2007
Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks
Neelakanta G, Li X, Pal U, Liu X, Beck DS, DePonte K, Fish D, Kantor FS, Fikrig E. Outer Surface Protein B Is Critical for Borrelia burgdorferi Adherence and Survival within Ixodes Ticks. PLOS Pathogens 2007, 3: e33. PMID: 17352535, PMCID: PMC1817655, DOI: 10.1371/journal.ppat.0030033.Peer-Reviewed Original Research
2006
Borrelia burgdorferi Lacking BBK32, a Fibronectin-Binding Protein, Retains Full Pathogenicity
Li X, Liu X, Beck DS, Kantor FS, Fikrig E. Borrelia burgdorferi Lacking BBK32, a Fibronectin-Binding Protein, Retains Full Pathogenicity. Infection And Immunity 2006, 74: 3305-3313. PMID: 16714558, PMCID: PMC1479267, DOI: 10.1128/iai.02035-05.Peer-Reviewed Original ResearchMeSH KeywordsAdhesins, BacterialAnimalsBacterial AdhesionBacterial ProteinsBorrelia burgdorferiLyme DiseaseMiceMice, Inbred C3HRabbitsTicksConceptsB. burgdorferiFlat ticksB. burgdorferi proteinsTick biteSpirochete acquisitionLyme disease spirocheteAdverse effectsBorrelia burgdorferiFibronectin-binding proteinBurgdorferiFibronectin binding proteinMiceSpirochete life cycleLyme pathogenesisB. burgdorferi B31Mammalian hostsSyringe inoculationExpression profilesBBK32Surface lipoproteinsFibronectin bindingPathogenesisExpressionInfectionTemporal expression profiles
2004
Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection
Carlyon JA, Latif D, Pypaert M, Lacy P, Fikrig E. Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection. Infection And Immunity 2004, 72: 4772-4783. PMID: 15271939, PMCID: PMC470610, DOI: 10.1128/iai.72.8.4772-4783.2004.Peer-Reviewed Original ResearchConceptsPhorbol myristate acetateUnique tropismA. phagocytophilumNADPH oxidase assemblyOxidative killingNADPH oxidaseAnaplasma phagocytophilumSerum-opsonized zymosanA. phagocytophilum infectionHost evasion mechanismNeutrophil infectionOxygen species productionEvasion mechanismsEtiologic agentNADPH oxidase complexHuman anaplasmosisNeutrophilsPhagocytophilum infectionSecondary activationMyristate acetateDependent decreaseInitial stimulationNeutrophil plasma membranesSpecies productionPhagocytophilumOspB Antibody Prevents Borrelia burgdorferi Colonization of Ixodes scapularis
Fikrig E, Pal U, Chen M, Anderson JF, Flavell RA. OspB Antibody Prevents Borrelia burgdorferi Colonization of Ixodes scapularis. Infection And Immunity 2004, 72: 1755-1759. PMID: 14977984, PMCID: PMC356050, DOI: 10.1128/iai.72.3.1755-1759.2004.Peer-Reviewed Original Research
2003
Murine neutrophils require α1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum
Carlyon JA, Akkoyunlu M, Xia L, Yago T, Wang T, Cummings RD, McEver RP, Fikrig E. Murine neutrophils require α1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. Blood 2003, 102: 3387-3395. PMID: 12869507, DOI: 10.1182/blood-2003-02-0621.Peer-Reviewed Original ResearchConceptsWild-type miceP-selectin glycoprotein ligand-1Murine neutrophilsCommon tick-borne diseaseAnaplasma phagocytophilumFuc-TIVHuman granulocytic ehrlichiosisPSGL-1 expressionTick-borne diseaseNeutrophil expressionInfection burdenMurine infectionNeutrophilsGranulocytic ehrlichiosisPhagocytophilum infectionMiceHuman neutrophilsSimilar molecular featuresInfectionProductive infectionSialidase treatmentLigand 1PhagocytophilumMolecular featuresFuc-TVIIStructurally Distinct Requirements for Binding of P-selectin Glycoprotein Ligand-1 and Sialyl Lewis x to Anaplasma phagocytophilum and P-selectin*
Yago T, Leppänen A, Carlyon JA, Akkoyunlu M, Karmakar S, Fikrig E, Cummings RD, McEver RP. Structurally Distinct Requirements for Binding of P-selectin Glycoprotein Ligand-1 and Sialyl Lewis x to Anaplasma phagocytophilum and P-selectin*. Journal Of Biological Chemistry 2003, 278: 37987-37997. PMID: 12847092, DOI: 10.1074/jbc.m305778200.Peer-Reviewed Original Research
2000
Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
Pal U, de Silva A, Montgomery R, Fish D, Anguita J, Anderson J, Lobet Y, Fikrig E. Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A. Journal Of Clinical Investigation 2000, 106: 561-569. PMID: 10953031, PMCID: PMC380253, DOI: 10.1172/jci9427.Peer-Reviewed Original Research
1993
A monoclonal antibody to OspA inhibits association of Borrelia burgdorferi with human endothelial cells
Comstock LE, Fikrig E, Shoberg RJ, Flavell RA, Thomas DD. A monoclonal antibody to OspA inhibits association of Borrelia burgdorferi with human endothelial cells. Infection And Immunity 1993, 61: 423-431. PMID: 7678585, PMCID: PMC302746, DOI: 10.1128/iai.61.2.423-431.1993.Peer-Reviewed Original ResearchConceptsEndothelial cellsB. burgdorferiMonoclonal antibodiesInhibitory effectHUVE cellsBorrelia burgdorferiCultured human umbilical vein endothelial cellsNorth American strainsLevel of adherenceHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsInhibition of adherenceImmunoglobulin G fractionVein endothelial cellsHuman endothelial cellsSurface proteinsAmerican strainsOspA epitopeOuter surface proteinsInhibitory actionPenetration assayMAbsG fractionAntibodiesBurgdorferi