1999
Dishevelled Proteins Lead to Two Signaling Pathways REGULATION OF LEF-1 AND c-Jun N-TERMINAL KINASE IN MAMMALIAN CELLS*
Li L, Yuan H, Xie W, Mao J, Caruso A, McMahon A, Sussman D, Wu D. Dishevelled Proteins Lead to Two Signaling Pathways REGULATION OF LEF-1 AND c-Jun N-TERMINAL KINASE IN MAMMALIAN CELLS*. Journal Of Biological Chemistry 1999, 274: 129-134. PMID: 9867820, DOI: 10.1074/jbc.274.1.129.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAdaptor Proteins, Signal TransducingAnimalsBeta CateninCalcium-Calmodulin-Dependent Protein KinasesCell Cycle ProteinsCOS CellsCytoskeletal ProteinsDishevelled ProteinsDNA-Binding ProteinsEnzyme ActivationJNK Mitogen-Activated Protein KinasesLymphoid Enhancer-Binding Factor 1MiceMitogen-Activated Protein KinasesPhosphoproteinsSignal TransductionTrans-ActivatorsTranscription FactorsTranscription, GeneticUp-RegulationConceptsJNK activationMammalian cellsT-cell factorSmall G proteinsC-Jun N-terminal kinaseDominant negative mutantBeta-catenin levelsDifferent signaling pathwaysCOS-7 cellsN-terminal kinaseC-Jun NDishevelled proteinsDvl proteinsDEP domainDependent transcriptionNegative mutantPathway regulationKinase activityLEF-1Transcription activitySignaling pathwaysG proteinsNovel pathwayCell factorProtein
1998
Specific Involvement of G Proteins in Regulation of Serum Response Factor-mediated Gene Transcription by Different Receptors*
Mao J, Yuan H, Xie W, Simon M, Wu D. Specific Involvement of G Proteins in Regulation of Serum Response Factor-mediated Gene Transcription by Different Receptors*. Journal Of Biological Chemistry 1998, 273: 27118-27123. PMID: 9765229, DOI: 10.1074/jbc.273.42.27118.Peer-Reviewed Original ResearchMeSH Keywords15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid3T3 CellsAnimalsDNA-Binding ProteinsDrug SynergismEndothelinsGene Expression RegulationGTPase-Activating ProteinsGTP-Binding ProteinsLysophospholipidsMiceNuclear ProteinsProteinsReceptors, Cell SurfaceSerum Response FactorThrombinTranscription, GeneticConceptsThromboxane A2Lysophosphatidic acidType 1 muscarinic receptorG proteinsAlpha1-adrenergic receptorsInterleukin-8 receptorEndogenous G proteinsG protein-coupled receptorsProtein-coupled receptorsEndothelin receptorsMuscarinic receptorsDopamine receptorsSRF activationSerum response factorType 1Gene transcriptionReceptorsDifferent receptorsG protein subunitsSpecific involvementGalphaq/11Gq classCell linesNIH3T3 cellsThrombin
1996
Pertussis Toxin-sensitive Activation of Phospholipase C by the C5a and fMet-Leu-Phe Receptors*
Jiang H, Kuang Y, Wu Y, Smrcka A, Simon M, Wu D. Pertussis Toxin-sensitive Activation of Phospholipase C by the C5a and fMet-Leu-Phe Receptors*. Journal Of Biological Chemistry 1996, 271: 13430-13434. PMID: 8662841, DOI: 10.1074/jbc.271.23.13430.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDCell LineEnzyme ActivationGTP-Binding ProteinsMolecular Sequence DataPertussis ToxinReceptor, Anaphylatoxin C5aReceptors, ComplementReceptors, Formyl PeptideReceptors, ImmunologicReceptors, PeptideSignal TransductionTransfectionType C PhospholipasesVirulence Factors, BordetellaConceptsPLC-beta3PTX-sensitive G proteinsG proteinsFMet-LeuGbetagamma subunitsCOS-7 cellsPhospholipase CPLC-beta2Receptor-mediated PLC activationPTX-sensitive mannerFMLP-mediated activationPertussis toxinPhe receptorCell activationGi proteinsChemoattractant receptorsReceptorsPLC activationC5aBeta2Gq classActivationBeta3Betagamma subunitsSignal transduction pathwaysIdentification of a phospholipase C beta2 region that interacts with Gbeta-gamma.
Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2964-2968. PMID: 8610151, PMCID: PMC39743, DOI: 10.1073/pnas.93.7.2964.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDBlotting, WesternCell LineChlorocebus aethiopsDNA, ComplementaryGlutamic AcidGTP-Binding ProteinsHumansIsoenzymesKidneyMacromolecular SubstancesMammalsMutagenesis, Site-DirectedPoint MutationPolymerase Chain ReactionReceptor, Anaphylatoxin C5aReceptors, ComplementRecombinant Fusion ProteinsRecombinant ProteinsTransfectionType C PhospholipasesConceptsCOS-7 cellsGlutathione S-transferaseGbeta gammaPLC-beta2Fusion proteinBeta gamma subunitsLigand-induced activationAlpha1B-adrenergic receptorMammalian expression plasmidX boxG proteinsY boxExpression plasmidAmino acidsS-transferaseProteinSequenceResiduesCellsActivationBeta2GalphaGbetaC5a receptorCDNA
1995
Selective Coupling of β2-Adrenergic Receptor to Hematopoietic-specific G Proteins (∗)
Wu D, Kuang Y, Wu Y, Jiang H. Selective Coupling of β2-Adrenergic Receptor to Hematopoietic-specific G Proteins (∗). Journal Of Biological Chemistry 1995, 270: 16008-16010. PMID: 7608160, DOI: 10.1074/jbc.270.27.16008.Peer-Reviewed Original ResearchConceptsG alpha 16G alpha 15G alpha 11G alpha qG alpha 14Alpha 14Alpha 11Alpha qPhospholipase CAlpha 15G beta gammaAR agonist isoprenalineAlpha 16Norepinephrine-induced increaseNorepinephrine-induced activationG proteinsActivation of PLCCOS-7 cellsReceptor-mediated activationAccumulation of inositolConcentration-dependent mannerAntagonist propranololCotransfected cellsΒ2-adrenergic receptorAgonist isoprenalineDifferent α1-Adrenergic Receptor Sequences Required for Activating Different Gα Subunits of Gq Class of G Proteins (∗)
Wu D, Jiang H, Simon M. Different α1-Adrenergic Receptor Sequences Required for Activating Different Gα Subunits of Gq Class of G Proteins (∗). Journal Of Biological Chemistry 1995, 270: 9828-9832. PMID: 7730363, DOI: 10.1074/jbc.270.17.9828.Peer-Reviewed Original ResearchConceptsG alpha 16G alpha 14Gq classAlpha 16Receptor sequencesDifferent G alpha subunitsAlpha subunitG proteinsG alpha q/11Different Gα subunitsG alpha subunitsAmino acid stretchAlpha q/11Alpha 14N-terminal segmentThird inner loopGα subunitsBBXXB motifRegulatory proteinsC-terminusN-terminusSpecific mutationsSubunitsProteinSpecific interactions
1993
G Protein-Coupled Signal Transduction Pathways for Interleukin-8
Wu D, LaRosa G, Simon M. G Protein-Coupled Signal Transduction Pathways for Interleukin-8. Science 1993, 261: 101-103. PMID: 8316840, DOI: 10.1126/science.8316840.Peer-Reviewed Original ResearchConceptsIL-8 receptorsInterleukin-8Free beta gamma subunitsPertussis toxin-sensitive G proteinToxin-sensitive G proteinIL-8 actsPhospholipase CSpecific heterotrimeric G proteinsAnti-inflammatory agentsG alpha 15G proteinsG alpha 11G alpha qG alpha 16G protein GiBeta 2 isoformG alpha 14Inflammatory responseAlpha 14Beta gamma subunitsProtein GiAlpha 11Alpha qMajor mediatorAlpha 15Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins.
Wu D, Jiang H, Katz A, Simon M. Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins. Journal Of Biological Chemistry 1993, 268: 3704-3709. PMID: 8381437, DOI: 10.1016/s0021-9258(18)53750-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarbacholCell LineCloning, MolecularEnzyme ActivationGTP-Binding ProteinsInositol PhosphatesKineticsMolecular Sequence DataMutagenesisNorepinephrinePhosphatidylinositol Diacylglycerol-LyasePhosphoric Diester HydrolasesPlasmidsReceptors, MuscarinicRecombinant ProteinsRestriction MappingSequence DeletionTransfectionConceptsG alpha q.PLC-beta 1COS-7 cellsG alpha qAlpha q.Alpha subunitG protein alpha subunitsAlpha qMuscarinic acetylcholine receptor type 1G alpha subunitsPhosphoinositide-specific phospholipase CIntrinsic enzymatic activitySpecific deletionAmino acid sequenceLigand-induced releasePhospholipase C beta 1C beta 1CDNA clonesAcid sequenceC-terminusG proteinsTerminal endDependent activationPhospholipase CAlpha 1C-adrenergic receptor
1992
Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
Katz A, Wu D, Simon M. Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C. Nature 1992, 360: 686-689. PMID: 1465134, DOI: 10.1038/360686a0.Peer-Reviewed Original ResearchConceptsΑ-subunitG proteinsExchange of GDPVitro reconstitution experimentsReceptor-mediated releaseDifferent G proteinsFree GβγPhospholipase C activationGβγ complexG proteins resultsGα subunitsHL-60 cellsGβγ subunitsTransfection assaysPertussis toxinΓ subunitProtein resultsGβγReconstitution experimentsDifferent isoformsSpecific receptorsΒ2 isoformPhospholipase CΒ1 isoformsSubunits