2001
Ex vivo evaluation of PBMNCs collected with a new cell separator
Snyder E, O'Donnell L, Dengler T, Pomper G, Velleca M, Dincecco D, Baril L, Min K, Gudino M, Bender J. Ex vivo evaluation of PBMNCs collected with a new cell separator. Transfusion 2001, 41: 940-949. PMID: 11452164, DOI: 10.1046/j.1537-2995.2001.41070940.x.Peer-Reviewed Original ResearchMeSH KeywordsBlood DonorsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell DivisionCell SeparationCytotoxicity Tests, ImmunologicEndothelium, VascularE-SelectinHematopoietic Stem Cell TransplantationHumansInfant, NewbornKiller Cells, NaturalLeukapheresisLeukocytes, MononuclearTime FactorsUmbilical VeinsConceptsDendritic cellsNK cellsCell separatorHealthy volunteer blood donorsMNC subsetsEndothelial cellsVolunteer blood donorsB cell subsetsCell surface markersAmicus SeparatorNK cytotoxicityBlood donorsSelectin expressionAppropriate cell surface markersCD8CD4Ex vivoSurface markersCD19CD3Study designProliferation assaysSelectin inductionGrowth factorFunctional studies
2000
CD28 and LFA‐1 contribute to cyclosporin A‐resistant T cell growth by stabilizing the IL‐2 mRNA through distinct signaling pathways
Geginat J, Clissi B, Moro M, Dellabona P, Bender J, Pardi R. CD28 and LFA‐1 contribute to cyclosporin A‐resistant T cell growth by stabilizing the IL‐2 mRNA through distinct signaling pathways. European Journal Of Immunology 2000, 30: 1136-1144. PMID: 10760803, DOI: 10.1002/(sici)1521-4141(200004)30:4<1136::aid-immu1136>3.0.co;2-3.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDB7-2 AntigenCalcineurinCD28 AntigensCells, CulturedCyclosporineCytoskeletonDendritic CellsDNA-Binding ProteinsDrug SynergismHumansIntercellular Adhesion Molecule-1Interleukin-2Lymphocyte ActivationLymphocyte Function-Associated Antigen-1Membrane GlycoproteinsMitogen-Activated Protein KinasesNFATC Transcription FactorsNF-kappa BNuclear ProteinsPromoter Regions, GeneticProtein BindingRNA StabilityRNA, MessengerSignal TransductionSuperantigensT-LymphocytesTranscription FactorsConceptsIL-2 mRNALFA-1ICAM-1IL-2 dependentT cell proliferationSubsequent T cell proliferationCostimulatory molecule CD28TCR-induced proliferationSignaling pathwaysT cell growthIL-2 transcriptsGraft rejectionDendritic cellsIL-2Clinical transplantationT lymphocytesMolecule CD28Primary T lymphocytesNF-kappaBCD28Distinct signaling pathwaysLower transcriptional rateDifferent signaling pathwaysProtein kinase activationCell proliferation