2023
Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransporters
2018
Direct Measurement of Site-Specific Binding Energetics of Synaptotagmin-1 with Anionic Lipid Membranes
Gruget C, Coleman J, Bello O, Krishnakumar S, Pincet F, Rothman J, Donaldson S. Direct Measurement of Site-Specific Binding Energetics of Synaptotagmin-1 with Anionic Lipid Membranes. Biophysical Journal 2018, 114: 607a. DOI: 10.1016/j.bpj.2017.11.3318.Peer-Reviewed Original Research