2023
Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection
Agidigbi T, Kwon H, Knight J, Zhao D, Lee F, Oh I. Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection. Frontiers In Cellular And Infection Microbiology 2023, 13: 1198115. PMID: 37434783, PMCID: PMC10332306, DOI: 10.3389/fcimb.2023.1198115.Peer-Reviewed Original ResearchConceptsDiabetic foot ulcersPeripheral blood mononuclear cellsHost immune responseActive infectionImmune responseDiabetic foot ulcer infectionsInfected diabetic foot ulcersFoot ulcer infectionsPatients 8 weeksIntravenous antibiotic therapyBlood mononuclear cellsWound healing statusDFU infectionsPBMC expressionSalvage therapyUlcer infectionDifferent time pointsAntibiotic therapyMajor complicationsSurgical treatmentFoot ulcersMononuclear cellsPotential intervention optionsSpecies-specific infectionTreatment response
2019
Evidence of differential microbiomes in healing versus non‐healing diabetic foot ulcers prior to and following foot salvage therapy
MacDonald A, Brodell JD, Daiss JL, Schwarz EM, Oh I. Evidence of differential microbiomes in healing versus non‐healing diabetic foot ulcers prior to and following foot salvage therapy. Journal Of Orthopaedic Research® 2019, 37: 1596-1603. PMID: 30908702, PMCID: PMC6659747, DOI: 10.1002/jor.24279.Peer-Reviewed Original ResearchConceptsSalvage therapyQuantitative polymerase chain reactionClinical outcomesClinical toolErythrocyte sedimentation rateFurther surgical interventionC-reactive proteinLower extremity amputationDiabetic foot ulcersEventual clinical outcomeImportant prognostic informationImportant clinical toolDFU patientsFoot salvageFoot ulcersDiabetic footExtremity amputationSurgical interventionPatient counselingAntibiotic treatmentPolymerase chain reactionPrognostic informationClinical signsMicrobiome analysisClinical significance
2018
Tracking Anti-Staphylococcus aureus Antibodies Produced In Vivo and Ex Vivo during Foot Salvage Therapy for Diabetic Foot Infections Reveals Prognostic Insights and Evidence of Diversified Humoral Immunity
Oh I, Muthukrishnan G, Ninomiya MJ, Brodell JD, Smith BL, Lee CC, Gill SR, Beck CA, Schwarz EM, Daiss JL. Tracking Anti-Staphylococcus aureus Antibodies Produced In Vivo and Ex Vivo during Foot Salvage Therapy for Diabetic Foot Infections Reveals Prognostic Insights and Evidence of Diversified Humoral Immunity. Infection And Immunity 2018, 86: 10.1128/iai.00629-18. PMID: 30275008, PMCID: PMC6246899, DOI: 10.1128/iai.00629-18.Peer-Reviewed Original ResearchConceptsDiabetic foot infectionsIgG levelsDFI patientsSalvage therapyFoot infectionsCultured peripheral blood mononuclear cellsPeripheral blood mononuclear cellsBlood mononuclear cellsSimilar diagnostic potentialAggressive treatmentAntibody titersHumoral immunityMononuclear cellsPrognostic insightsPrognostic potentialCommon pathogensEtiologic agentMultivariate analysisClinical decisionEx vivoMultiplex immunoassayWound samplesInfectionSerumAureus antibody
2017
Clinical Application of Immunoassay for Identification of Staphylococcus aureus and Monitoring Treatment Response in Diabetic Foot Infection
Oh I, Smith B, Grier A, Gill S, Daiss J. Clinical Application of Immunoassay for Identification of Staphylococcus aureus and Monitoring Treatment Response in Diabetic Foot Infection. Foot & Ankle Orthopaedics 2017, 2: 2473011417s000065. DOI: 10.1177/2473011417s000065.Peer-Reviewed Original ResearchDiabetic foot infectionsFoot infectionsSA infectionsAntibiotic treatmentTreatment responseStaphylococcus aureusPathogenic activityAdequate prognostic informationNonspecific inflammatory markersIntravenous antibiotic treatmentWhole bloodMonitoring Treatment ResponseSigns of infectionStandard cultureAntibody-secreting cellsMicrobiome analysisFoot salvageSalvage therapySalvage treatmentSpecific diagnostic toolsInflammatory markersDiabetic patientsCounseling patientsClinical symptomsWeeks 0