2009
Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1
Esemuede N, Lee T, Maier KG, Sumpio BE, Gahtan V. Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1. Journal Of Surgical Research 2009, 168: 149-154. PMID: 20338582, DOI: 10.1016/j.jss.2009.11.728.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell ProliferationCells, CulturedChemotaxisDose-Response Relationship, DrugHydroxymethylglutaryl-CoA Reductase InhibitorsLovastatinMevalonic AcidModels, AnimalMonomeric GTP-Binding ProteinsMuscle, Smooth, VascularRas ProteinsSignal TransductionThrombospondin 1Tunica IntimaTunica MediaConceptsTSP-1-induced chemotaxisGeranylgeranyl transferase inhibitorSerum-free mediumThrombospondin-1HMG-CoA reductase inhibitorsVascular smooth muscle cell migrationSmooth muscle cell migrationTransferase inhibitorsEffect of lovastatinG proteinsMuscle cell migrationRas activationRho-kinase inhibitorCell migrationPost-hoc testingFarnesyl transferase inhibitorsCholesterol loweringLovastatin doseVascular restenosisIntimal hyperplasiaBoyden chamberPleiotropic propertiesReductase inhibitorsWestern blotInhibition of Ras
2008
The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration
Fuse S, Esemuede N, Yamaguchi M, Maier KG, Nesselroth SM, Sumpio BE, Gahtan V. The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration. Surgery 2008, 144: 86-92. PMID: 18571589, DOI: 10.1016/j.surg.2008.03.028.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsTSP-1-induced chemotaxisExtracellular signal-regulated kinase 1/2Pertussis toxinSerum-free mediumCholera toxinThrombospondin-1CAMP levelsP38 activationG proteinsVascular smooth muscle cell migrationQuiescent vascular smooth muscle cellsSmooth muscle cell migrationCyclic adenosine monophosphate levelsSmooth muscle cellsIntracellular cyclic adenosine monophosphate (cAMP) levelsMuscle cell migrationTSP-1 receptorAdenosine monophosphate levelsSignal-regulated kinase 1/2Levels of cAMPArterial lesionsVascular injuryVSMC chemotaxisMicrochemotaxis chamberThrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase
Wang XJ, Maier K, Fuse S, Willis AI, Olson E, Nesselroth S, Sumpio BE, Gahtan V. Thrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase. Vascular And Endovascular Surgery 2008, 42: 256-262. PMID: 18319354, DOI: 10.1177/1538574408314440.Peer-Reviewed Original ResearchConceptsSmooth muscle cell migrationVascular smooth muscle cell migrationMuscle cell migrationSmooth muscle cellsMuscle cellsCell migrationThrombospondin-1
2005
Nicotine induces mitogen-activated protein kinase dependent vascular smooth muscle cell migration
Di Luozzo G, Pradhan S, Dhadwal AK, Chen A, Ueno H, Sumpio BE. Nicotine induces mitogen-activated protein kinase dependent vascular smooth muscle cell migration. Atherosclerosis 2005, 178: 271-277. PMID: 15694934, DOI: 10.1016/j.atherosclerosis.2004.09.017.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cell migrationSmooth muscle cell migrationMuscle cell migrationInhibitors of p38VSMC migrationMitogen-activated protein kinaseEndothelial cellsDevelopment of atherosclerosisEffects of nicotineCell migrationWestern blotting methodInjury modelVascular endotheliumCigarette smokeBoyden chamber chemotaxis assaysAortic VSMCsChemotactic effectMAPKs p38NicotineChemoattractant moleculesChemotaxis assaysCellular mechanismsUltrastructural changesVSMCP44/42 activationShear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α
