2008
ATP8B1 Deficiency Disrupts the Bile Canalicular Membrane Bilayer Structure in Hepatocytes, But FXR Expression and Activity Are Maintained
Cai S, Gautam S, Nguyen T, Soroka CJ, Rahner C, Boyer JL. ATP8B1 Deficiency Disrupts the Bile Canalicular Membrane Bilayer Structure in Hepatocytes, But FXR Expression and Activity Are Maintained. Gastroenterology 2008, 136: 1060-1069.e4. PMID: 19027009, PMCID: PMC3439851, DOI: 10.1053/j.gastro.2008.10.025.Peer-Reviewed Original ResearchMeSH Keywords4-Chloro-7-nitrobenzofurazanAdenosine TriphosphatasesAnimalsATP Binding Cassette Transporter, Subfamily B, Member 11ATP-Binding Cassette TransportersBile CanaliculiCaco-2 CellsChenodeoxycholic AcidDNA-Binding ProteinsGastrointestinal AgentsGene ExpressionHepatocytesHumansMultidrug Resistance-Associated Protein 2PhosphatidylserinesPhospholipid Transfer ProteinsRatsReceptors, Cytoplasmic and NuclearRNA, Small InterferingTranscription FactorsTransfectionConceptsAminophospholipid flippaseMessenger RNAMembrane bilayer structureCanalicular membraneFarnesoid X receptorRat hepatocytesSmall heterodimer partnerMembrane transportersNBD-phosphatidylserineHeterodimer partnerDeficiency disruptsLuminal accumulationMembrane disruptionRNAConflicting hypothesesRat cellsFlippaseProtein levelsProtein expressionX receptorExpressionBSEP functionATP8B1CellsMembrane
1999
Canalicular Export Pumps Traffic with Polymeric Immunoglobulin A Receptor on the Same Microtubule-associated Vesicle in Rat Liver*
Soroka C, Pate M, Boyer J. Canalicular Export Pumps Traffic with Polymeric Immunoglobulin A Receptor on the Same Microtubule-associated Vesicle in Rat Liver*. Journal Of Biological Chemistry 1999, 274: 26416-26424. PMID: 10473600, DOI: 10.1074/jbc.274.37.26416.Peer-Reviewed Original ResearchConceptsApical membrane proteinsMembrane proteinsMicrotubule-associated vesiclesSite of functionMembrane transport proteinsMembrane-bound vesiclesNon-hydrolyzable nucleotidesReceptor trafficMicrotubule motorsTransport proteinsIntermediate chainEssential pathwayPolymeric immunoglobulinsSame vesiclesTranscytotic vesiclesMultidrug resistance protein 2Protein 2Immunoelectron microscopyVesiclesMicrotubulesProteinVesicular transcytosisPathwayRat hepatocytesCanalicular export
1995
Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets
Boyer J, Soroka C. Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets. Gastroenterology 1995, 109: 1600-1611. PMID: 7557144, DOI: 10.1016/0016-5085(95)90649-5.Peer-Reviewed Original ResearchConceptsInsertion of vesiclesTranslocation of vesiclesMembrane transport activityVesicle trafficApical domainIsolated rat hepatocyte coupletsAcid transportersTransport activityNocodazoleRat hepatocyte coupletsVesiclesIndependent mechanismsEpithelial cellsBile acid transporterSite of residenceHepatocyte coupletsTransportersCanalicular membraneRat hepatocytesImmunocytochemical localizationCyclic monophosphateAdenosine triphosphataseBile excretory function
1994
Expression and characterization of a functional rat liver Na+ bile acid cotransport system in COS-7 cells
Boyer J, Ng O, Ananthanarayanan M, Hofmann A, Schteingart C, Hagenbuch B, Stieger B, Meier P. Expression and characterization of a functional rat liver Na+ bile acid cotransport system in COS-7 cells. American Journal Of Physiology 1994, 266: g382-g387. PMID: 8166278, DOI: 10.1152/ajpgi.1994.266.3.g382.Peer-Reviewed Original ResearchConceptsCOS-7 cellsCotransport systemBile acid transporterUrsodeoxycholic acidTaurochenodeoxycholic acidAcid cotransport systemTauroursodeoxycholic acidBile acidsRat hepatocytesAbsence of sodiumChenodeoxycholic acidCOS cellsEmpty plasmidPMAMneo vectorCholic acidBile acid cotransporterMicroM bilirubinRat liverEvidence of expressionCell linesAcid transportersPosttranslational factorsProgressive uptakeCotransporterAcid cotransporterRegulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes.
