2016
Quantification of Neural Ethanol and Acetaldehyde Using Headspace GC‐MS
Heit C, Eriksson P, Thompson DC, Charkoftaki G, Fritz KS, Vasiliou V. Quantification of Neural Ethanol and Acetaldehyde Using Headspace GC‐MS. Alcohol Clinical And Experimental Research 2016, 40: 1825-1831. PMID: 27501276, PMCID: PMC5008984, DOI: 10.1111/acer.13156.Peer-Reviewed Original ResearchConceptsAlcohol drinking behaviorEtOH metabolitesMurine brain tissueACh levelsAlcohol consumptionACh concentrationBrain tissueLiver tissueAChAlcohol addictionACh formationBrainDrinking behaviorLow micromolar rangeAlcohol researchTissue analysisTissueNanomolar rangeChromatography-mass spectrometry methodQuantitative assayMass spectrometry methodActive agentsMicromolar rangeSensitive gas chromatography-mass spectrometry methodHeme oxygenase 1 protects ethanol-administered liver tissue in Aldh2 knockout mice
Matsumoto A, Thompson D, Chen Y, Vasiliou V, Kawamoto T, Ichiba M. Heme oxygenase 1 protects ethanol-administered liver tissue in Aldh2 knockout mice. Alcohol 2016, 52: 49-54. PMID: 27139237, DOI: 10.1016/j.alcohol.2016.02.004.Peer-Reviewed Original ResearchConceptsAldh2 knockout miceStress-related proteinsOxidative stress-related proteinsAlanine transaminaseAnti-oxidative proteinsKnockout miceHealthy individualsHepatic tumor necrosis factor alphaLiver tissueProtective factorsTumor necrosis factor alphaSerum alanine transaminaseRecent epidemiological studiesNecrosis factor alphaWild-type miceHeme oxygenase-1Cytochrome P450 2E1ALDH2 proteinProteinAldehyde dehydrogenase 2 geneHepatic malondialdehydeMechanistic explanationInflammatory cytokinesEthanol administrationMechanistic hypothesesAldehyde Dehydrogenase 1B1 as a Modulator of Pancreatic Adenocarcinoma
Singh S, Arcaroli JJ, Orlicky DJ, Chen Y, Messersmith WA, Bagby S, Purkey A, Quackenbush KS, Thompson DC, Vasiliou V. Aldehyde Dehydrogenase 1B1 as a Modulator of Pancreatic Adenocarcinoma. Pancreas 2016, 45: 117-122. PMID: 26566217, PMCID: PMC5175203, DOI: 10.1097/mpa.0000000000000542.Peer-Reviewed Original ResearchMeSH KeywordsAldehyde DehydrogenaseAldehyde Dehydrogenase 1 FamilyAldehyde Dehydrogenase, MitochondrialAnimalsBiomarkers, TumorCarcinoma, Pancreatic DuctalCell Line, TumorCell ProliferationFemaleGene Expression Regulation, EnzymologicGene Expression Regulation, NeoplasticHumansImmunohistochemistryMice, NudeNeoplasm InvasivenessPancreatic NeoplasmsRNA InterferenceSignal TransductionTissue Array AnalysisTransfectionTumor BurdenUp-RegulationConceptsALDH1B1 expressionPancreatic cancerPancreatic adenocarcinomaTissue microarrayHuman pancreatic cancer cell linesPancreatic cancer cell linesPancreatic cancer patientsPancreatic ductal carcinomaHuman pancreatic cancerAldehyde dehydrogenase 1B1Potential modulatory rolePancreatic cancer cellsNormal human pancreasCell linesCancer cell linesDuctal carcinomaCancer patientsModulatory roleHuman pancreasGlandular cellsTumor cellsProtein expressionCancer cellsGreater expressionAdenocarcinoma
2012
ALDH1A Isozymes are Markers of Human Melanoma Stem Cells and Potential Therapeutic Targets
Luo Y, Dallaglio K, Chen Y, Robinson WA, Robinson SE, McCarter MD, Wang J, Gonzalez R, Thompson DC, Norris DA, Roop DR, Vasiliou V, Fujita M. ALDH1A Isozymes are Markers of Human Melanoma Stem Cells and Potential Therapeutic Targets. Stem Cells 2012, 30: 2100-2113. PMID: 22887839, PMCID: PMC3448863, DOI: 10.1002/stem.1193.Peer-Reviewed Original ResearchMeSH KeywordsAldehyde DehydrogenaseAldehyde Dehydrogenase 1 FamilyAldehyde OxidoreductasesAnimalsApoptosisCell Transformation, NeoplasticDacarbazineDrug Resistance, NeoplasmFemaleGene Expression Regulation, NeoplasticGene SilencingHumansIsoenzymesMelanomaMiceMice, Inbred NODMice, SCIDNeoplasm