2008
Transgenic overexpression of a stable Plasminogen Activator Inhibitor-1 variant
Fahim A, Wang H, Feng J, Ginsburg D. Transgenic overexpression of a stable Plasminogen Activator Inhibitor-1 variant. Thrombosis Research 2008, 123: 785-792. PMID: 18774162, PMCID: PMC2670886, DOI: 10.1016/j.thromres.2008.07.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsLungMiceMice, TransgenicMutationPlasminogen Activator Inhibitor 1RNA, MessengerTransgenesConceptsPlasminogen activator inhibitor-1Plasma PAI-1 levelsPAI-1 levelsPAI-1 mRNAHigher plasma PAI-1 levelsTransgenic overexpressionEvidence of thrombosisActivator inhibitor-1PAI-1 variantsApparent thrombosisHistologic examinationSerine protease inhibitor gene familyPhysiologic effectsTransgenic miceChicken beta-actin promoterModerate expressionInhibitor-1High expressionBeta-actin promoterBiologic importanceThrombosisPhysiologic conditionsKidneyMiceLiver
2001
Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development.
Wang H, Liu C, Song Y, Gordon D, Alavi M, Moore S. Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development. International Journal Of Molecular Medicine 2001, 7: 105-12. PMID: 11115618, DOI: 10.3892/ijmm.7.1.105.Peer-Reviewed Original ResearchAnimalsBlotting, NorthernBlotting, WesternEndothelium, VascularGene Expression RegulationIn Situ HybridizationMaleMatrix Metalloproteinase 2Matrix Metalloproteinase 9MetalloendopeptidasesRabbitsRNA, MessengerTime FactorsTissue Inhibitor of Metalloproteinase-1Tissue Inhibitor of MetalloproteinasesTunica Intima
2000
Hepatocyte Growth Factor Enhances MMP Activity in Human Endothelial Cells
Wang H, Keiser J. Hepatocyte Growth Factor Enhances MMP Activity in Human Endothelial Cells. Biochemical And Biophysical Research Communications 2000, 272: 900-905. PMID: 10860849, DOI: 10.1006/bbrc.2000.2852.Peer-Reviewed Original ResearchMeSH KeywordsCell LineCell MovementCollagenCoronary VesselsDermisDose-Response Relationship, DrugDrug CombinationsEndothelium, VascularEnzyme ActivationEnzyme InductionFlavonoidsHepatocyte Growth FactorHumansLamininMAP Kinase Signaling SystemMatrix Metalloproteinase 2Matrix Metalloproteinase InhibitorsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMitogen-Activated Protein KinasesMolecular WeightPhosphorylationProteoglycansProto-Oncogene Proteins c-metRNA, MessengerConceptsSF/HGFHepatocyte growth factorExtracellular matrixMAP kinase cascadeSignal transduction pathwaysScatter factorMT1-MMP synthesisMatrix metalloproteinasesGrowth factorHuman EC linesKinase cascadeTransduction pathwaysEC invasionHGF stimulationEndothelial cell productionHuman endothelial cellsMMP-2 activationArterial ECsMT1-MMPVessel stabilizationTube formationCurrent studyAngiogenic factorsEC linesMMP activity
1998
Vascular Endothelial Growth Factor Upregulates the Expression of Matrix Metalloproteinases in Vascular Smooth Muscle Cells
Wang H, Keiser J. Vascular Endothelial Growth Factor Upregulates the Expression of Matrix Metalloproteinases in Vascular Smooth Muscle Cells. Circulation Research 1998, 83: 832-840. PMID: 9776730, DOI: 10.1161/01.res.83.8.832.Peer-Reviewed Original ResearchMeSH KeywordsAortaBiocompatible MaterialsCell DivisionCell MovementCells, CulturedCollagenCollagenasesDrug CombinationsEndothelial Growth FactorsEndothelium, VascularEnzyme ActivationEnzyme-Linked Immunosorbent AssayExtracellular MatrixGelsGene Expression Regulation, EnzymologicHumansLamininLymphokinesMatrix Metalloproteinase 