Benedetti A, Strazzabosco M, Ng O, Boyer J. Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 792-796. PMID: 8290601, PMCID: PMC43035, DOI: 10.1073/pnas.91.2.792.Peer-Reviewed Original ResearchConceptsApical targetingExchange activityRegulation of activityMembrane localizationCl-/HCO3Dibutyryl cAMPProtein kinase C agonistsRat hepatocytesProtein kinase C agonist phorbolEcto-ATPaseConfocal microscopyCanalicular domainC agonistsProteinVesiclesTargetingCanalicular localizationLocalizationCAMPHepatocyte coupletsHepatocytesActivityRegulationPhorbolColchicine
1993
Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL
Bruck R, Benedetti A, Strazzabosco M, Boyer J. Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL. American Journal Of Physiology 1993, 265: g347-g353. PMID: 8368316, DOI: 10.1152/ajpgi.1993.265.2.g347.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAlkaliesAnimalsAnionsBariumBicarbonatesBileBiological TransportColchicineExocytosisHorseradish PeroxidaseHydrogen-Ion ConcentrationIn Vitro TechniquesIntracellular MembranesLiverMalePotassium ChannelsRatsRats, Sprague-DawleyConceptsBiliary HCO3- excretionBile flowHCO3- excretionIntracellular alkalinizationHCO3- exchangerAcute alkaline loadVesicle-mediated exocytosisBiliary excretionTransient increasePH recoveryAlkaline loadMembrane depolarizationRat liverChannel blocker BaCl2Intact liverLiverAlkalinizationMM NH4ClHCO3- concentrationDisulfonic acidDIDSRat hepatocytesColchicinePretreatmentExocytosisEffects of tauroursodeoxycholic acid on cytosolic Ca2+ signals in isolated rat hepatocytes
Beuers U, Nathanson M, Boyer J. Effects of tauroursodeoxycholic acid on cytosolic Ca2+ signals in isolated rat hepatocytes. Gastroenterology 1993, 104: 604-612. PMID: 8425704, DOI: 10.1016/0016-5085(93)90433-d.Peer-Reviewed Original ResearchConceptsEffect of TUDCATauroursodeoxycholic acidAdministration of TUDCAIP3-independent mechanismCytosolic free calciumIP3-sensitive Ca2Trihydroxy bile acidsCholestatic disordersHepatocyte secretionTreatment periodExtracellular Ca2Bile acidsTaurolithocholic acidMumol/Rat hepatocytesFree calciumBasal levelsTransient increaseCytosolic Ca2Levels of inositolFree mediumPhysiological concentrationsIsolated rat hepatocytesHepatocytesFourfold increase
1991
Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH
Benedetti A, Strazzabosco M, Corasanti J, Haddad P, Graf J, Boyer J. Cl(-)-HCO3- exchanger in isolated rat hepatocytes: role in regulation of intracellular pH. American Journal Of Physiology 1991, 261: g512-g522. PMID: 1887897, DOI: 10.1152/ajpgi.1991.261.3.g512.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmmoniaAnimalsBariumBicarbonatesCarrier ProteinsCells, CulturedChloride-Bicarbonate AntiportersChloridesHydrogen-Ion ConcentrationIntracellular FluidKineticsLiverMaleRatsRats, Inbred StrainsConceptsAlkaline loadPH-sensitive dye BCECFMM H2DIDSPHi recoveryRegulation of intracellularRat hepatocyte monolayersAcid extrudersMM DIDSRat hepatocytesElectrophysiological techniquesDye BCECFHCO3- exchangerIsolated rat hepatocytesConstant pHoIntracellularMembrane potentialHepatocyte monolayersHepatocytesAcid loadingRole of chloride ions in liver cell volume regulation
Haddad P, Beck J, Boyer J, Graf J. Role of chloride ions in liver cell volume regulation. American Journal Of Physiology 1991, 261: g340-g348. PMID: 1872402, DOI: 10.1152/ajpgi.1991.261.2.g340.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseM omegaLiver cellsSingle isolated rat hepatocytesBiphasic responseHypotonic stressInitial hyperpolarizationPl/minIsolated rat hepatocytesGradual depolarizationCell volume regulationExternal ClRole of C1Hypotonic swellingVolume regulationMembrane potentialFacilitated releaseRat hepatocytesCellsMinVolume decreaseCell volumeInitial volumeInitial rateLiver
1990
Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation
Corasanti J, Gleeson D, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. I. Ionic mechanisms of cell volume regulation. American Journal Of Physiology 1990, 258: g290-g298. PMID: 2305895, DOI: 10.1152/ajpgi.1990.258.2.g290.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAmilorideAnimalsBariumBicarbonatesBumetanideCells, CulturedChloridesHypertonic SolutionsHypotonic SolutionsKineticsLiverMaleOsmolar ConcentrationPotassium ChlorideQuinineRatsRats, Inbred StrainsSodium ChlorideEffects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH
Gleeson D, Corasanti J, Boyer J. Effects of osmotic stresses on isolated rat hepatocytes. II. Modulation of intracellular pH. American Journal Of Physiology 1990, 258: g299-g307. PMID: 2305896, DOI: 10.1152/ajpgi.1990.258.2.g299.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAnimalsBicarbonatesBuffersCells, CulturedCholineHydrogen-Ion ConcentrationHypertonic SolutionsHypotonic SolutionsIntracellular FluidKineticsLiverMaleOsmolar ConcentrationRatsRats, Inbred StrainsSodiumConceptsRegulatory volume decreaseAbsence of HCO3Presence of HCO3Acid-base transport systemsModulation of intracellularRat hepatocytesVolume regulationAcute withdrawalMM amilorideGreater intracellularHCO3- exchangeAcid effluxIsotonic mediumIsolated rat hepatocytesSame maneuverCell volume regulationHepatocytesNet acid effluxAmilorideSubconfluent monolayersDisulfonic acidIntracellularVolume decreaseHypotonic mediumHypertonic medium
1989
Bicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport.
