2023
Dynamic microtubules slow down during their shrinkage phase
Luchniak A, Kuo Y, McGuinness C, Sutradhar S, Orbach R, Mahamdeh M, Howard J. Dynamic microtubules slow down during their shrinkage phase. Biophysical Journal 2023, 122: 616-623. PMID: 36659852, PMCID: PMC9989939, DOI: 10.1016/j.bpj.2023.01.020.Peer-Reviewed Original Research
2022
The force required to remove tubulin from the microtubule lattice by pulling on its α-tubulin C-terminal tail
Kuo YW, Mahamdeh M, Tuna Y, Howard J. The force required to remove tubulin from the microtubule lattice by pulling on its α-tubulin C-terminal tail. Nature Communications 2022, 13: 3651. PMID: 35752623, PMCID: PMC9233703, DOI: 10.1038/s41467-022-31069-x.Peer-Reviewed Original ResearchCounting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations
Liao M, Kuo Y, Howard J. Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations. Molecular Biology Of The Cell 2022, 33: ar48. PMID: 35323029, PMCID: PMC9265152, DOI: 10.1091/mbc.e21-12-0618.Peer-Reviewed Original ResearchConceptsEnd-binding protein 1Spinning-disk confocal microscopyConfocal microscopySingle-molecule imagingComplex biological phenomenaFly larvaeLiving cellsCell surfaceMicrotubule endsBiological phenomenaProtein 1Brightness of fluorophoresCytoplasmic concentrationEpifluorescence microscopeBiological systemsSensory neuronsCellsSingle moleculesAbsolute numberMolecular numberTissueLarvaeMicrotubulesEGFPProtein
2020
Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes
Kuo YW, Howard J. Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes. Trends In Cell Biology 2020, 31: 50-61. PMID: 33183955, PMCID: PMC7749064, DOI: 10.1016/j.tcb.2020.10.004.Peer-Reviewed Original ResearchConceptsAAA ATPasesTissue morphogenesisCellular processesMicrotubule cytoskeletonCell divisionGrowth promotionBiophysical advancesSevering enzymesMicrotubule networkMolecular mechanismsStrong promoterMicrotubule growthNeuronal developmentShort filamentsMicrotubulesSpastinEnzymeSeveringFidgetinKataninCytoskeletonMorphogenesisPromoterProteinRecent work
2008
XMAP215 Is a Processive Microtubule Polymerase
Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J, Hyman AA. XMAP215 Is a Processive Microtubule Polymerase. Cell 2008, 132: 79-88. PMID: 18191222, PMCID: PMC2311386, DOI: 10.1016/j.cell.2007.11.043.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesBiological AssayCatalytic DomainCell DifferentiationCell EnlargementCell LineCytoskeletonDiffusionDimerizationFetal ProteinsForminsGreen Fluorescent ProteinsMicrofilament ProteinsMicroscopy, FluorescenceMicrotubule-Associated ProteinsMicrotubulesNuclear ProteinsPolymersProtein BindingProtein Structure, TertiaryProtein TransportSpodopteraSus scrofaTubulinXenopus laevisXenopus ProteinsConceptsFamily of proteinsSingle-molecule assaysActin polymerasesMicrotubule cytoskeletonProcessive polymeraseTubulin subunit additionDynamic microtubulesMicrotubule polymeraseMicrotubule growthCytoskeletal polymersXMAP215Plus endsMicrotubule latticePolymeraseSubunit additionCell proliferationFree tubulinTubulin dimersMicrotubulesCommon mechanismFast growthTip trackingGrowthCytoskeletonRapid assembly
2006
Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner
Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nature Cell Biology 2006, 8: 957-962. PMID: 16906145, DOI: 10.1038/ncb1462.Peer-Reviewed Original ResearchConceptsKinesin-8 proteinsLength-dependent depolymerizationYeast kinesin-8Length-dependent mannerMetazoan cellsMicrotubule cytoskeletonMitotic spindleDisassemble microtubulesSpindle lengthKinesin-8Kinesin-13Kip3pPlus endsMicrotubulesConstituent microtubulesLong microtubulesNew mechanismCytoskeletonFungiKinesinProteinSpindleCells