1993
Phosphorylation of elongation factor 2 in normal and malignant rat glial cells.
Bagaglio DM, Cheng EH, Gorelick FS, Mitsui K, Nairn AC, Hait WN. Phosphorylation of elongation factor 2 in normal and malignant rat glial cells. Cancer Research 1993, 53: 2260-4. PMID: 8485712.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium-Calmodulin-Dependent Protein KinasesCalmodulinCell DivisionCells, CulturedElongation Factor 2 KinaseGliomaMaleNeurogliaPeptide Elongation Factor 2Peptide Elongation FactorsPhosphorylationPrecipitin TestsProtein KinasesRatsRats, Sprague-DawleyTrifluoperazineTumor Cells, CulturedConceptsRat brain white matterNormal glial tissueGlial tissueGlioma cellsC6 cellsC6 rat glioma cellsCaM kinase IIIRat glial cellsFactor 2Rat glioma cellsBrain white matterNormal gliaElongation factor 2Glial cellsRat brainWhite matterTumor tissueBasal levelsIII activityCellular proliferationTissueDependent proteinsCellsEndogenous substratesHomogenatesDistribution of calcium/calmodulin-dependent protein kinase II in rat ileal enterocytes
Matovcik LM, Haimowitz B, Goldenring JR, Czernik AJ, Gorelick FS. Distribution of calcium/calmodulin-dependent protein kinase II in rat ileal enterocytes. American Journal Of Physiology 1993, 264: c1029-c1036. PMID: 8386447, DOI: 10.1152/ajpcell.1993.264.4.c1029.Peer-Reviewed Original ResearchConceptsProtein kinase IIDependent protein kinase IIKinase IICalmodulin-dependent protein kinase IICalcium/calmodulin-dependent protein kinase IIDependent kinase activityRelative molecular massSpecific peptide inhibitorWide tissue distributionTerminal web regionIntestinal epithelial cellsEpithelial cytoskeletonKinase activityMyosin IISoluble subcellular fractionMolecular massRat ilealRat ileumMultiple substratesEnterocyte cytoskeletonImmunoreactive proteinMajor effectorPeptide inhibitorEpithelial cellsTissue distributionCharacterization of cAMP‐dependent protein kinase activation by CCK in rat pancreas
Marino C, Leach S, Schaefer J, Miller L, Gorelick F. Characterization of cAMP‐dependent protein kinase activation by CCK in rat pancreas. FEBS Letters 1993, 316: 48-52. PMID: 7678554, PMCID: PMC2830555, DOI: 10.1016/0014-5793(93)81734-h.Peer-Reviewed Original ResearchConceptsCAMP-dependent protein kinase activityProtein kinase activityKinase activityCAMP second messenger cascadeCAMP-dependent protein kinase activationProtein kinase activationSecond messenger cascadesCellular cAMP levelsKinase activationCAMP cascadeTreatment of aciniMessenger cascadesCellular cAMPCAMP levelsEnzyme secretionCascadeNew sensitive assayRat pancreatic aciniSensitive assayPancreatic aciniActivityHigh concentrationsAciniCellsActivation
1992
Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I
Benfenati F, Valtorta F, Rubenstein J, Gorelick F, Greengard P, Czernik A. Synaptic vesicle-associated Ca2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I. Nature 1992, 359: 417-420. PMID: 1328883, DOI: 10.1038/359417a0.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrainCalcium-Calmodulin-Dependent Protein KinasesChromatography, High Pressure LiquidElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelGene Expression Regulation, EnzymologicMolecular Sequence DataPhosphorylationProtein KinasesRatsReceptors, NeurotransmitterSubstrate SpecificitySynapsinsSynaptic VesiclesConceptsDependent protein kinase IIProtein kinase IIC-terminal regionKinase IISynapsin ISynaptic vesicle-associated phosphoproteinsAmino-terminal regionCarboxy-terminal regionKinase functionRegulatory domainProtein componentsMembrane phospholipidsProteinPhosphoproteinVesiclesEnzymeRegionBindingPhospholipidsDomainSynaptic
1990
Rabbit ileal villus cell brush border Na+/H+ exchange is regulated by Ca2+/calmodulin-dependent protein kinase II, a brush border membrane protein.