1Matrix Metalloproteinase 9Muscle, Smooth, VascularNeovascularization, PhysiologicPhosphorylationProteoglycansProto-Oncogene ProteinsReceptor Protein-Tyrosine KinasesRNA, MessengerVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1Vascular Endothelial Growth FactorsConceptsHuman smooth muscle cellsKinase insert domain-containing receptorSmooth muscle cellsVascular smooth muscle cellsSMC migrationSignal transduction pathwaysVascular endothelial growth factorExtracellular matrix barriersMuscle cellsNorthern blot analysisMajor cell typesEffects of VEGFGrowth factorDomain-containing receptorTransduction pathwaysInsert domain-containing receptorFlt-1Endothelial cellsExpression of Membrane-Type Matrix Metalloproteinase in Rabbit Neointimal Tissue and Its Correlation with Matrix-Metalloproteinase-2 Activation
Wang H, Keiser J. Expression of Membrane-Type Matrix Metalloproteinase in Rabbit Neointimal Tissue and Its Correlation with Matrix-Metalloproteinase-2 Activation. Journal Of Vascular Research 1998, 35: 45-54. PMID: 9482695, DOI: 10.1159/000025564.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAortaBase SequenceBlotting, NorthernCells, CulturedDNA, ComplementaryEnzyme ActivationGelatinasesGene ExpressionHumansMaleMatrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMolecular Sequence DataMuscle, Smooth, VascularPolymerase Chain ReactionRabbitsRNA ProbesRNA, MessengerSequence HomologyConceptsBalloon catheter de-endothelializationMMP-2 activationArterial SMCsTemporal expression patternsMembrane-type matrix metalloproteinasesMembrane-type matrix metalloproteinaseNorthern blot analysisMatrix metalloproteinasesExtracellular matrix degradationRabbit cDNAMammalian cellsMT-MMP-1Matrix metalloproteinase-2 activationMT-MMP-1 expressionExpression patternsVascular neointimal formationSequence analysisMMP-2MMP cDNAPhenotypic alterationsCDNAPeak expressionRNA probesBlot analysisMatrix degradationCollagen biosynthesis by neointimal smooth muscle cells cultured from rabbit aortic explants 15 weeks after de‐endothelialization
WANG H, LI Z, MOORE S, ALAVI M. Collagen biosynthesis by neointimal smooth muscle cells cultured from rabbit aortic explants 15 weeks after de‐endothelialization. International Journal Of Experimental Pathology 1998, 79: 47-53. PMID: 9614349, PMCID: PMC3219429, DOI: 10.1046/j.1365-2613.1998.00048.x.Peer-Reviewed Original ResearchConceptsNeointimal smooth muscle cellsSmooth muscle cellsArterial neointimaCollagen accumulationMuscle cellsConfluent smooth muscle cellsBalloon catheter injuryExtracellular matrix accumulationCatheter injuryDe-endothelializationNormal aortaProcollagen IIIMatrix accumulationAortic neointimaMRNA expression
1997
Expression of syndecan-1 in rabbit neointima following de-endothelialization by a balloon catheter
Wang H, Moore S, Alavi M. Expression of syndecan-1 in rabbit neointima following de-endothelialization by a balloon catheter. Atherosclerosis 1997, 131: 141-147. PMID: 9199266, DOI: 10.1016/s0021-9150(97)06078-4.Peer-Reviewed Original ResearchConceptsSyndecan-1Low density lipoprotein retentionSyndecan-1 expressionArterial injuryBalloon catheterImmunohistochemical stainingEarly atherogenesisNeointimal cellsPositive cellsArterial neointimaRabbit aortaNormal aortaNeointimal tissueRabbit neointimaAortic neointimaNeointimaLipoprotein retentionCell membrane proteoglycansGrowth factorProtein levelsExtracellular matrix componentsNorthern blot analysisAortaBlot analysisInjury