2020
Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein
Heo P, Pincet F. Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein. Communications Biology 2020, 3: 148. PMID: 32235856, PMCID: PMC7109109, DOI: 10.1038/s42003-020-0883-7.Peer-Reviewed Original ResearchMeSH KeywordsAlpha-SynucleinCell MembraneElectric CapacitanceFluorescence Recovery After PhotobleachingHydrophobic and Hydrophilic InteractionsLab-On-A-Chip DevicesMembrane FluidityMembrane LipidsMembrane PotentialsMembranes, ArtificialMicrofluidic Analytical TechniquesNeuronsProtein AggregatesProtein Aggregation, PathologicalProtein BindingProtein ConformationStructure-Activity RelationshipSynucleinopathiesConceptsPlasma membraneMembrane-bound proteinsAccumulation of aggregatesΑ-synucleinCytosolic leafletMembrane topologyMembrane hydrophobic coreCytosolic proteinsProteinExtracellular onesHydrophobic corePathological roleDiscrete sizesMembraneLeafletsMembrane capacitanceNeurological diseasesLipidsAccumulationMicrofluidic setup
2016
FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments
Pincet F, Adrien V, Yang R, Delacotte J, Rothman JE, Urbach W, Tareste D. FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments. PLOS ONE 2016, 11: e0158457. PMID: 27387979, PMCID: PMC4936743, DOI: 10.1371/journal.pone.0158457.Peer-Reviewed Original ResearchConceptsFluorescence correlation spectroscopySingle-particle trackingCorresponding recovery timeFRAP measurementsDynamics of lipidsDiffusion coefficientCorrelation spectroscopyBrownian motionParticle trackingConfocal microscopeAccurate valuesDiffusive speciesMembrane environmentMolecular diffusionMeasurementsMembrane platformsBehavior of lipidsFRAP experimentsMotion
2004
Hemifusion and fusion of giant vesicles induced by reduction of inter-membrane distance
Heuvingh J, Pincet F, Cribier S. Hemifusion and fusion of giant vesicles induced by reduction of inter-membrane distance. The European Physical Journal E 2004, 14: 269-276. PMID: 15338438, DOI: 10.1140/epje/i2003-10151-2.Peer-Reviewed Original ResearchConceptsInter-membrane distanceGiant unilamellar vesiclesInner leafletMembrane fusionOuter leafletMeans of micromanipulationCommon functionFluorescence microscopyProbe redistributionVesiclesInfluenza virus hemagglutininGiant vesiclesDNA basesModel systemHemifusionUnilamellar vesiclesActual fusionVirus hemagglutininMembraneContact zoneFusionHead groupsGood agreementProteinLeaflets
2002
Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid Layers
Tareste D, Pincet F, Perez E, Rickling S, Mioskowski C, Lebeau L. Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid Layers. Biophysical Journal 2002, 83: 3675-3681. PMID: 12496134, PMCID: PMC1302442, DOI: 10.1016/s0006-3495(02)75367-8.Peer-Reviewed Original ResearchConceptsHydrogen bondsIntramolecular hydrogen bondsHydrogen-bonding groupsSurface force apparatusSingle hydrogen bondHydrogen-bonding partsMonolayer of lipidsDifferent energetic contributionsForce apparatusHydrophobic interactionsInteraction energyBondsKcal/Lipid layerEnergetic contributionsTheoretical studyAdhesion energyProtein systemsBond partProtein foldingProtein stabilizationHeadgroupsWaterSurfaceComplex structure