Edward Manning, MD, PhD
Assistant Professor of Medicine (Pulmonary)Cards
About
Research
Publications
2026
Postnatal pulmonary artery development from transcript to tissue
Schwarz E, Ramachandra A, Yeung N, Manning E, Weiss D, Humphrey J. Postnatal pulmonary artery development from transcript to tissue. Journal Of The Royal Society Interface 2026, 23: 20250962. PMID: 42303255, DOI: 10.1098/rsif.2025.0962.Peer-Reviewed Original ResearchConceptsPulmonary artery developmentProximal pulmonary arteriesPulmonary arterySurgical interventionArtery developmentPostnatal developmentPatent ductus arteriosusWild-type miceFontan procedurePulmonary atresiaPulmonary hypertensionDuctus arteriosusSignificant morbidityBiomechanical propertiesGene expressionCongenital conditionPostnatal changesArteryVascular structuresArterial haemodynamicsPathological conditionsHaemodynamicsFontanArteriosusAtresiaGrowth arrest of thoracic aortic aneurysms in aging Marfan mice
Means C, Mays G, Yeung N, Cavinato C, Manning E, Lam T, Humphrey J, Weiss D. Growth arrest of thoracic aortic aneurysms in aging Marfan mice. AJP Heart And Circulatory Physiology 2026, 331: h115-h129. PMID: 42222959, DOI: 10.1152/ajpheart.00007.2026.Peer-Reviewed Original ResearchConceptsMouse model of Marfan syndromeModel of Marfan syndromeThoracic aortic aneurysmMarfan syndromeMouse modelGrowth arrestAortic aneurysmLimit disease progressionGenetic conditionsMarfan miceProgressive diseaseAneurysm expansionThoracic aortopathyDisease progressionHistological characteristicsExpressed genesTranscriptional profilesProtein abundanceAneurysmLesion growthAssociated proteinSyndromeGene expressionProteomic signaturePathological mechanismsB77-08 Modified RNA Extraction Method to Eliminate Agarose Carryover Interferences in Precision Cut Lung Slices
Marti Munoz J, Rangel R, Anderson S, Deiuliis G, Manning E, Ahangari F, Pandit A, Kaminski N. B77-08 Modified RNA Extraction Method to Eliminate Agarose Carryover Interferences in Precision Cut Lung Slices. American Journal Of Respiratory And Critical Care Medicine 2026, 212: aamag162.5390. DOI: 10.1093/ajrccm/aamag162.5390.Peer-Reviewed Original ResearchPlant KitRNA extraction methodsIntact rRNARNA extractionRNA quantityPrecision cut lung slicesExtraction methodYield of RNATranscriptional integrityPlantsYieldGene expressionRNA yieldRNA integrityRNARRNALung slicesAgaroseCulture conditionsExtractionStudy biologyEthidium homodimer-1Human lungRNA quantificationInstitutional review boardA74-12 An Ex Vivo Model of Induced Lung Aging Reveals Age-dependent Predisposition to Oxidative Stress and Cellular Senescence
De Man R, Rangel R, Marti Munoz J, Khoury J, Anderson S, Lin L, Adams T, Ahangari F, Manning E, Kaminski N. A74-12 An Ex Vivo Model of Induced Lung Aging Reveals Age-dependent Predisposition to Oxidative Stress and Cellular Senescence. American Journal Of Respiratory And Critical Care Medicine 2026, 212: aamag162.5347. DOI: 10.1093/ajrccm/aamag162.5347.Peer-Reviewed Original ResearchPrecision-cut lung slicesProportion of Ki67-positive cellsEx vivo modelKi67-positive cellsAging LungLung diseasePositive cellsCellular senescenceLung slicesAge-related lung diseasePredisposition to oxidative stressProportion of positive cellsSenescence inductionHydrogen peroxide treatmentSenescence marker p21P21-positive cellsCdkn1a (p21Differential gene expressionDose-dependent effectDose-dependent mannerOxidative stress-induced cellular senescenceFetal bovine serumAlveolar simplificationStress-induced cellular senescenceProliferation markersA109-23 The Profibrotic Effect of Mechanical Force on Airway Basal Cells in Pulmonary Fibrosis
