2020
Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis
Li J, Huynh L, Cornwell WD, Tang MS, Simborio H, Huang J, Kosmider B, Rogers TJ, Zhao H, Steinberg MB, Thu Thi Le L, Zhang L, Pham K, Liu C, Wang H. Electronic Cigarettes Induce Mitochondrial DNA Damage and Trigger TLR9 (Toll-Like Receptor 9)-Mediated Atherosclerosis. Arteriosclerosis Thrombosis And Vascular Biology 2020, 41: 839-853. PMID: 33380174, PMCID: PMC8608030, DOI: 10.1161/atvbaha.120.315556.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaAtherosclerosisDisease Models, AnimalDNA DamageDNA, MitochondrialE-Cigarette VaporFemaleHumansInflammationInflammation MediatorsMacrophagesMaleMiceMice, Inbred C57BLMice, Knockout, ApoEMiddle AgedMitochondriaRAW 264.7 CellsSignal TransductionSmokersToll-Like Receptor 9VapingConceptsECV exposureTLR9 expressionInflammatory cytokinesClassical monocytesTLR9 activationAtherosclerotic plaquesEight-week-old ApoEUpregulation of TLR9Expression of TLR9Atherosclerotic lesion developmentOil Red O stainingToll-like receptorsDays/weekE-cig exposureMonocytes/macrophagesNormal laboratory dietRed O stainingPotential pharmacological targetElectronic cigarette useHours/dayProinflammatory cytokinesCig vaporPlasma levelsTLR9 antagonistTLR9 inhibitor
2011
Giant cell aortitis of the ascending aorta without signs or symptoms of systemic vasculitis is associated with elevated risk of distal aortic events
Wang H, Smith R, Spooner A, Isselbacher E, Cambria R, MacGillivray T, Stone J, Stone J. Giant cell aortitis of the ascending aorta without signs or symptoms of systemic vasculitis is associated with elevated risk of distal aortic events. Arthritis & Rheumatism 2011, 64: 317-319. PMID: 21953530, DOI: 10.1002/art.33343.Peer-Reviewed Original Research
1998
Vascular Endothelial Growth Factor Upregulates the Expression of Matrix Metalloproteinases in Vascular Smooth Muscle Cells
Wang H, Keiser J. Vascular Endothelial Growth Factor Upregulates the Expression of Matrix Metalloproteinases in Vascular Smooth Muscle Cells. Circulation Research 1998, 83: 832-840. PMID: 9776730, DOI: 10.1161/01.res.83.8.832.Peer-Reviewed Original ResearchMeSH KeywordsAortaBiocompatible MaterialsCell DivisionCell MovementCells, CulturedCollagenCollagenasesDrug CombinationsEndothelial Growth FactorsEndothelium, VascularEnzyme ActivationEnzyme-Linked Immunosorbent AssayExtracellular MatrixGelsGene Expression Regulation, EnzymologicHumansLamininLymphokinesMatrix Metalloproteinase 1Matrix Metalloproteinase 9Muscle, Smooth, VascularNeovascularization, PhysiologicPhosphorylationProteoglycansProto-Oncogene ProteinsReceptor Protein-Tyrosine KinasesRNA, MessengerVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1Vascular Endothelial Growth FactorsConceptsHuman smooth muscle cellsKinase insert domain-containing receptorSmooth muscle cellsVascular smooth muscle cellsSMC migrationSignal transduction pathwaysVascular endothelial growth factorExtracellular matrix barriersMuscle cellsNorthern blot analysisMajor cell typesEffects of VEGFGrowth factorDomain-containing receptorTransduction pathwaysInsert domain-containing receptorFlt-1Endothelial cellsExpression of Membrane-Type Matrix Metalloproteinase in Rabbit Neointimal Tissue and Its Correlation with Matrix-Metalloproteinase-2 Activation
