2018
Labeling for Quantitative Comparison of Imaging Measurements in Vitro and in Cells
Davis CM, Gruebele M. Labeling for Quantitative Comparison of Imaging Measurements in Vitro and in Cells. Biochemistry 2018, 57: 1929-1938. PMID: 29546761, DOI: 10.1021/acs.biochem.8b00141.Peer-Reviewed Original ResearchConceptsCell biologyComplex dissociation constantsProtein structureBiophysical experimentsModification sitesCell proteinsSuch measurementsQualitative imagingImaging measurementsBiologyCellsPowerful techniqueMeasurementsQuantitative measurementsTarget moleculesDissociation constantsRNAProteinQuantitative comparisonVitroLocalizationConstantsLabelingTarget
2017
Dual time-resolved temperature-jump fluorescence and infrared spectroscopy for the study of fast protein dynamics
Davis CM, Reddish MJ, Dyer RB. Dual time-resolved temperature-jump fluorescence and infrared spectroscopy for the study of fast protein dynamics. Spectrochimica Acta Part A Molecular And Biomolecular Spectroscopy 2017, 178: 185-191. PMID: 28189834, PMCID: PMC5346054, DOI: 10.1016/j.saa.2017.01.069.Peer-Reviewed Original ResearchConceptsQuantum cascade lasersTime-resolved IRYAG laserTunable quantum cascade laserFluorescence spectroscopyProtein dynamicsAbsorbance detection limitCascade lasersSapphire laserComplex folding mechanismsLaserIR frequenciesOverall fluorescence intensityFluorescence spectrometerSpectroscopyIR spectrometerFluorescence measurementsFluorescence sensitivityHigh sensitivityT-jumpSpectrometerFast protein dynamicsFolding mechanismPowerful techniqueFluorescence