Dardik A, Yamashita A, Aziz F, Asada H, Sumpio BE. Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α. Journal Of Vascular Surgery 2005, 41: 321-331. PMID: 15768016, DOI: 10.1016/j.jvs.2004.11.016.Peer-Reviewed Original ResearchConceptsSignal transduction pathwaysPlatelet-derived growth factorSMC chemotaxisCell signal transduction pathwaysMitogen-activated protein kinase pathwaySmooth muscle cell chemotaxisERK1/2 phosphorylationPDGF-BBExtracellular signal-regulated protein kinase 1/2Pathway inhibitor PD98059Protein kinase pathwayEndothelial cellsERK1/2 signal transduction pathwayProtein kinase 1/2Vascular smooth muscle cell migrationBovine aortic endothelial cellsKinase pathwayInhibitor PD98059Smooth muscle cell migrationHemodynamic forcesKinase 1/2Platelet-derived growthMuscle cell migrationWestern blot analysisTarget pathways
2004
Vascular Smooth Muscle Cell Migration: Current Research and Clinical Implications
Willis AI, Pierre-Paul D, Sumpio BE, Gahtan V. Vascular Smooth Muscle Cell Migration: Current Research and Clinical Implications. Vascular And Endovascular Surgery 2004, 38: 11-23. PMID: 14760473, DOI: 10.1177/153857440403800102.Peer-Reviewed Original ResearchConceptsVSMC migrationVascular smooth muscle cell migrationSmooth muscle cell migrationPotential therapeutic approachMuscle cell migrationDiabetes mellitusEndothelial injuryTherapeutic approachesIntimal hyperplasiaClinical implicationsMajor causeDevelopment of strategiesInjuryCell migrationMultiple stimuliHypertensionMellitusHyperlipidemiaMorbiditySmokingHyperplasiaMortality
2003
Thrombospondin-1 induces matrix metalloproteinase-2 activation in vascular smooth muscle cells1 1Competition of interest: none.
Lee T, Esemuede N, Sumpio BE, Gahtan V. Thrombospondin-1 induces matrix metalloproteinase-2 activation in vascular smooth muscle cells1 1Competition of interest: none. Journal Of Vascular Surgery 2003, 38: 147-154. PMID: 12844104, DOI: 10.1016/s0741-5214(02)75468-2.Peer-Reviewed Original ResearchConceptsThrombospondin-1TSP-1-induced VSMC migrationVSMC migrationCell migrationMatrix metalloproteinase-2MMP2 activationMatrix barrierSerum-free mediumNorthern blot analysisVascular smooth muscle cell migrationCellular processesExtracellular matrix glycoproteinMMP2 activityPosttranslational mechanismsMatrix metalloproteinase-2 activationMetalloproteinase-2 activationSmooth muscle cell migrationMuscle cell migrationInvasion assaysStrong binding activityBinding activityECM degradationMatrix glycoproteinRNA expressionBlot analysis
2000
Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis
Willis A, Fuse S, Wang X, Chen E, Tuszynski G, Sumpio B, Gahtan V. Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis. Journal Of Vascular Surgery 2000, 31: 1160-1167. PMID: 10842153, DOI: 10.1067/mva.2000.106489.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell CountCell DivisionCells, CulturedChemotaxisChromonesEnzyme InhibitorsExtracellular Matrix ProteinsFibronectinsHyperplasiaIndolesMaleimidesMorpholinesMuscle, Smooth, VascularPhosphoinositide-3 Kinase InhibitorsProtein Kinase CSignal TransductionStatistics as TopicThrombospondin 1Tunica IntimaVitronectinConceptsProtein kinase CVSMC migrationLY-294002Thrombospondin-1ECM proteinsKinase CSmooth muscle cell chemotaxisSerum-free mediumCellular signaling pathwaysInhibition of phosphatidylinositolVascular smooth muscle cell chemotaxisVascular smooth muscle cell migrationPI3K inhibitorsSmooth muscle cell migrationSignaling pathwaysMuscle cell migrationBisindolylmaleimide ICell migrationPKC inhibitorGF 109203XExtracellular matrix depositionTop wellsK inhibitorsAortic VSMCsCell chemotaxis