2010
Chapter 19 Thioredoxin and Redox Signaling in Vasculature—Studies Using Trx2 Endothelium-Specific Transgenic Mice
Min W, Xu L, Zhou H, Huang Q, Zhang H, He Y, Zhe X, Luo Y. Chapter 19 Thioredoxin and Redox Signaling in Vasculature—Studies Using Trx2 Endothelium-Specific Transgenic Mice. Methods In Enzymology 2010, 474: 315-324. PMID: 20609919, DOI: 10.1016/s0076-6879(10)74019-2.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesCellular reactive oxygen speciesMitochondrial antioxidant systemVivo functional assaysTRX2 geneRedox signalingTransgenic miceRedox genesThioredoxin 2Functional analysisHuman diseasesFunctional assaysAntioxidant systemOxygen speciesGenesCritical roleEndothelial cell culturesCell culturesPrimary lineTrx2TransgenesisThioredoxinMitochondriaSignalingSpecies
2007
Endothelial-Specific Expression of Mitochondrial Thioredoxin Improves Endothelial Cell Function and Reduces Atherosclerotic Lesions
Zhang H, Luo Y, Zhang W, He Y, Dai S, Zhang R, Huang Y, Bernatchez P, Giordano FJ, Shadel G, Sessa WC, Min W. Endothelial-Specific Expression of Mitochondrial Thioredoxin Improves Endothelial Cell Function and Reduces Atherosclerotic Lesions. American Journal Of Pathology 2007, 170: 1108-1120. PMID: 17322393, PMCID: PMC1864879, DOI: 10.2353/ajpath.2007.060960.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaApolipoproteins EAtherosclerosisCells, CulturedEndothelial CellsFlow CytometryImmunoblottingImmunohistochemistryMiceMice, TransgenicMicroscopy, ConfocalMitochondrial ProteinsNitric OxideReactive Oxygen SpeciesReverse Transcriptase Polymerase Chain ReactionThioredoxinsVasodilationConceptsTg miceAtherosclerotic lesionsOxidative stressNitric oxide levelsEC functionDeficient mouse modelEndothelial cell functionAtherosclerosis developmentEnhanced vasodilationVascular EC functionEndothelium functionApolipoprotein EControl littermatesMouse modelOxide levelsMice showCapacity of ECEndothelial-specific expressionEndothelial cellsCritical roleReactive oxygen speciesCell functionMiceTotal antioxidantsLesions