Dose‐Dependent Neovascularization‐Promoting Effect of Adenovirus Vector CI‐1023 in a Rat Hindlimb Ischaemic Model
Wang H, Keiser J, Olszewski B, Brammer D, Gordon D. Dose‐Dependent Neovascularization‐Promoting Effect of Adenovirus Vector CI‐1023 in a Rat Hindlimb Ischaemic Model. Basic & Clinical Pharmacology & Toxicology 2004, 95: 76-80. PMID: 15379784, DOI: 10.1111/j.1742-7843.2004.950206.x.Peer-Reviewed Original ResearchConceptsHindlimb ischemic modelDose-dependent effectIschemic modelObvious dose-dependent effectDose rangeLocal tissue expressionVascular endothelial growth factor (VEGF) proteinReplication-deficient adenovirus vectorLaser Doppler scanningEfficacy of CIWide dose rangeGrowth factor proteinDoppler scanningImmuno-histochemistryTissue blood perfusionBlood perfusionTissue expressionAdenovirus vectorFactor proteinPrevious studiesAngiographyImpaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression
Wang H, Olszewski B, Rosebury W, Wang D, Robertson A, Keiser J. Impaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression. Biochemical And Biophysical Research Communications 2004, 315: 363-368. PMID: 14766216, DOI: 10.1016/j.bbrc.2004.01.059.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsCell DivisionDNA, ComplementaryDown-RegulationEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesHumansImmunoblottingImmunohistochemistryMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PathologicProliferating Cell Nuclear AntigenRatsRats, Inbred SHRRats, Inbred WKYRats, Sprague-DawleySignal TransductionSpectrophotometryTime FactorsUp-RegulationVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsSpontaneous hypertensive ratsKinase insert domain-containing receptorMT1-MMP expressionAge-matched normotensive Wistar-KyotoImpaired angiogenesisEndothelial proliferationHypertension animal modelsNormotensive Wistar-KyotoSponge implantation modelLevel of angiogenesisSprague-Dawley ratsVEGF gene transferHypertensive ratsWistar KyotoDomain-containing receptorMembrane type 1 matrix metalloproteinaseAnimal modelsImmunohistological analysisMatrix metalloproteinaseImpaired VEGFSponge implantationAngiogenesisVEGF addition