Lin L, Salimi M, Du H, Khoury J, Giuseppe D, Alzate K, Szafron J, Manning E, Kaminski N, Ahangari F. A109-23 The Profibrotic Effect of Mechanical Force on Airway Basal Cells in Pulmonary Fibrosis. American Journal Of Respiratory And Critical Care Medicine 2026, 212: aamag162.2423. DOI: 10.1093/ajrccm/aamag162.2423.Peer-Reviewed Original ResearchAirway basal cellsIdiopathic pulmonary fibrosisPulmonary fibrosisProfibrotic effectsBasal cellsBleomycin-treated miceTGF-b1 expressionIdiopathic pulmonary fibrosis progressionAirway epithelial cellsScarring lung diseaseAir-liquid interfaceDifferentiation stateAnnexin V-FITC/PI stainingGene expressionFibrotic remodelingFibrosis progressionNo significant changesHealthy miceAbnormal responseLung diseaseVentilated lungFibrotic genesAlveolar destructionElevated mechanical stressEpithelial cellsC67-06 Aging and Sex Differentially Shape Myeloid Responses and Viral Clearance During Influenza Infection
Kim J, Qiu A, Bain W, Abu Hussein N, Agaronyan K, Suber T, Rizzo A, Evankovich J, Dela Cruz C, Zhao A, Yuan Y, Manning E, Chen L, Sharma L. C67-06 Aging and Sex Differentially Shape Myeloid Responses and Viral Clearance During Influenza Infection. American Journal Of Respiratory And Critical Care Medicine 2026, 212: aamag162.4460. DOI: 10.1093/ajrccm/aamag162.4460.Peer-Reviewed Original ResearchInfluenza infectionViral clearanceSevere influenzaInnate immune response to influenzaRisk of severe influenzaSusceptibility to severe influenzaImmune response to influenzaDefective viral clearanceResponse to influenzaViral transcriptionInflammatory gene signatureFemale C57BL/6 micePersistent pulmonary inflammationWeight lossSex-dependent alterationsAged neutrophilsBody weight lossNS1 protein expressionHigh-risk populationProinflammatory gene expressionCell activity patternsHuman challenge studiesViral RNA quantificationMyeloid populationsPulmonary inflammationC26-11 Biomechanical Modeling of the Evolving Pulmonary Vascular Tree in Animal Models of Pulmonary Hypertension
Jahani F, Manning E, Szafron J. C26-11 Biomechanical Modeling of the Evolving Pulmonary Vascular Tree in Animal Models of Pulmonary Hypertension. American Journal Of Respiratory And Critical Care Medicine 2026, 212: aamag162.5415. DOI: 10.1093/ajrccm/aamag162.5415.Peer-Reviewed Original ResearchG&R modelWall thicknessMass productionExperimental trajectoriesMaterial stiffnessKinetic relationStiffnessBiomechanical stimuliThicknessExperimental researchParameter estimation studiesPulmonary hypertensionModel parametersExperimental datasetsRat datasetsWallWall adaptationParametersHemodynamic featuresPressureAnimal models of pulmonary hypertensionVessel compositionMCT-induced pulmonary vascular remodelingAnimal modelsModel of pulmonary hypertensionThe Interplay of Airway Epithelium and Biomechanical Stress in Pulmonary Fibrosis
Lin L, Khoury J, Zhang G, Szafron J, Manning E, Ahangari F. The Interplay of Airway Epithelium and Biomechanical Stress in Pulmonary Fibrosis. American Journal Of Respiratory Cell And Molecular Biology 2026, aanag066. PMID: 42089315, DOI: 10.1093/ajrcmb/aanag066.Peer-Reviewed Original ResearchIdiopathic pulmonary fibrosisAirway epitheliumPulmonary fibrosisEpithelial responsesEpithelial cellsResponse of airway epithelial cellsPathogenesis of idiopathic pulmonary fibrosisRegulation of lung functionProgression of idiopathic pulmonary fibrosisAirway epithelial cellsProgression of fibrosisAirway epithelial responsesPro-fibrotic responseIndicators of disease onsetFatal lung diseaseEarly indicatorLung fibrosisAbnormal responseLung diseaseDisease onsetLung functionLung tissueFibrosisAirwayTherapeutic interventionsEpidermal growth factor receptor regulates Beclin-1 in hyperoxic acute lung injury
