune mechanismsPathogenic autoantibodiesImmune mechanismsTissue injuryImmune responseTherapeutic targetB cellsDisease mechanismsErythematosusClinical biologyRecent studiesAutoantibodiesLupusSLEPathogenesisInjuryIL-7Rαlow memory CD8+ T cells are significantly elevated in patients with systemic lupus erythematosus
Kim JS, Cho BA, Sim JH, Shah K, Woo CM, Lee EB, Lee DS, Kang JS, Lee WJ, Park CG, Craft J, Kang I, Kim HR. IL-7Rαlow memory CD8+ T cells are significantly elevated in patients with systemic lupus erythematosus. Rheumatology 2012, 51: 1587-1594. PMID: 22661557, PMCID: PMC3418646, DOI: 10.1093/rheumatology/kes100.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntibodies, BlockingAntigens, CDCD8-Positive T-LymphocytesCoculture TechniquesCytotoxicity, ImmunologicFemaleFlow CytometryHumansImmunologic MemoryImmunophenotypingLupus Erythematosus, SystemicLymphocyte CountReceptors, ImmunologicReceptors, Interleukin-7Signaling Lymphocytic Activation Molecule FamilyConceptsEffector memoryFrequency of ILT cellsHealthy individualsHuman effector memoryTarget cellsSystemic lupus erythematosusPathogenesis of lupusSLE patientsMemory CD8Lupus erythematosusNK cellsCytotoxic functionPeripheral bloodDisease manifestationsPatientsCD48 antigenCytotoxic moleculesFlow cytometrySLECell populationsPotential roleLupusILCellular characteristicsFollicular helper T cells in immunity and systemic autoimmunity
Craft JE. Follicular helper T cells in immunity and systemic autoimmunity. Nature Reviews Rheumatology 2012, 8: 337-347. PMID: 22549246, PMCID: PMC3604997, DOI: 10.1038/nrrheum.2012.58.Peer-Reviewed Original ResearchConceptsFollicular helper T cellsHelper T cellsT cellsSelf-reactive B cell clonesT helper cell populationSystemic lupus erythematosusSystemic autoimmune diseaseNormal immune responseT cell helpB cell clonesB cell survivalLupus erythematosusAutoantibody productionAutoimmune diseasesSystemic autoimmunityImmune responseAutoimmunityKey regulatorErythematosusCellsDiseaseImmunityCaspase‐activated DNase is required for maintenance of tolerance to lupus nuclear autoantigens
Jog NR, Frisoni L, Shi Q, Monestier M, Hernandez S, Craft J, Prak ET, Caricchio R. Caspase‐activated DNase is required for maintenance of tolerance to lupus nuclear autoantigens. Arthritis & Rheumatism 2012, 64: 1247-1256. PMID: 22127758, PMCID: PMC3292632, DOI: 10.1002/art.33448.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusAutoimmune responseMouse modelApoptotic cellsNuclear autoantigensDNA antibody titersLupus-prone miceAbsence of CADMaintenance of toleranceAutoreactive B cellsAnti-DNA antibodiesApoptotic modificationsLupus developmentGeneration of autoantigensAntinuclear antibodiesLupus erythematosusAntibody titersAutoimmune diseasesActive caspase-3Apoptotic bodiesB cellsMiceAutoantigensCAD deficiencyCaspase-3
2011
Expansion of IL-7Ralow memory CD8 T cells in human lupus (101.19)
Lee N, Shah K, Shin M, Kim S, Craft J, Kang I. Expansion of IL-7Ralow memory CD8 T cells in human lupus (101.19). The Journal Of Immunology 2011, 186: 101.19-101.19. DOI: 10.4049/jimmunol.186.supp.101.19.Peer-Reviewed Original ResearchSystemic lupus erythematosusDevelopment of SLESLE Disease Activity IndexMemory CD8SLE patientsT cellsCell subsetsHealthy controlsImmunopathogenesis of SLEAbstract Systemic lupus erythematosusHomeostatic cytokine IL-15IL-7 receptor alphaMemory CD8 T cellsCytotoxic molecules perforinHomeostasis of CD8Disease activity indexRole of CD8Cytokine IL-15CD8 T cellsImmune complex depositionProduction of autoantibodiesInflammatory autoimmune disorderT cell receptorDisease activityLupus erythematosusDissecting the Immune Cell Mayhem That Drives Lupus Pathogenesis
