2014
Calcium sensitive ring-like oligomers formed by synaptotagmin
Wang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV, Rothman JE. Calcium sensitive ring-like oligomers formed by synaptotagmin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 13966-13971. PMID: 25201968, PMCID: PMC4183308, DOI: 10.1073/pnas.1415849111.Peer-Reviewed Original ResearchMeSH KeywordsCalciumHumansLipid BilayersMultiprotein ComplexesProtein Structure, TertiarySNARE ProteinsSynaptotagmin IConceptsSynaptic vesicle protein Synaptotagmin 1Cytosolic domainSoluble N-ethylmaleimide-sensitive factorN-ethylmaleimide-sensitive factorMembrane fusion machineryReceptor complex assemblyRing-like oligomersFusion machineryC2 domainComplex assemblySynaptotagmin-1Helical reconstructionFusion proceedsNovel mechanismStructural mechanismsLipid monolayersNeurotransmitter releaseAbsence of calciumPhysiological concentrationsRing formationPresence of calciumFree calcium ionsSynaptotagminCalcium influxCircular arrangementHigh-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation
Shang Z, Zhou K, Xu C, Csencsits R, Cochran JC, Sindelar CV. High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation. ELife 2014, 3: e04686. PMID: 25415053, PMCID: PMC4383081, DOI: 10.7554/elife.04686.Peer-Reviewed Original Research
2012
Optimal noise reduction in 3D reconstructions of single particles using a volume-normalized filter
Sindelar CV, Grigorieff N. Optimal noise reduction in 3D reconstructions of single particles using a volume-normalized filter. Journal Of Structural Biology 2012, 180: 26-38. PMID: 22613568, PMCID: PMC3498508, DOI: 10.1016/j.jsb.2012.05.005.Peer-Reviewed Original ResearchConceptsParticle mapsOptimal noise reductionWiener filterError reduction schemeConventional Wiener filterNoise reductionNovel filterInversion methodNew filterHigh noise levelsParticle densityParticle volumeFilterParticlesNoise ratioNoise levelTheoretical analysisSingle particleAccurate implementationSpectral signalsSSNRInterior regionImage dataSignalsFourier inversion method