2016
Ultra-High Resolution 3D Imaging of Whole Cells
Huang F, Sirinakis G, Allgeyer ES, Schroeder LK, Duim WC, Kromann EB, Phan T, Rivera-Molina FE, Myers JR, Irnov I, Lessard M, Zhang Y, Handel MA, Jacobs-Wagner C, Lusk CP, Rothman JE, Toomre D, Booth MJ, Bewersdorf J. Ultra-High Resolution 3D Imaging of Whole Cells. Cell 2016, 166: 1028-1040. PMID: 27397506, PMCID: PMC5005454, DOI: 10.1016/j.cell.2016.06.016.Peer-Reviewed Original ResearchConceptsCell biological researchResolution 3D imagingHigh-resolution 3D imagingOptical nanoscopeSuper-resolution microscopyThree-dimensional structureMammalian cellsNuclear poresSynaptonemal complexFluorescence nanoscopyThick samplesThin samplesBiological researchNanoscopyInferior resolutionCellular volumeWhole cellsDepth directionMolecular architecturePractical biological applicationsBiological applicationsComplex molecular architecturesResolutionNanoscopeCells
2007
The Tyrosine Kinase Activity of c-Src Regulates Actin Dynamics and Organization of Podosomes in Osteoclasts
Destaing O, Sanjay A, Itzstein C, Horne WC, Toomre D, De Camilli P, Baron R. The Tyrosine Kinase Activity of c-Src Regulates Actin Dynamics and Organization of Podosomes in Osteoclasts. Molecular Biology Of The Cell 2007, 19: 394-404. PMID: 17978100, PMCID: PMC2174183, DOI: 10.1091/mbc.e07-03-0227.Peer-Reviewed Original ResearchConceptsKinase activityActin cloudDynamic actin-rich structuresSrc family kinase activityDynamics of podosomesActin-rich structuresF-actin coresTyrosine kinase activityAbsence of SrcSrc mutantActin fluxActin dynamicsPodosome numbersEssential regulatorLife spanPodosomesActin polymerizationPodosome organizationF-actinPodosome structuresSrc inhibitorSrcFluorescence recoveryPodosome beltSpecific functions