2004
Impaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression
Wang H, Olszewski B, Rosebury W, Wang D, Robertson A, Keiser J. Impaired angiogenesis in SHR is associated with decreased KDR and MT1-MMP expression. Biochemical And Biophysical Research Communications 2004, 315: 363-368. PMID: 14766216, DOI: 10.1016/j.bbrc.2004.01.059.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsCell DivisionDNA, ComplementaryDown-RegulationEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesHumansImmunoblottingImmunohistochemistryMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PathologicProliferating Cell Nuclear AntigenRatsRats, Inbred SHRRats, Inbred WKYRats, Sprague-DawleySignal TransductionSpectrophotometryTime FactorsUp-RegulationVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsSpontaneous hypertensive ratsKinase insert domain-containing receptorMT1-MMP expressionAge-matched normotensive Wistar-KyotoImpaired angiogenesisEndothelial proliferationHypertension animal modelsNormotensive Wistar-KyotoSponge implantation modelLevel of angiogenesisSprague-Dawley ratsVEGF gene transferHypertensive ratsWistar KyotoDomain-containing receptorMembrane type 1 matrix metalloproteinaseAnimal modelsImmunohistological analysisMatrix metalloproteinaseImpaired VEGFSponge implantationAngiogenesisVEGF addition
2000
Rat sponge implant model: a new system for evaluating angiogenic gene transfer.
Wang H, Gordon D, Olszewski B, Song Y, Kovesdi I, Keiser J. Rat sponge implant model: a new system for evaluating angiogenic gene transfer. International Journal Of Molecular Medicine 2000, 6: 645-53. PMID: 11078823, DOI: 10.3892/ijmm.6.6.645.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBeta-GalactosidaseBlood VesselsDNA, RecombinantDose-Response Relationship, DrugEndothelial Growth FactorsGene Transfer TechniquesGene Transfer, HorizontalHumansImmunohistochemistryLymphokinesModels, CardiovascularNeovascularization, PathologicProstheses and ImplantsRatsRecombinant Fusion ProteinsSurgical SpongesTime FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVon Willebrand FactorConceptsBeta-galactosidase reporter geneSuch genesReporter geneGenesGene transferTransfection efficiencyAngiogenic genesGene therapyProtein levelsProtein expressionVEGF121 geneReplication-deficient adenovirus vectorModel systemSponge implant modelAngiogenic responseProtein injectionAngiogenesisAdenovirus vectorVEGF protein expressionAngiogenic effectsProtein concentrationEnhanced angiogenesisAngiogenic efficacyGene transfection efficiencyHigh levels