2004
Dose‐Dependent Neovascularization‐Promoting Effect of Adenovirus Vector CI‐1023 in a Rat Hindlimb Ischaemic Model
Wang H, Keiser J, Olszewski B, Brammer D, Gordon D. Dose‐Dependent Neovascularization‐Promoting Effect of Adenovirus Vector CI‐1023 in a Rat Hindlimb Ischaemic Model. Basic & Clinical Pharmacology & Toxicology 2004, 95: 76-80. PMID: 15379784, DOI: 10.1111/j.1742-7843.2004.950206.x.Peer-Reviewed Original ResearchConceptsHindlimb ischemic modelDose-dependent effectIschemic modelObvious dose-dependent effectDose rangeLocal tissue expressionVascular endothelial growth factor (VEGF) proteinReplication-deficient adenovirus vectorLaser Doppler scanningEfficacy of CIWide dose rangeGrowth factor proteinDoppler scanningImmuno-histochemistryTissue blood perfusionBlood perfusionTissue expressionAdenovirus vectorFactor proteinPrevious studiesAngiography
2000
Rat sponge implant model: a new system for evaluating angiogenic gene transfer.
Wang H, Gordon D, Olszewski B, Song Y, Kovesdi I, Keiser J. Rat sponge implant model: a new system for evaluating angiogenic gene transfer. International Journal Of Molecular Medicine 2000, 6: 645-53. PMID: 11078823, DOI: 10.3892/ijmm.6.6.645.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBeta-GalactosidaseBlood VesselsDNA, RecombinantDose-Response Relationship, DrugEndothelial Growth FactorsGene Transfer TechniquesGene Transfer, HorizontalHumansImmunohistochemistryLymphokinesModels, CardiovascularNeovascularization, PathologicProstheses and ImplantsRatsRecombinant Fusion ProteinsSurgical SpongesTime FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVon Willebrand FactorConceptsBeta-galactosidase reporter geneSuch genesReporter geneGenesGene transferTransfection efficiencyAngiogenic genesGene therapyProtein levelsProtein expressionVEGF121 geneReplication-deficient adenovirus vectorModel systemSponge implant modelAngiogenic responseProtein injectionAngiogenesisAdenovirus vectorVEGF protein expressionAngiogenic effectsProtein concentrationEnhanced angiogenesisAngiogenic efficacyGene transfection efficiencyHigh levelsHepatocyte Growth Factor Enhances MMP Activity in Human Endothelial Cells
Wang H, Keiser J. Hepatocyte Growth Factor Enhances MMP Activity in Human Endothelial Cells. Biochemical And Biophysical Research Communications 2000, 272: 900-905. PMID: 10860849, DOI: 10.1006/bbrc.2000.2852.Peer-Reviewed Original ResearchMeSH KeywordsCell LineCell MovementCollagenCoronary VesselsDermisDose-Response Relationship, DrugDrug CombinationsEndothelium, VascularEnzyme ActivationEnzyme InductionFlavonoidsHepatocyte Growth FactorHumansLamininMAP Kinase Signaling SystemMatrix Metalloproteinase 2Matrix Metalloproteinase InhibitorsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMitogen-Activated Protein KinasesMolecular WeightPhosphorylationProteoglycansProto-Oncogene Proteins c-metRNA, MessengerConceptsSF/HGFHepatocyte growth factorExtracellular matrixMAP kinase cascadeSignal transduction pathwaysScatter factorMT1-MMP synthesisMatrix metalloproteinasesGrowth factorHuman EC linesKinase cascadeTransduction pathwaysEC invasionHGF stimulationEndothelial cell productionHuman endothelial cellsMMP-2 activationArterial ECsMT1-MMPVessel stabilizationTube formationCurrent studyAngiogenic factorsEC linesMMP activity