Craft JE. Dissecting the Immune Cell Mayhem That Drives Lupus Pathogenesis. Science Translational Medicine 2011, 3: 73ps9. PMID: 21389262, PMCID: PMC3694130, DOI: 10.1126/scitranslmed.3002138.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusPathogenesis of SLEAutoimmune disease systemic lupus erythematosusDisease systemic lupus erythematosusSerious side effectsScience Translational MedicineLupus pathogenesisLupus erythematosusImmunosuppressive drugsCombination therapyCommon treatmentSide effectsImmune systemPathogenesisTherapyTranslational medicineErythematosusInflammationInterferonNeutrophils
2010
Epstein‐Barr virus promotes interferon‐α production by plasmacytoid dendritic cells
Quan TE, Roman RM, Rudenga BJ, Holers VM, Craft JE. Epstein‐Barr virus promotes interferon‐α production by plasmacytoid dendritic cells. Arthritis & Rheumatism 2010, 62: 1693-1701. PMID: 20178121, PMCID: PMC2885535, DOI: 10.1002/art.27408.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, NuclearDendritic CellsDose-Response Relationship, ImmunologicEnzyme-Linked Immunosorbent AssayFlow CytometryHerpesvirus 4, HumanHumansImmunity, HumoralInterferon-alphaLupus Erythematosus, SystemicMajor Histocompatibility ComplexStatistics, NonparametricToll-Like Receptor 7Toll-Like Receptor 9ConceptsPlasmacytoid dendritic cellsSystemic lupus erythematosusHuman plasmacytoid dendritic cellsIFNalpha productionDendritic cellsEpstein-Barr virus infectionToll-like receptor 9Interferon-alpha levelsPresence of EBVChronic viral infectionsElevated viral loadAbility of EBVInnate immune cellsEpstein-Barr virusMajor histocompatibility complex moleculesInnate immune responseEnzyme-linked immunosorbent assayHistocompatibility complex moleculesSLE patientsEBV DNATLR-7Lupus erythematosusViral loadReceptor 9Immune cellsDysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus
Shah K, Lee WW, Lee SH, Kim SH, Kang SW, Craft J, Kang I. Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus. Arthritis Research & Therapy 2010, 12: r53. PMID: 20334681, PMCID: PMC2888202, DOI: 10.1186/ar2964.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusSLE Disease Activity Index (SLEDAI) scorePeripheral blood mononuclear cellsTh17-polarizing cytokinesTh1 cell responsesDisease activityT cellsHealthy subjectsIL-17Th1 cellsCell responsesPhorbol myristate acetateTh17 cellsLupus erythematosusIL-6Plasma levelsDisease activity index scoreBalance of CD4Balance of Th17Expression of Th17Th17 cell responseActivity index scoreFrequency of CD4Chemokine receptor CCR4Blood mononuclear cells
2009
ICOS Controls Effector Function but Not Trafficking Receptor Expression of Kidney-Infiltrating Effector T Cells in Murine Lupus
Odegard JM, DiPlacido LD, Greenwald L, Kashgarian M, Kono DH, Dong C, Flavell RA, Craft J. ICOS Controls Effector Function but Not Trafficking Receptor Expression of Kidney-Infiltrating Effector T Cells in Murine Lupus. The Journal Of Immunology 2009, 182: 4076-4084. PMID: 19299705, PMCID: PMC2746004, DOI: 10.4049/jimmunol.0800758.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, Differentiation, T-LymphocyteAutoantibodiesAutoantigensB-LymphocytesCD4-Positive T-LymphocytesChemokine CXCL9Chemotaxis, LeukocyteCytokinesDisease Models, AnimalEnzyme-Linked Immunosorbent AssayFlow CytometryFluorescent Antibody TechniqueInducible T-Cell Co-Stimulator ProteinKidneyLupus Erythematosus, SystemicLymphocyte ActivationMiceMice, Inbred MRL lprMice, TransgenicP-SelectinReceptors, CCR5Receptors, CXCR3ConceptsCD4 T cellsT cellsPerivascular infiltratesP-selectin ligandsMurine lupusReceptor expressionEffector functionsAutoreactive CD4 T cellsKidney-infiltrating T cellsEffector CD4 T cellsChemokine protein levelsEffector cell numbersIgG autoantibody productionExpression of CXCR3Effector T cellsSystemic lupus erythematosusImmune complex glomerulonephritisCellular inflammatory responseAutoantibody depositionComplex glomerulonephritisLupus erythematosusAutoantibody productionInflammatory chemokinesInflammatory cytokinesLigands CXCL9Lupus immunotherapy using CD4 targeted nanoparticles (48.29)
