2007
Antibodies Targeting Linear Determinants of the Envelope Protein Protect Mice against West Nile Virus
Ledizet M, Kar K, Foellmer HG, Bonafé N, Anthony KG, Gould LH, Bushmich SL, Fikrig E, Koski RA. Antibodies Targeting Linear Determinants of the Envelope Protein Protect Mice against West Nile Virus. The Journal Of Infectious Diseases 2007, 196: 1741-1748. PMID: 18190253, DOI: 10.1086/523654.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, ViralAntibody AffinityChlorocebus aethiopsDrosophilaEpitopesFemaleFluorescent Antibody TechniqueHorsesImmunization, PassiveImmunoglobulinsMiceMice, Inbred C3HMolecular Sequence DataPeptide FragmentsVero CellsViral Envelope ProteinsWest Nile FeverWest Nile virusConceptsE proteinMultiple flavivirus infectionsVirus-neutralizing antibodiesE protein epitopesFlavivirus envelope proteinProtect miceWest Nile virusFlavivirus infectionLethal challengeProtective antibodiesWest Nile virus E proteinProtective epitopesVirus E proteinRelated flavivirusesImmunoglobulinAntibodiesNile virusLinear determinantsEpitopesEnvelope proteinProtein epitopesMiceHost cell membraneWNVPeptides
1997
Protective antibodies develop, and murine Lyme arthritis regresses, in the absence of MHC class II and CD4+ T cells.
Fikrig E, Barthold SW, Chen M, Chang CH, Flavell RA. Protective antibodies develop, and murine Lyme arthritis regresses, in the absence of MHC class II and CD4+ T cells. The Journal Of Immunology 1997, 159: 5682-6. PMID: 9548512, DOI: 10.4049/jimmunol.159.11.5682.Peer-Reviewed Original ResearchConceptsMHC class IIT cellsClass IINaive C3H/HeN miceC3H/HeN miceC57/BL6 miceCIITA-deficient miceRegression of arthritisResolution of arthritisResolution of carditisDevelopment of arthritisMurine Lyme borreliosisMHC class II transactivatorClass II moleculesClass II transactivatorIgG2b AbsProtective antibodiesBL6 miceHeN miceDeficient miceProtective AbsSCID micePersistent infectionArthritisCD4 repertoire
1996
Protective antibodies in murine Lyme disease arise independently of CD40 ligand.
Fikrig E, Barthold SW, Chen M, Grewal IS, Craft J, Flavell RA. Protective antibodies in murine Lyme disease arise independently of CD40 ligand. The Journal Of Immunology 1996, 157: 1-3. PMID: 8683101, DOI: 10.4049/jimmunol.157.1.1.Peer-Reviewed Original ResearchConceptsCD40L-deficient miceCD40 ligand-deficient miceMurine Lyme diseaseRegression of arthritisTransfer of serumDependent immune responsesIgG2b AbsAcute arthritisControl miceProtective antibodiesIg class switchingCD40 ligandImmune responseImmunodeficient miceLyme borreliosisLyme diseaseMiceBorrelia burgdorferiB. burgdorferiClass switchingArthritisBurgdorferiAbInfectionDisease
1995
An ospA frame shift, identified from DNA in Lyme arthritis synovial fluid, results in an outer surface protein A that does not bind protective antibodies.
Fikrig E, Liu B, Fu LL, Das S, Smallwood JI, Flavell RA, Persing DH, Schoen RT, Barthold SW, Malawista SE. An ospA frame shift, identified from DNA in Lyme arthritis synovial fluid, results in an outer surface protein A that does not bind protective antibodies. The Journal Of Immunology 1995, 155: 5700-4. PMID: 7499856, DOI: 10.4049/jimmunol.155.12.5700.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupFemaleFrameshift MutationHumansLipoproteinsLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataProtein BindingSynovial FluidConceptsSurface protein AOuter surface protein ASynovial fluidChronic Lyme arthritisSynovial fluid samplesSeparate time pointsImmune effectivenessLyme arthritisPassive immunizationProtective antibodiesHuman infectionsHuman AbsProtein ATime pointsNatural infectionInfectionBorrelia burgdorferiMiceOnly factorHuman hostFluid samplesOspAInfected hostHuman materialMicrobial persistenceSelection of variant Borrelia burgdorferi isolates from mice immunized with outer surface protein A or B
Fikrig E, Tao H, Barthold SW, Flavell RA. Selection of variant Borrelia burgdorferi isolates from mice immunized with outer surface protein A or B. Infection And Immunity 1995, 63: 1658-1662. PMID: 7729870, PMCID: PMC173206, DOI: 10.1128/iai.63.5.1658-1662.1995.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupCloning, MolecularFemaleGenes, BacterialGenetic VariationImmunoblottingLipoproteinsLyme DiseaseMiceMice, Inbred C3HRecombinant ProteinsSelection, GeneticSequence Analysis, DNAVaccinationConceptsSurface protein AOuter surface protein AProtective immunityProtective antibodiesIncomplete efficacyMiceMonoclonal antibodiesProtein ABorrelia burgdorferiB. burgdorferiAntigenic diversityOspBOspAVivo selective pressureImmunityAntibodiesB. burgdorferi isolatesBurgdorferiChallenge inoculationPoint mutationsMolecular basisVaccineInfection
1993
Evasion of protective immunity by Borrelia burgdorferi by truncation of outer surface protein B.
Fikrig E, Tao H, Kantor FS, Barthold SW, Flavell RA. Evasion of protective immunity by Borrelia burgdorferi by truncation of outer surface protein B. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 4092-4096. PMID: 7683420, PMCID: PMC46452, DOI: 10.1073/pnas.90.9.4092.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesAntibodies, MonoclonalAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBase SequenceBorrelia burgdorferi GroupCloning, MolecularCodonEpitopesEscherichia coliFemaleFluorescent Antibody TechniqueGenes, BacterialHumansLyme DiseaseMiceMice, Inbred C3HPolymerase Chain ReactionRecombinant Fusion ProteinsRecombinant ProteinsRestriction MappingTicksVaccines, SyntheticViral VaccinesConceptsProtective immune responseVaccination immunityProtective immunityProtective antibodiesAntibody responseImmune destructionImmune responseHost defenseOuter surface proteinsLyme diseaseOuter surface protein BBorrelia burgdorferiCausative agentOspBMicePolyclonal antibodiesImmunityAntibodiesPresent studyStrain B31Surface proteinsProtein BPrevious studiesVaccinationOspB.