TransplantationNeoplastic Stem CellsResponse ElementsRetinal DehydrogenaseRNA, Small InterferingSkin NeoplasmsTemozolomideTretinoinConceptsCancer stem cellsPositive melanoma cellsMelanoma cellsTherapeutic targetBiomarkers of CSCsHuman melanomaPatient-derived tumor specimensMelanoma cancer stem cellsNOD/SCID miceALDH-negative cellsHigh aldehyde dehydrogenase (ALDH) activityALDH isozymesNonobese diabetic/Potential therapeutic targetDrug-induced cell deathAttractive therapeutic targetNew molecular targetsHuman melanoma cellsStem cellsMelanoma stem cellsAldehyde dehydrogenase activityHuman melanoma stem cellsNSG miceCell cycle arrestImmunodeficiency miceCharacterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures
Saw YT, Yang J, Ng SK, Liu S, Singh S, Singh M, Welch WR, Tsuda H, Fong WP, Thompson D, Vasiliou V, Berkowitz RS, Ng SW. Characterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures. BMC Cancer 2012, 12: 329. PMID: 22852552, PMCID: PMC3458927, DOI: 10.1186/1471-2407-12-329.Peer-Reviewed Original ResearchConceptsClear cell tumorsWestern blot analysisCell tumorsOvarian tissueALDH isozymesCancer cellsClear cell subtypeSphere cultureOvarian cancer tissuesQuantitative reverse transcription polymerase chain reactionNormal ovarian tissuesOvarian epithelial tumorsReverse transcription-polymerase chain reactionHuman ovarian cancerOvarian tumor tissuesTranscription-polymerase chain reactionOvarian cancer cellsBlot analysisHuman cancer stem cellsCancer stem cellsOvarian cancer pathophysiologyMRNA expression levelsResultsImmunohistochemical stainingCancer cell linesMucinous tumors
1990
Nonadrenergic bronchodilation induced by high concentrations of sulfur dioxide
Thompson D, Szarek J, Altiere R, Diamond L. Nonadrenergic bronchodilation induced by high concentrations of sulfur dioxide. Journal Of Applied Physiology 1990, 69: 1786-1791. PMID: 2272972, DOI: 10.1152/jappl.1990.69.5.1786.Peer-Reviewed Original ResearchConceptsBronchodilator responseNonadrenergic noncholinergicGanglionic blocking agent hexamethoniumAirway smooth muscle toneConcentration-dependent bronchodilationNANC inhibitory transmitterDynamic lung complianceCyclooxygenase inhibitor indomethacinSmooth muscle toneAgent hexamethoniumBronchoconstrictive stimuliNonadrenergic bronchodilationBronchomotor toneIntravenous atropineAutonomic reflexesInhibitory transmitterLung complianceLung resistanceContinuous infusionFeline airwaysMuscle toneCromolyn sodiumInhibitor indomethacinInhibitory systemBronchodilationNICOTINIC AGONIST MODULATION OF FELINE BRONCHOMOTOR TONE
Thompson D, Altiere R, Diamond L. NICOTINIC AGONIST MODULATION OF FELINE BRONCHOMOTOR TONE. Clinical And Experimental Pharmacology And Physiology 1990, 17: 83-97. PMID: 2328544, DOI: 10.1111/j.1440-1681.1990.tb01291.x.Peer-Reviewed Original Research
1988
The effect of epithelium removal on non-adrenergic, non-cholinergic inhibitory responses in the isolated central airways of the cat and guinea pig
Thompson D, Wells J, Altiere R, Diamond L. The effect of epithelium removal on non-adrenergic, non-cholinergic inhibitory responses in the isolated central airways of the cat and guinea pig. European Journal Of Pharmacology 1988, 145: 231-237. PMID: 3350044, DOI: 10.1016/0014-2999(88)90237-3.Peer-Reviewed Original ResearchConceptsElectrical field stimulationEpithelium removalField stimulationHilar bronchusNon-cholinergic inhibitory responsesTissue sensitivityRelaxation responseNANC relaxation responsesGuinea pig tracheaBronchial sensitivityAirway preparationsContractile responseContractile actionContractile agonistsCentral airwaysPig tracheaAirway epitheliumInhibitory responsesPharmacological agonistsFeline tracheaGuinea pigsBronchusStimulationAgonistsTrachea