2023
Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy
Thoduvayil S, Weerakkody J, Sundaram R, Topper M, Bera M, Coleman J, Li X, Mariappan M, Ramakrishnan S. Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy. Cell Calcium 2023, 113: 102766. PMID: 37295201, PMCID: PMC10450995, DOI: 10.1016/j.ceca.2023.102766.Peer-Reviewed Original ResearchConceptsCellular pathwaysSecretion dynamicsSmall molecule screeningLuciferase reporter systemInsulin secretion dynamicsHigh-throughput compoundInsulin secretionHigh-throughput quantificationReporter systemGenetic studiesLive cellsDistinct rolesInsulin therapyGlucose-stimulated insulin secretionSmall moleculesEffective insulin therapyPathwaySecretion
2006
The rab Exchange Factor Sec2p Reversibly Associates with the Exocyst
Medkova M, France Y, Coleman J, Novick P. The rab Exchange Factor Sec2p Reversibly Associates with the Exocyst. Molecular Biology Of The Cell 2006, 17: 2757-2769. PMID: 16611746, PMCID: PMC1474791, DOI: 10.1091/mbc.e05-10-0917.Peer-Reviewed Original ResearchConceptsSecretory vesiclesExchange factor Sec2pTemperature-sensitive growthC-terminal halfExocyst complexExocytic sitesRab GTPaseExchange factorMutant resultsMutant correlatesRecycling pathwaySec2pExocystNucleotide exchangePlasma membraneSec4pSec15pVesiclesMislocalizationEffectorsPolarized transportAssociatesGTPaseExocytosisPathwayThe polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit
France Y, Boyd C, Coleman J, Novick P. The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit. Journal Of Cell Science 2006, 119: 876-888. PMID: 16478783, DOI: 10.1242/jcs.02849.Peer-Reviewed Original ResearchConceptsBud growthSrc homology 3 domainTwo-hybrid studiesFirst Src homology 3 domainDirect physical interactionGreen fluorescent proteinExocyst complexGolgi traffickingSec15pSecretory pathwaySpatial regulationBem1pSecretory machineryMaster regulatorFluorescent proteinNew budsMachineryExocystSec4pPhysical interactionSubunitsCase of cellsProteinPathwayCrucial role