Wang H, Keiser J. Expression of Membrane-Type Matrix Metalloproteinase in Rabbit Neointimal Tissue and Its Correlation with Matrix-Metalloproteinase-2 Activation. Journal Of Vascular Research 1998, 35: 45-54. PMID: 9482695, DOI: 10.1159/000025564.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAortaBase SequenceBlotting, NorthernCells, CulturedDNA, ComplementaryEnzyme ActivationGelatinasesGene ExpressionHumansMaleMatrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMolecular Sequence DataMuscle, Smooth, VascularPolymerase Chain ReactionRabbitsRNA ProbesRNA, MessengerSequence HomologyConceptsBalloon catheter de-endothelializationMMP-2 activationArterial SMCsTemporal expression patternsMembrane-type matrix metalloproteinasesMembrane-type matrix metalloproteinaseNorthern blot analysisMatrix metalloproteinasesExtracellular matrix degradationRabbit cDNAMammalian cellsMT-MMP-1Matrix metalloproteinase-2 activationMT-MMP-1 expressionExpression patternsVascular neointimal formationSequence analysisMMP-2MMP cDNAPhenotypic alterationsCDNAPeak expressionRNA probesBlot analysisMatrix degradationCollagen biosynthesis by neointimal smooth muscle cells cultured from rabbit aortic explants 15 weeks after de‐endothelialization
WANG H, LI Z, MOORE S, ALAVI M. Collagen biosynthesis by neointimal smooth muscle cells cultured from rabbit aortic explants 15 weeks after de‐endothelialization. International Journal Of Experimental Pathology 1998, 79: 47-53. PMID: 9614349, PMCID: PMC3219429, DOI: 10.1046/j.1365-2613.1998.00048.x.Peer-Reviewed Original ResearchConceptsNeointimal smooth muscle cellsSmooth muscle cellsArterial neointimaCollagen accumulationMuscle cellsConfluent smooth muscle cellsBalloon catheter injuryExtracellular matrix accumulationCatheter injuryDe-endothelializationNormal aortaProcollagen IIIMatrix accumulationAortic neointimaMRNA expression
1997
Expression of syndecan-1 in rabbit neointima following de-endothelialization by a balloon catheter
Wang H, Moore S, Alavi M. Expression of syndecan-1 in rabbit neointima following de-endothelialization by a balloon catheter. Atherosclerosis 1997, 131: 141-147. PMID: 9199266, DOI: 10.1016/s0021-9150(97)06078-4.Peer-Reviewed Original ResearchConceptsSyndecan-1Low density lipoprotein retentionSyndecan-1 expressionArterial injuryBalloon catheterImmunohistochemical stainingEarly atherogenesisNeointimal cellsPositive cellsArterial neointimaRabbit aortaNormal aortaNeointimal tissueRabbit neointimaAortic neointimaNeointimaLipoprotein retentionCell membrane proteoglycansGrowth factorProtein levelsExtracellular matrix componentsNorthern blot analysisAortaBlot analysisInjury
1996
Synthesis of tissue inhibitor of metalloproteinase-1 (TIMP-1) in rabbit aortic neointima after selective de-endothelialization
Wang H, Moore S, Alavi M. Synthesis of tissue inhibitor of metalloproteinase-1 (TIMP-1) in rabbit aortic neointima after selective de-endothelialization. Atherosclerosis 1996, 126: 95-104. PMID: 8879438, DOI: 10.1016/0021-9150(96)05898-4.Peer-Reviewed Original ResearchConceptsTIMP-1 geneRabbit formsApparent homologyExtracellular matrix metabolismExpression systemTIMP-1 expressionSoluble proteinNorthern blotSitu hybridization techniqueEscherichia coliProtein levelsMatrix metabolismGenesAortic neointimaNeointimal SMCsHybridization techniqueNucleic acidsWestern blotTIMP-1Tissue inhibitorExpressionBlotMetalloproteinase-1CDNAInhibitors