Cohen ME, Reinlib L, Watson AJ, Gorelick F, Rys-Sikora K, Tse M, Rood RP, Czernik AJ, Sharp GW, Donowitz M. Rabbit ileal villus cell brush border Na+/H+ exchange is regulated by Ca2+/calmodulin-dependent protein kinase II, a brush border membrane protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8990-8994. PMID: 2174171, PMCID: PMC55086, DOI: 10.1073/pnas.87.22.8990.Peer-Reviewed Original ResearchConceptsDependent protein kinase IIProtein kinase IIKinase IIMembrane proteinsSynapsin IDependent protein kinase activityDependent protein kinaseBrush border membranePhosphorylation of sitesProtein kinase activityInhibitor peptideCaM kinase IIBrush border membrane proteinsBorder membraneSpecific inhibitor peptidePhosphopeptide mappingBrush borderProtein kinaseMicrovillus membrane proteinsKinase activityIleal brush-border membraneVillus cell brush border membraneApical membraneKinaseVesicle preparations
1989
Presence of calcium/calmodulin‐dependent protein Kinase II in Nerve terminals of rat brain
Walaas S, Gorelick F, Greengard P. Presence of calcium/calmodulin‐dependent protein Kinase II in Nerve terminals of rat brain. Synapse 1989, 3: 356-362. PMID: 2545012, DOI: 10.1002/syn.890030409.Peer-Reviewed Original ResearchConceptsCalcium/calmodulin-dependent protein kinase IICalmodulin-dependent protein kinase IISubstantia nigraProtein kinase IINerve terminalsRat brainSynapsin INerve terminal markersLesion-induced degenerationLesion-induced changesCalcium/calmodulin-dependent protein kinase type IIPrevious immunocytochemical studiesKinase IIStriatonigral tractCorticostriatal tractPresynaptic terminalsNeuronal populationsCalmodulin-dependent protein kinase type IIMammalian brainCell bodiesImmunocytochemical studyTerminal markersBrainNeostriatumLesions
1988
Cell-specific localization of the alpha-subunit of calcium/calmodulin-dependent protein kinase II in Purkinje cells in rodent cerebellum.
Walaas SI, Lai Y, Gorelick FS, DeCamilli P, Moretti M, Greengard P. Cell-specific localization of the alpha-subunit of calcium/calmodulin-dependent protein kinase II in Purkinje cells in rodent cerebellum. Brain Research 1988, 464: 233-42. PMID: 2850084, DOI: 10.1016/0169-328x(88)90029-0.Peer-Reviewed Original ResearchConceptsCalmodulin-dependent protein kinase type IICalcium/calmodulin-dependent protein kinase type IIProtein kinase type IIKinase type IICalmodulin-dependent protein kinase IICalcium/calmodulin-dependent protein kinase IIDistinct isozymic formsProtein kinase IINormal rat cerebellumPurkinje cellsBeta/betaNon-Purkinje cellsKinase IIAlpha subunitCell-specific localizationRestricted localizationRodent cerebellumIsozymic formsDifferent cellsImmunocytochemical analysisMutant miceSubunitsType IICellsCerebellumAutophosphorylation and activation of Ca2+/calmodulin-dependent protein kinase II in intact nerve terminals.
Gorelick FS, Wang JK, Lai Y, Nairn AC, Greengard P. Autophosphorylation and activation of Ca2+/calmodulin-dependent protein kinase II in intact nerve terminals. Journal Of Biological Chemistry 1988, 263: 17209-17212. PMID: 2846557, DOI: 10.1016/s0021-9258(19)77816-8.Peer-Reviewed Original ResearchConceptsDependent protein kinase IIKinase IIAlpha subunitProtein kinase IIKinase II activityTwo-dimensional phosphopeptide mapsII activityState of phosphorylationAutophosphorylation mechanismThreonine residuesPhosphothreonine contentPhosphopeptide mapsTransient phosphorylationIndependent speciesPhosphoserine contentIntact nerve terminalsBeta subunitEnhanced phosphorylationSubunitsPhosphorylationAutophosphorylationIntact synaptosomesBasal incubation conditionsPhosphopeptidesDepolarization of synaptosomesCa2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity.
