2001
Ex vivo evaluation of PBMNCs collected with a new cell separator
Snyder E, O'Donnell L, Dengler T, Pomper G, Velleca M, Dincecco D, Baril L, Min K, Gudino M, Bender J. Ex vivo evaluation of PBMNCs collected with a new cell separator. Transfusion 2001, 41: 940-949. PMID: 11452164, DOI: 10.1046/j.1537-2995.2001.41070940.x.Peer-Reviewed Original ResearchMeSH KeywordsBlood DonorsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell DivisionCell SeparationCytotoxicity Tests, ImmunologicEndothelium, VascularE-SelectinHematopoietic Stem Cell TransplantationHumansInfant, NewbornKiller Cells, NaturalLeukapheresisLeukocytes, MononuclearTime FactorsUmbilical VeinsConceptsDendritic cellsNK cellsCell separatorHealthy volunteer blood donorsMNC subsetsEndothelial cellsVolunteer blood donorsB cell subsetsCell surface markersAmicus SeparatorNK cytotoxicityBlood donorsSelectin expressionAppropriate cell surface markersCD8CD4Ex vivoSurface markersCD19CD3Study designProliferation assaysSelectin inductionGrowth factorFunctional studies
1999
Human single-chain Fv immunoconjugates targeted to a melanoma-associated chondroitin sulfate proteoglycan mediate specific lysis of human melanoma cells by natural killer cells and complement
Wang B, Chen Y, Ayalon O, Bender J, Garen A. Human single-chain Fv immunoconjugates targeted to a melanoma-associated chondroitin sulfate proteoglycan mediate specific lysis of human melanoma cells by natural killer cells and complement. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 1627-1632. PMID: 9990075, PMCID: PMC15540, DOI: 10.1073/pnas.96.4.1627.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineCells, CulturedCHO CellsChondroitin Sulfate ProteoglycansChromatography, LiquidComplement System ProteinsCricetinaeCytotoxicity, ImmunologicDrosophila melanogasterEndothelium, VascularHumansImmunoconjugatesImmunoglobulin FragmentsImmunoglobulin GImmunoglobulin Variable RegionKiller Cells, NaturalMass SpectrometryMelanomaMolecular Sequence DataRestriction MappingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTransfectionTumor Cells, CulturedConceptsHuman melanoma cellsNatural killer cellsNK cellsMelanoma cellsKiller cellsChondroitin sulfate proteoglycanCytolytic responsesMost human melanoma cellsTumor cellsVaccinated melanoma patientsCultured human melanoma cellsCytolytic immune responseFusion phage librarySpecific cytolytic responseMelanoma-associated chondroitin sulfate proteoglycanHuman single-chain FvSulfate proteoglycanMelanoma patientsSpecific lysisImmune responseMelanoma tumorsComplement cascadeTargeted tumor cellsImmunoconjugatesHuman IgG1
1998
Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion.
Collinge M, Pardi R, Bender J. Class II transactivator-independent endothelial cell MHC class II gene activation induced by lymphocyte adhesion. The Journal Of Immunology 1998, 161: 1589-93. PMID: 9712019, DOI: 10.4049/jimmunol.161.4.1589.Peer-Reviewed Original ResearchConceptsClass II transactivatorNK cellsIFN-gamma-dependent mechanismIFN-gamma-independent mannerLymphocyte adhesionMHC class II expressionHLA-DR alpha mRNAHLA-DR expressionMHC class II moleculesNK cell activationAllogeneic endothelial cellsClass II expressionIFN-gamma-induced MHC class II expressionClass II moleculesAlpha mRNA expressionCell linesHLA-DRIFN-gammaCell activationMRNA expressionEndothelial cellsAlpha mRNACellsPromoter constructsActivationInduction of transporter associated with antigen processing by interferon γ confers endothelial cell cytoprotection against natural killer-mediated lysis
Ayalon O, Hughes E, Cresswell P, Lee J, O’Donnell L, Pardi R, Bender J. Induction of transporter associated with antigen processing by interferon γ confers endothelial cell cytoprotection against natural killer-mediated lysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2435-2440. PMID: 9482903, PMCID: PMC19366, DOI: 10.1073/pnas.95.5.2435.Peer-Reviewed Original ResearchConceptsMHC IInduction of transportersEndothelial cellsNK-resistant cell linesNatural killer-mediated lysisMajor histocompatibility complex INK inhibitory receptorsNK effector cellsPretreatment of ECB-lymphoblastoid cell linesEC sensitivityDermal microvascular endothelial cellsMicrovascular endothelial cellsCell linesHuman umbilical vein endothelial cellsExpression/functionUmbilical vein endothelial cellsAdenoviral-mediated transductionVascular vulnerabilityVein endothelial cellsNK assaysEffector cellsEndothelial activationInhibitory receptorsDeficient targets
1996
Overlapping recognition of xenogeneic carbohydrate ligands by human natural killer lymphocytes and natural antibodies.