Look M, Stern E, Wang Q, DiPlacido L, Craft J, Fahmy T. Lupus immunotherapy using CD4 targeted nanoparticles (48.29). The Journal Of Immunology 2009, 182: 48.29-48.29. DOI: 10.4049/jimmunol.182.supp.48.29.Peer-Reviewed Original ResearchNZB/W F1 miceW F1 miceCD4 T cellsImmunosuppressive drugsDisease progressionF1 miceT cellsImmunosuppressive drug dosageSystemic lupus erythematosusNanoparticle therapyConventional therapeutic regimensFrequency of dosageLupus erythematosusAutoantibody productionTherapeutic regimensWeekly treatmentTherapeutic featuresAnimal modelsB cellsDrug dosageTherapeutic efficacyLupusImmunotherapyTherapyMice
2008
Autoantibodies against cyclophilin in systemic lupus erythematosus and Lyme disease
KRATZ A, HARDING MW, CRAFT J, MACKWORTH-YOUNG C, HANDSCHUMACHER RE. Autoantibodies against cyclophilin in systemic lupus erythematosus and Lyme disease. Clinical & Experimental Immunology 2008, 90: 422-427. PMID: 1458678, PMCID: PMC1554566, DOI: 10.1111/j.1365-2249.1992.tb05862.x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAmino Acid IsomerasesAntibodies, Anti-IdiotypicAntibodies, MonoclonalAntibody SpecificityAutoantibodiesCarrier ProteinsChildEnzyme-Linked Immunosorbent AssayFamily HealthFemaleHumansImmunoglobulin GImmunoglobulin IdiotypesImmunoglobulin MLupus Erythematosus, SystemicLyme DiseaseMaleMiddle AgedPeptidylprolyl IsomeraseProtein DenaturationConceptsSystemic lupus erythematosusLupus erythematosusFirst-degree relativesLyme disease patientsMonoclonal IgM antibodyLupus patientsIgM antibodiesDisease patientsLepromatous leprosyImmune systemPatientsWestern blotLyme diseaseAntibodiesErythematosusAutoantibodiesHypergammaglobulinaemiaCyclophilinCyclosporinLeprosyDiseaseSerum
2006
Abrogation of skin disease in LUPUS‐prone MRL/FASlpr mice by means of a novel tylophorine analog
Choi J, Gao W, Odegard J, Shiah H, Kashgarian M, McNiff JM, Baker DC, Cheng Y, Craft J. Abrogation of skin disease in LUPUS‐prone MRL/FASlpr mice by means of a novel tylophorine analog. Arthritis & Rheumatism 2006, 54: 3277-3283. PMID: 17009262, DOI: 10.1002/art.22119.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusEnd-organ diseaseInflammatory skin diseaseMRL/Skin diseasesMRL/Faslpr miceFemale MRL/NF-kappaB inhibitorFlow cytometric analysisFaslpr miceAutoantibody titersIgG levelsLupus erythematosusLymph nodesRenal diseaseVehicle treatmentKidney diseaseHepatic toxicityTotal IgMTylophorine analogsAntichromatin autoantibodiesTherapeutic effectHistopathologic analysisMurine modelSignificant abrogationCHAPTER 28 Systemic Lupus Erythematosus: Immunologic Features
KANG I, CRAFT J. CHAPTER 28 Systemic Lupus Erythematosus: Immunologic Features. 2006, 357-367. DOI: 10.1016/b978-012595961-2/50031-7.Peer-Reviewed Original ResearchSystemic lupus erythematosusPlasmacytoid dendritic cellsT cellsAdaptive immunityImmune complexesAberrant innate immune responsesAdaptive immune cellsUpregulation of IFNSubsequent tissue injuryPeripheral blood cellsInnate immune responseActivation of complementRespective autoantigensDendritic cellsLupus erythematosusData support rolesImmune cellsSerologic hallmarkTissue injuryClinical diseaseImmune responseTarget organsFc receptorsB cellsImmune system