2011
Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complex
Shi L, Kümmel D, Coleman J, Melia TJ, Giraudo CG. Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complex. Molecular Biology Of The Cell 2011, 22: 4150-4160. PMID: 21900493, PMCID: PMC3204075, DOI: 10.1091/mbc.e11-02-0150.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalorimetryCell MembraneHeLa CellsHuman Growth HormoneHumansLiposomesMiceMicroscopy, FluorescenceMolecular ChaperonesMunc18 ProteinsNeuroendocrine CellsPC12 CellsProtein BindingProtein Interaction Domains and MotifsProtein TransportRatsRecombinant ProteinsSNARE ProteinsSyntaxin 1ThermodynamicsTitrimetryConceptsSoluble N-ethylmaleimide-sensitive factor attachment protein receptorsSyntaxin 1AMunc18-1N-ethylmaleimide-sensitive factor attachment protein receptorsSec1/Munc18 (SM) proteinsFactor attachment protein receptorsCentral cavitySNARE complex assemblyIntracellular membrane traffickingAttachment protein receptorsMunc18-1 mutantsLiposome fusionPrecise molecular mechanismsMunc18 proteinsMembrane traffickingSNARE complexChaperone functionH3 domainDistinct binding modesMembrane fusionMolecular mechanismsProtein receptorsDual functionNeuroendocrine cellsBinding modes
2010
Phosphatidylinositol 4-Phosphate Controls Both Membrane Recruitment and a Regulatory Switch of the Rab GEF Sec2p
Mizuno-Yamasaki E, Medkova M, Coleman J, Novick P. Phosphatidylinositol 4-Phosphate Controls Both Membrane Recruitment and a Regulatory Switch of the Rab GEF Sec2p. Developmental Cell 2010, 18: 828-840. PMID: 20493815, PMCID: PMC2877039, DOI: 10.1016/j.devcel.2010.03.016.Peer-Reviewed Original ResearchConceptsPI4P levelsRab GTPase Sec4pMembrane recruitmentRegulatory switchExchange factorSecretory pathwayVesicle maturationSec2pSec15pSecretory vesiclesPositive feedback loopYpt32pPhosphatidylinositol 4Vesicles formSec4pVesiclesSecretory sitesFeedback loopRecruitment cascadeCascadeRecruitmentPI4PRabPhosphatidylinositolGuanine