Inverardi L, Clissi B, Stolzer A, Bender J, Pardi R. Overlapping recognition of xenogeneic carbohydrate ligands by human natural killer lymphocytes and natural antibodies. Transplantation Proceedings 1996, 28: 552. PMID: 8623263.Peer-Reviewed Original Research
1995
Contact-dependent endothelial class II HLA gene activation induced by NK cells is mediated by IFN-gamma-dependent and -independent mechanisms.
Watson C, Petzelbauer P, Zhou J, Pardi R, Bender J. Contact-dependent endothelial class II HLA gene activation induced by NK cells is mediated by IFN-gamma-dependent and -independent mechanisms. The Journal Of Immunology 1995, 154: 3222-33. PMID: 7897208, DOI: 10.4049/jimmunol.154.7.3222.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, B-LymphocyteBase SequenceBlotting, NorthernCell AdhesionCells, CulturedEndothelium, VascularGene Expression RegulationHistocompatibility Antigens Class IIHLA-DR AntigensHumansInterferon-gammaKiller Cells, NaturalMolecular Sequence DataOrgan Culture TechniquesPromoter Regions, GeneticSkinTranscriptional ActivationTransfectionConceptsNK cellsNK lymphocytesEndothelial cellsIFN-gammaMHC class II AgIFN-gamma dependenceT cell recruitmentClass II HLAClass II expressionHLA-DR inductionClass II AgT cell proliferationMembrane expressionTrans-well experimentsReceptor AbEndothelial activationImmune amplificationCell recruitmentMicrovessel endotheliumHuman IFN-gammaPromoter constructsClonal expansionCoculture modelCell proliferationChinese hamster ovary cellsLymphocyte adhesion-dependent calcium signaling in human endothelial cells.
Pfau S, Leitenberg D, Rinder H, Smith BR, Pardi R, Bender JR. Lymphocyte adhesion-dependent calcium signaling in human endothelial cells. Journal Of Cell Biology 1995, 128: 969-978. PMID: 7533170, PMCID: PMC2120392, DOI: 10.1083/jcb.128.5.969.Peer-Reviewed Original ResearchConceptsEC Ca2Endothelial cellsEC alterationsLymphocyte-endothelial interactionsNatural killer cellsImmune effector cellsTumor necrosis factorHuman umbilical vein endothelial cellsCytosolic free calcium changesUmbilical vein endothelial cellsVascular endothelial cellsHuman umbilical vein ECsAllogeneic CD3Lymphocyte subsetsVein endothelial cellsHuman endothelial cellsNK cellsEffector cellsKiller cellsVascular toneInflammatory responseNecrosis factorSecond messenger pathwaysExtracellular influxCytokine activation
1992
Early recognition of a discordant xenogeneic organ by human circulating lymphocytes.
Inverardi L, Samaja M, Motterlini R, Mangili F, Bender J, Pardi R. Early recognition of a discordant xenogeneic organ by human circulating lymphocytes. The Journal Of Immunology 1992, 149: 1416-23. PMID: 1386865, DOI: 10.4049/jimmunol.149.4.1416.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, DifferentiationAntigens, Differentiation, T-LymphocyteCD3 ComplexCell AdhesionCytotoxicity, ImmunologicEndothelium, VascularHeart TransplantationHumansImmunity, CellularKiller Cells, NaturalLymphocyte Function-Associated Antigen-1Lymphocyte SubsetsMyocardiumRatsReceptors, Antigen, T-CellReceptors, FcReceptors, IgGTransplantation, HeterologousConceptsNK cellsXenogeneic organsCell-mediated immune mechanismsHuman lymphocytesAntibody-independent pathwayLymphocyte phenotypic analysisCell-mediated mechanismsHuman NK cellsNK cell adhesionPretreatment of lymphocytesAnti-CD18 antibodiesDiscordant xenograft rejectionHuman circulating lymphocytesEx vivo modelRat endothelial cellsCD3-CD16Vascular resistanceLymphocyte subsetsXenograft rejectionCirculating lymphocytesCoronary systemEarly recognitionImmune mechanismsT lymphocytesCell sequestrationCellular early immune recognition of xenogeneic vascular endothelium.
