2013
Differential Expression of HPV16 L2 Gene in Cervical Cancers Harboring Episomal HPV16 Genomes: Influence of Synonymous and Non-Coding Region Variations
Mandal P, Bhattacharjee B, Ghosh D, Mondal N, Chowdhury R, Roy S, Sengupta S. Differential Expression of HPV16 L2 Gene in Cervical Cancers Harboring Episomal HPV16 Genomes: Influence of Synonymous and Non-Coding Region Variations. PLOS ONE 2013, 8: e65647. PMID: 23762404, PMCID: PMC3675152, DOI: 10.1371/journal.pone.0065647.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedCapsid ProteinsCarcinoma, Squamous CellFemaleGene DosageGene Expression Regulation, ViralGenome, ViralHost-Pathogen InteractionsHuman papillomavirus 16HumansMicroRNAsMiddle AgedOncogene Proteins, ViralOpen Reading FramesPapillomavirus InfectionsPlasmidsPolymorphism, GeneticPromoter Regions, GeneticRNA, MessengerSequence Analysis, DNAUterine Cervical NeoplasmsConceptsNon-coding regionsHPV16 genomeSynonymous variationNon-malignant samplesHuman codonsL2 genesWhole-genome sequence analysisShort non-coding regionHPV16 isolatesGenome sequence analysisMiRNA binding sitesWeak promoter activityHsa-mir-548Gene copy numberCaCx casesEpisomal HPV16 genomesSequence variationCoding regionSequence analysisGenomeNon-codingViral genomeEpisomal viral genomesCopy numberL2 ORF
2010
Association of viral load with HPV16 positive cervical cancer pathogenesis: Causal relevance in isolates harboring intact viral E2 gene
Das D, Bhattacharjee B, Sen S, Mukhopadhyay I, Sengupta S. Association of viral load with HPV16 positive cervical cancer pathogenesis: Causal relevance in isolates harboring intact viral E2 gene. Virology 2010, 402: 197-202. PMID: 20394955, DOI: 10.1016/j.virol.2010.03.030.Peer-Reviewed Original ResearchMeSH KeywordsAdultDNA, ViralDNA-Binding ProteinsFemaleHuman papillomavirus 16HumansMiddle AgedOncogene Proteins, ViralPapillomavirus InfectionsUterine Cervical NeoplasmsViral LoadYoung AdultConceptsViral loadE2 statusAssociation of viral loadMedian viral loadViral loads compared to controlsAge of subjectsCervical cancer pathogenesisViral E2 geneCompared to controlsCervical cancerLogistic regression modelsCancer pathogenesisTaqMan assayLower ageCaCxHPV16E2 genePathogenesisAgeIntact E2Regression modelsStatusGenomic DNAAssociationCancer
2008
Characterization of sequence variations within HPV16 isolates among Indian women: Prediction of causal role of rare non-synonymous variations within intact isolates in cervical cancer pathogenesis
Bhattacharjee B, Mandal N, Roy S, Sengupta S. Characterization of sequence variations within HPV16 isolates among Indian women: Prediction of causal role of rare non-synonymous variations within intact isolates in cervical cancer pathogenesis. Virology 2008, 377: 143-150. PMID: 18495198, DOI: 10.1016/j.virol.2008.04.007.Peer-Reviewed Original ResearchConceptsNon-synonymous changesCharacterization of sequence variationsPolymorphic variationDeleterious to protein functionHPV16 isolatesNon-synonymous variationsRare non-synonymous variationsDeleterious variationHaplotype backgroundNucleotide variationsSIFTS databaseDeleterious mutationsSequence variationSequence variantsProtein functionHPV16 genomeHaplotype analysisHaplotypesFunctional validationCervical cancer pathogenesisE-12IsolatesCaCx developmentCancer pathogenesisProductive infection
2006
CpG methylation of HPV 16 LCR at E2 binding site proximal to P97 is associated with cervical cancer in presence of intact E2
Bhattacharjee B, Sengupta S. CpG methylation of HPV 16 LCR at E2 binding site proximal to P97 is associated with cervical cancer in presence of intact E2. Virology 2006, 354: 280-285. PMID: 16905170, DOI: 10.1016/j.virol.2006.06.018.Peer-Reviewed Original ResearchConceptsLong control regionE2 binding sitesP97 promoterCervical cancerNormal controlsAssociated with cervical cancerCytologically normal controlsIntact E2 geneHuman papillomavirus type 16Binding sitesMspI restriction digestionRegulate transcriptionRepressor activityHPV-16CaCx samplesCpG methylationControl regionIntact E2Restriction digestionE7 genesType 16E2 geneCaCxMalignant samplesSite methylation