2004
Molecular and biological effects of hemodynamics on vascular cells.
Pradhan S, Sumpio B. Molecular and biological effects of hemodynamics on vascular cells. Frontiers In Bioscience-Landmark 2004, 9: 3276-85. PMID: 15353357, DOI: 10.2741/1480.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtherosclerosisCell MovementCell ProliferationCells, CulturedCyclic AMPDiglyceridesDisease ProgressionEndothelial CellsExtracellular MatrixHemodynamicsHumansInositol 1,4,5-TrisphosphateIntercellular Adhesion Molecule-1Plasminogen ActivatorsProtein Kinase CRisk FactorsStress, MechanicalConceptsEndothelial cellsSignificant atherosclerotic plaqueSystemic risk factorsTissue plasminogen activatorCellular adhesion moleculesCurrent pertinent literatureCultured endothelial cellsRisk factorsSecond messenger systemsSignificant lesionsProtein kinase C pathwayICAM-1Atherosclerosis formationAtherosclerotic plaquesVascular anatomyKinase C pathwayNitric oxideVascular cellsPlasminogen activatorAdhesion moleculesBlood vesselsVascular biologyMessenger systemsVessel wallPertinent literature17β-Estradiol Exposure Leads to Activation of ERK and p38 but Not JNK in Vascular Endothelial Cells
Knox R, Chen E, Wang X, Jeffries B, Yamaguchi M, Kim S, Sumpio B. 17β-Estradiol Exposure Leads to Activation of ERK and p38 but Not JNK in Vascular Endothelial Cells. International Journal Of Angiology 2004, 13: 67-70. DOI: 10.1007/s00547-004-1068-9.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseVascular endothelial cellsBovine aortic endothelial cellsEndothelial cellsNitric oxideNitric oxide synthaseEndothelial NO productionAnti-phosphospecific antibodiesEndothelial cell productionAortic endothelial cellsRapid activationAtheroprotective effectsActivation of ERKOxide synthaseFetal calf serumE2 exposureNO productionENOS activationActivity of JNKEstrogenBiphasic patternP38 phosphorylationMonophasic mannerTotal ERKERK phosphorylation17β-Estradiol exposure leads to activation of ERK and p38 but not JNK in vascular endothelial cells
Knox R, Chen E, Wang X, Jeffries B, Yamaguchi M, Kim S, Sumpio B. 17β-Estradiol exposure leads to activation of ERK and p38 but not JNK in vascular endothelial cells. International Journal Of Angiology 2004, 67-70. DOI: 10.1055/s-0031-1276450.Peer-Reviewed Original ResearchEndothelial nitric oxide synthaseVascular endothelial cellsBovine aortic endothelial cellsEndothelial cellsNitric oxideNitric oxide synthaseEndothelial NO productionAnti-phosphospecific antibodiesEndothelial cell productionAortic endothelial cellsRapid activationAtheroprotective effectsActivation of ERKOxide synthaseFetal calf serumE2 exposureNO productionENOS activationActivity of JNKEstrogenBiphasic patternP38 phosphorylationMonophasic mannerTotal ERKERK phosphorylation
1997
Gene Regulation by Mechanical Forces
Oluwole BO, Du W, Mills I, Sumpio BE. Gene Regulation by Mechanical Forces. Endothelium 1997, 5: 85-93. PMID: 9237042, DOI: 10.3109/10623329709079866.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Adhesion MoleculesCell Culture TechniquesCells, CulturedEndothelinsEndothelium, VascularEpoprostenolFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression RegulationHemorheologyHumansMechanoreceptorsMembranes, ArtificialNitric OxideProtein-Tyrosine KinasesSignal TransductionStress, MechanicalTissue Plasminogen ActivatorTranscription FactorsTranscription, GeneticVacuumConceptsEndothelial cellsEndothelial cell functionCyclic strainVasoactive mediatorsSecond messenger cascadesNitric oxideBlood vesselsMechanical forcesCell functionMechanical deformationFlow of bloodMessenger cascadesLuminal surfaceSpecific response elementsPromoter geneResponse elementExternal forcesMediatorsCytoskeletal changesCellsForceProstacyclinExpressionTranscription levels
1995
Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells.
Awolesi MA, Sessa WC, Sumpio BE. Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells. Journal Of Clinical Investigation 1995, 96: 1449-1454. PMID: 7544806, PMCID: PMC185768, DOI: 10.1172/jci118181.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAortic endothelial cellsNitric oxide synthaseBovine aortic endothelial cellsCultured bovine aortic endothelial cellsEndothelial cellsOxide synthaseENOS transcriptsRegulation of eNOSENOS gene expressionBlot analysisHemodynamic forcesWestern blot analysisENOS levelsCycles/minImmunohistochemical stainingNitric oxideProtein levelsNorthern blot analysisNuclear runoff transcription assaysVivoCellsRunoff transcription assaysCytosolic RNAExpression
1994
Cyclic strain increases endothelial nitric oxide synthase activity.
Awolesi MA, Widmann MD, Sessa WC, Sumpio BE. Cyclic strain increases endothelial nitric oxide synthase activity. Surgery 1994, 116: 439-44; discussion 444-5. PMID: 7519368.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseRegulation of eNOSCultured bovine aortic endothelial cellsENOS activityAortic endothelial cellsEndothelial cellsBovine aortic endothelial cellsEndothelial nitric oxide synthase activityNitric oxideNitric oxide synthase activityOxide synthase activityNitric oxide synthaseNitric oxide productionHemodynamic forcesRelaxing factorsGreiss reactionCycles/minOxide synthaseENOS geneMedium of ECOxide productionCycles/minuteFunctional activityStrain regimensSynthase activity