Inverardi L, Samaja M, Marelli F, Bender J, Pardi R. Cellular early immune recognition of xenogeneic vascular endothelium. Transplantation Proceedings 1992, 24: 459-61. PMID: 1566390.Peer-Reviewed Original Research
1990
T-cell receptor-negative natural killer cells display antigen-specific cytotoxicity for microvascular endothelial cells.
Bender J, Pardi R, Engleman E. T-cell receptor-negative natural killer cells display antigen-specific cytotoxicity for microvascular endothelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 6949-6953. PMID: 2402485, PMCID: PMC54659, DOI: 10.1073/pnas.87.18.6949.Peer-Reviewed Original ResearchConceptsMicrovascular endothelial cellsEndothelial cellsNK cellsNatural killer cell targetsNatural killer cellsAllogeneic endothelial cellsAntigen-specific cytotoxicityNK cell linesCD3/T cell receptorLevel of adherenceEC linesHuman microvascular endothelial cellsT cell receptorOrgan allograftsGamma chain geneKiller cellsLymphoid cellsSpecific cytotoxicityCell targetsNegative cellsCell linesHuman erythroleukemia cellsSelective cytotoxicityTi moleculesCytotoxicityNatural killer lines and clones with apparent antigen specificity.
Suzuki N, Bianchi E, Bass H, Suzuki T, Bender J, Pardi R, Brenner C, Larrick J, Engleman E. Natural killer lines and clones with apparent antigen specificity. Journal Of Experimental Medicine 1990, 172: 457-462. PMID: 2142719, PMCID: PMC2188332, DOI: 10.1084/jem.172.2.457.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigen-Antibody ComplexAntigens, CDAntigens, DifferentiationAntigens, Differentiation, T-LymphocyteCD3 ComplexCell LineCells, CulturedClone CellsCytotoxicity, ImmunologicFlow CytometryHumansKiller Cells, NaturalMacromolecular SubstancesReceptors, Antigen, T-CellReceptors, FcReceptors, IgGConceptsLymphoblastoid cell linesAllogeneic lymphoblastoid cell linesTCR alpha/betaClass II MHC antigensIL-2-containing mediumPotent cytolytic activityNK cellsAllogeneic targetsCD3 lymphocytesMHC antigensCytolytic activityAntigen specificityTCR-beta transcriptsCD3Class ICD16CD3 epsilonSelective killingNorthern blot analysisBlot analysisCell linesK562 cellsK562 erythroleukemia cellsChain transcriptsMAbs
1987
Lymphocyte subsets differentially induce class II human leukocyte antigens on allogeneic microvascular endothelial cells.
Pardi R, Bender J, Engleman E. Lymphocyte subsets differentially induce class II human leukocyte antigens on allogeneic microvascular endothelial cells. The Journal Of Immunology 1987, 139: 2585-92. PMID: 3116086, DOI: 10.4049/jimmunol.139.8.2585.Peer-Reviewed Original ResearchConceptsClass II human leukocyteMicrovascular endothelial cellsEndothelial cellsT cellsAllogeneic T cell proliferationMajor histocompatibility complex class IIHuman leukocytesHistocompatibility complex class IILymphocyte-EC adhesionT-cell surface antigensNatural killer cellsT cell proliferationHost immune responseHuman microvascular endothelial cellsCell surface antigensAcute rejectionAntilymphocyte antibodiesGraft alloantigensLymphocyte subsetsAllogeneic lymphocytesAllograft rejectionLymphocyte subpopulationsKiller cellsAutologous monocytesCD18 antibodyPhenotypic and functional characterization of lymphocytes that bind human microvascular endothelial cells in vitro. Evidence for preferential binding of natural killer cells.
Bender J, Pardi R, Karasek M, Engleman E. Phenotypic and functional characterization of lymphocytes that bind human microvascular endothelial cells in vitro. Evidence for preferential binding of natural killer cells. Journal Of Clinical Investigation 1987, 79: 1679-1688. PMID: 3495552, PMCID: PMC424501, DOI: 10.1172/jci113007.Peer-Reviewed Original ResearchConceptsNatural killer cellsMicrovascular endothelial cellsEndothelial cellsKiller cellsE-RFCRosette forming lymphocytesRecombinant interleukin-1Human microvascular endothelial cellsReceptor-ligand interactionsLymphocyte subsetsNK effectorsNK targetsGraft rejectionNK cellsLeu-11Lymphocyte fractionInterleukin-1Microvascular endotheliumSpecific receptor-ligand interactionsAllograftsLymphocytesEC monolayersCritical targetCellsQuantitative adherence