Harris Z, Korde A, Khoury J, Manning E, Stanley G, Shin Y, Mitchell K, von der Schulenburg A, Sun Y, Hu B, Shin H, Joerns J, Clark B, Placek L, Unutmaz D, Moldobaeva A, Sharma L, Sauler M, Rajagopalan G, Zhang X, Wang H, Ghaedi M, Kang M, Koff J. Epidermal growth factor receptor regulates Beclin-1 in hyperoxic acute lung injury. BMJ Open Respiratory Research 2026, 13: e003323. PMID: 41592865, PMCID: PMC12853440, DOI: 10.1136/bmjresp-2025-003323.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorAcute lung injuryHyperoxic ALIHyperoxic acute lung injuryGrowth factor receptorIn vivo effectsLung injuryOxidant-mediated acute lung injuryFactor receptorIntensive care unit mortalityExposure to high oxygenAlveolar type II cellsDelivery of supplemental oxygenPulmonary cell deathLife-saving therapyPluripotent stem cellsType II cellsNormoxia controlSupplemental oxygenUnit mortalityAlveolar epitheliumBeclin-1Cell death pathwaysStem cellsTherapeutic potentialProximal Pulmonary Artery Stiffening as a Biomarker of Cardiopulmonary Aging
De Man R, Cai Z, Doddaballapur P, Guerrera N, Regan A, Lin L, Schwarz E, Justet A, Abu Hussein N, Di Palo J, Cavinato C, Raredon M, Heerdt P, Singh I, Yan X, Kang M, Bruns D, Lee P, Tellides G, Humphrey J, Kaminski N, Ramachandra A, Manning E. Proximal Pulmonary Artery Stiffening as a Biomarker of Cardiopulmonary Aging. Aging Cell 2026, 25: e70383. PMID: 41589414, PMCID: PMC12836046, DOI: 10.1111/acel.70383.Peer-Reviewed Original ResearchConceptsProximal pulmonary arteriesPulmonary arterySmooth muscle cellsPerivascular macrophagesMouse modelMuscle cellsRight ventricle functionMedial smooth muscle cellsPulmonary arterial stiffeningRight ventricleVentricle functionAssociated with senescenceECM turnoverLung functionArterial stiffeningArteryAdventitial fibroblastsMolecular targetsAge-relatedGeroscience hypothesisLungAgeIntercellular signalingMiceMacrophages
Academic Achievements & Community Involvement
Clinical Care
Overview
Edward Manning, MD, PhD, is a specialist in pulmonary and critical care medicine who treats people with lung diseases and breathing challenges. He diagnoses and manages conditions such as persistent shortness of breath and chronic lung disorders, using a range of approaches to improve patients’ well-being.
As an assistant professor of medicine (pulmonary) at Yale School of Medicine, Dr. Manning studies how prolonged low oxygen levels and aging can alter the blood vessels of the lungs and affect heart function. His work aims to develop strategies that support long-term cardiopulmonary health.
Dr. Manning pursued his medical training at Albert Einstein College of Medicine, completed a residency at the Hospital of the University of Pennsylvania, and gained further expertise through a fellowship in pulmonary and critical care medicine at Yale School of Medicine.
Clinical Specialties
Board Certifications
Adult Echocardiography
- Certification Organization
- National Board of Echocardiography
- Original Certification Date
- 2021
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