Gleeson D, Smith N, Boyer J. Bicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport. Journal Of Clinical Investigation 1989, 84: 312-321. PMID: 2544626, PMCID: PMC303984, DOI: 10.1172/jci114156.Peer-Reviewed Original ResearchConceptsDisulfonic acid stilbeneHCO3- cotransportIntracellular acid loadHCO3- thanIntracellular pH regulatory mechanismsPH regulatory mechanismsBaseline pHiMonolayer cell culture systemRat hepatocytesAcid loadIntracellular ClCl-/HCO3AmilorideCell culture systemAnion transport inhibitorAbsence of bicarbonateHCO3- transportImportant regulatorHepatocytesPH-sensitive dyeCarboxy-fluoresceinTransport inhibitorsCulture systemRegulatory mechanismsPhiInward-rectifying potassium channels in rat hepatocytes
Henderson R, Graf J, Boyer J. Inward-rectifying potassium channels in rat hepatocytes. American Journal Of Physiology 1989, 256: g1028-g1035. PMID: 2735409, DOI: 10.1152/ajpgi.1989.256.6.g1028.Peer-Reviewed Original ResearchConceptsRenal epithelial cellsRat hepatocytesWhole-cell conductanceWhole-cell experimentsSingle-channel recordingsEpithelial cellsPotassium channelsHigh intracellularFunctional heterogeneityOpen probabilityIsolated rat hepatocytesCellsWhole-cell modeInward rectificationMarked similarityStrong inward rectificationCell conductanceHepatocytesConductanceCalcium dependencePatch-clamp techniqueCurrent-voltage relationshipInward conductanceSpeciesCell experiments
1988
Characteristics of L-alanine uptake in freshly isolated hepatocytes of elasmobranch Raja erinacea
Ballatori N, Boyer J. Characteristics of L-alanine uptake in freshly isolated hepatocytes of elasmobranch Raja erinacea. American Journal Of Physiology 1988, 254: r801-r808. PMID: 3364608, DOI: 10.1152/ajpregu.1988.254.5.r801.Peer-Reviewed Original ResearchConceptsNeutral amino acid transport systemElasmobranch Raja erinaceaMajor gluconeogenic precursorMM alanineSodium-dependent systemAmino acid transport systemsRaja erinaceaHepatic uptakeHepatic concentrationsAcid transport systemL-alanine uptakeAminotransferase activityTumor cellsSpecific transport mechanismsMeAIB transportGluconeogenic precursorsSaturable mechanismSkate hepatocytesHepatocytesAlanine metabolismGlucose releaseMeAIBHepatocyte suspensionsRat hepatocytesUptake
1985
The effect of ethanol and calcium on fluid state of plasma membranes of rat hepatocytes
Mills P, Meier P, Boyer J, Gordon E. The effect of ethanol and calcium on fluid state of plasma membranes of rat hepatocytes. Alcohol 1985, 2: 153-156. PMID: 4015830, DOI: 10.1016/0741-8329(85)90034-5.Peer-Reviewed Original ResearchConceptsMembrane protein functionProtein functionMembrane fluidityCanalicular plasma membrane vesiclesPlasma membrane vesiclesOverall cellular activityMembrane lipid fluidityPlasma membraneMembrane preparationsMembrane vesiclesCellular activitiesImportant regulatorLipid fluidityMembrane perturbantsFluorescence polarizationRat hepatocytesFluidityBlLPMRegulatorUptakeUptake of taurocholateVesiclesMillipore filtration technique