Thiel G, Czernik AJ, Gorelick F, Nairn AC, Greengard P. Ca2+/calmodulin-dependent protein kinase II: identification of threonine-286 as the autophosphorylation site in the alpha subunit associated with the generation of Ca2+-independent activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 6337-6341. PMID: 2842767, PMCID: PMC281965, DOI: 10.1073/pnas.85.17.6337.Peer-Reviewed Original ResearchConceptsBeta/beta' subunitsAlpha subunitThr-286Beta subunitDependent protein kinase IIProtein kinase IIAutophosphorylation sitesThreonine residuesMajor phosphopeptideNaDodSO4/PAGEPhosphorylated residuesCyanogen bromide peptidesConsensus sequenceKinase IIIndependent activityThermolytic phosphopeptidesPrimary structureGas-phase Edman degradationGeneration of Ca2Edman degradationAutophosphorylationSubunitsThreonine-286Amino acidsAsp-XaaDevelopment of Secretagogue Responsiveness in the Pancreas
Jamieson JD, Gorelick FS, Chang A. Development of Secretagogue Responsiveness in the Pancreas. Scandinavian Journal Of Gastroenterology 1988, 23: 98-103. PMID: 2852401, DOI: 10.3109/00365528809095920.Peer-Reviewed Original ResearchConceptsSecretory pathwaySecretory proteinsPancreatic acinar cellsEpithelial cell polarityDependent protein kinase IIRegulated secretory pathwayConstitutive secretory pathwayAcinar cellsProtein kinase IICell polarityMembrane proteinsPhosphorylated substratesRegulated secretionBasolateral domainCell biologyPlasmalemmal domainsPlasma membraneKinase IISecond messengerSecretagogue responsivenessFunctional specializationProteinEpithelial cellsPathwayZymogen granules
1987
Calcium-calmodulin-stimulated protein kinase in developing pancreas
Gorelick FS, Chang A, Jamieson JD. Calcium-calmodulin-stimulated protein kinase in developing pancreas. American Journal Of Physiology 1987, 253: g469-g476. PMID: 3116856, DOI: 10.1152/ajpgi.1987.253.4.g469.Peer-Reviewed Original ResearchConceptsCalmodulin-dependent protein kinaseProtein kinase activityCDPK activityProtein kinaseKinase activityEndogenous proteinsType II calmodulin-dependent protein kinaseType II Ca2Adult pancreasCaM-binding proteinsRelative molecular weightEmbryonic pancreasPancreatic developmentPancreatic acinar cellsGel filtration chromatographySecretagogue responsivenessCalcium-calmodulinExogenous substratesKinaseSynapsin IPancreatic maturationProteinPancreatic supernatantPhosphorylationSecretory processCa2+/calmodulin-dependent protein kinase II: identification of autophosphorylation sites responsible for generation of Ca2+/calmodulin-independence.
Lai Y, Nairn AC, Gorelick F, Greengard P. Ca2+/calmodulin-dependent protein kinase II: identification of autophosphorylation sites responsible for generation of Ca2+/calmodulin-independence. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 5710-5714. PMID: 3475699, PMCID: PMC298932, DOI: 10.1073/pnas.84.16.5710.Peer-Reviewed Original ResearchPurification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas
Cohn J, Kinder B, Jamieson J, Delahunt N, Gorelick F. Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas. Biochimica Et Biophysica Acta 1987, 928: 320-331. PMID: 3105599, DOI: 10.1016/0167-4889(87)90192-3.Peer-Reviewed Original ResearchConceptsCalmodulin protein kinaseCalcium/calmodulin-dependent protein kinaseCalmodulin-dependent protein kinaseProtein kinaseMultifunctional calcium/calmodulin-dependent protein kinaseTwo-dimensional gel electrophoresisRibosomal proteinsThreonine residuesLarge subunitCellular functionsRibosomal substratesSubstrate specificityKinaseProteolytic degradationSubunitsGel electrophoresisHydrophobic chromatographyAffinity chromatographyPolyacrylamide gelsCalmodulinPurification procedureGel filtrationEnzyme preparationAutophosphorylationTissueSecond messenger systems and adaptation.
Gorelick FS. Second messenger systems and adaptation. Gut 1987, 28: 79. PMID: 2826313, PMCID: PMC1434555, DOI: 10.1136/gut.28.suppl.79.Peer-Reviewed Original ResearchCalcium-Calmodulin-Dependent Protein Kinase in Hyperplastic Human Parathyroid Glands
KINDER BK, DELAHUNT NG, JAMIESON JD, GORELICK FS. Calcium-Calmodulin-Dependent Protein Kinase in Hyperplastic Human Parathyroid Glands. Endocrinology 1987, 120: 170-177. PMID: 3096699, DOI: 10.1210/endo-120-1-170.Peer-Reviewed Original Research
1983
Calmodulin-stimulated protein kinase activity from rat pancreas.
Gorelick FS, Cohn JA, Freedman SD, Delahunt NG, Gershoni JM, Jamieson JD. Calmodulin-stimulated protein kinase activity from rat pancreas. Journal Of Cell Biology 1983, 97: 1294-1298. PMID: 6619194, PMCID: PMC2112629, DOI: 10.1083/jcb.97.4.1294.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium-Binding ProteinsCalmodulinMolecular WeightPancreasPhosphorylationProtein KinasesRatsRibosomal Proteins