2002
Alcohols enhance caerulein-induced zymogen activation in pancreatic acinar cells
Lu Z, Karne S, Kolodecik T, Gorelick FS. Alcohols enhance caerulein-induced zymogen activation in pancreatic acinar cells. AJP Gastrointestinal And Liver Physiology 2002, 282: g501-g507. PMID: 11842000, PMCID: PMC2830557, DOI: 10.1152/ajpgi.00388.2001.Peer-Reviewed Original Research
1998
Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury
Grady T, Mah’Moud M, Otani T, Rhee S, Lerch MM, Gorelick FS. Zymogen proteolysis within the pancreatic acinar cell is associated with cellular injury. American Journal Of Physiology 1998, 275: g1010-g1017. PMID: 9815031, DOI: 10.1152/ajpgi.1998.275.5.g1010.Peer-Reviewed Original ResearchConceptsPancreatic acinar cellsAcinar cellsCellular injuryForms of pancreatitisAcinar cell injuryTrypsinogen activation peptideSecretagogue treatmentPathological activationCell injuryInjuryIsolated aciniHyperstimulationBombesin treatmentPancreatic aciniTrypsinogen processingImmunofluorescence studiesBombesin stimulationAciniCA1TreatmentDigestive zymogensActivationCellsZymogen activationZymogen processing
1994
Calcium/calmodulin-dependent protein kinase-II activation in rat pituitary cells in the presence of thyrotropin-releasing hormone and dopamine
Cui ZJ, Gorelick FS, Dannies PS. Calcium/calmodulin-dependent protein kinase-II activation in rat pituitary cells in the presence of thyrotropin-releasing hormone and dopamine. Endocrinology 1994, 134: 2245-2250. PMID: 8156928, DOI: 10.1210/endo.134.5.8156928.Peer-Reviewed Original ResearchConceptsPulses of TRHPRL releaseCaM kinase IIPituitary cellsAnterior pituitary cellsCaM kinase II activityThyrotropin-releasing hormoneRat pituitary cellsCalmodulin-dependent protein kinase II activationCalcium/calmodulin-dependent protein kinase II (CaMKII) activationKinase IICalcium/calmodulin-dependent protein kinase IIPretreatment of cellsCalmodulin-dependent protein kinase IIBasal valuesKinase II activityLack of responsivenessKinase activityIntracellular Ca2TRHProtein kinase IIRat lactotrophsDopamineTRH pulsesLactotrophs
1991
Agonist-regulated phosphorylation of the pancreatic cholecystokinin receptor
Klueppelberg UG, Gates LK, Gorelick FS, Miller LJ. Agonist-regulated phosphorylation of the pancreatic cholecystokinin receptor. Journal Of Biological Chemistry 1991, 266: 2403-2408. PMID: 1989991, DOI: 10.1016/s0021-9258(18)52258-4.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCarbacholCell FractionationChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEnzyme ActivationKineticsMalePancreasPhosphorylationProtein Kinase CRatsRats, Inbred StrainsReceptors, CholecystokininSincalideSolubilityTetradecanoylphorbol AcetateConceptsC activationSodium dodecyl sulfate-polyacrylamide gelsDodecyl sulfate-polyacrylamide gelsProtein kinase C activationPlasma membrane glycoproteinsPhosphorylated MrKinase C activationSulfate-polyacrylamide gelsSerine residuesRegulated mannerAffinity-labeled proteinsMembrane glycoproteinsPhosphorylationReceptor proteinProteinNative formATP poolAffinity resinLectin affinity chromatographyConcentration-dependent mannerDirect activationPancreatic cholecystokinin receptorSubsequent solubilizationReceptorsActivation
1990
Regulated phosphorylation of secretory granule membrane proteins of the rat parotid gland
Marino CR, Castle JD, Gorelick FS. Regulated phosphorylation of secretory granule membrane proteins of the rat parotid gland. American Journal Of Physiology 1990, 259: g70-g77. PMID: 1695488, DOI: 10.1152/ajpgi.1990.259.1.g70.Peer-Reviewed Original ResearchConceptsSecretory granule membrane proteinsIntegral membrane proteinsMembrane proteinsGranule membrane proteinProtein phosphorylation eventsGranule membrane fractionExocrine secretory granulesPhosphorylation eventsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisRat parotid glandRegulated phosphorylationSulfate-polyacrylamide gel electrophoresisSitu phosphorylationIntact cellsMembrane fractionTime-dependent mannerPhosphorylationProteinSecretory granule fractionParotid lobulesGel electrophoresisProtein antiserumSecretory granulesPhosphoprotein
1989
Translocation of synapsin I in response to depolarization of isolated nerve terminals.
Sihra TS, Wang JK, Gorelick FS, Greengard P. Translocation of synapsin I in response to depolarization of isolated nerve terminals. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8108-8112. PMID: 2510160, PMCID: PMC298224, DOI: 10.1073/pnas.86.20.8108.Peer-Reviewed Original ResearchConceptsSynapsin INerve terminalsRelease of neurotransmittersDepolarization of synaptosomesSmall synaptic vesiclesNeurotransmitter releaseSynaptic vesiclesDependent mannerIncubation mediumNerve terminal phosphoproteinDepolarizationPresent studyPhosphorylation stateCytosolic fractionPresence of Ca2TranslocationCa2Particulate fraction
1988
Autophosphorylation and activation of Ca2+/calmodulin-dependent protein kinase II in intact nerve terminals.
Gorelick FS, Wang JK, Lai Y, Nairn AC, Greengard P. Autophosphorylation and activation of Ca2+/calmodulin-dependent protein kinase II in intact nerve terminals. Journal Of Biological Chemistry 1988, 263: 17209-17212. PMID: 2846557, DOI: 10.1016/s0021-9258(19)77816-8.Peer-Reviewed Original ResearchConceptsDependent protein kinase IIKinase IIAlpha subunitProtein kinase IIKinase II activityTwo-dimensional phosphopeptide mapsII activityState of phosphorylationAutophosphorylation mechanismThreonine residuesPhosphothreonine contentPhosphopeptide mapsTransient phosphorylationIndependent speciesPhosphoserine contentIntact nerve terminalsBeta subunitEnhanced phosphorylationSubunitsPhosphorylationAutophosphorylationIntact synaptosomesBasal incubation conditionsPhosphopeptidesDepolarization of synaptosomes
1987
Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas
Cohn J, Kinder B, Jamieson J, Delahunt N, Gorelick F. Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas. Biochimica Et Biophysica Acta 1987, 928: 320-331. PMID: 3105599, DOI: 10.1016/0167-4889(87)90192-3.Peer-Reviewed Original ResearchConceptsCalmodulin protein kinaseCalcium/calmodulin-dependent protein kinaseCalmodulin-dependent protein kinaseProtein kinaseMultifunctional calcium/calmodulin-dependent protein kinaseTwo-dimensional gel electrophoresisRibosomal proteinsThreonine residuesLarge subunitCellular functionsRibosomal substratesSubstrate specificityKinaseProteolytic degradationSubunitsGel electrophoresisHydrophobic chromatographyAffinity chromatographyPolyacrylamide gelsCalmodulinPurification procedureGel filtrationEnzyme preparationAutophosphorylationTissue