2016
Chitinase 3-Like 1 (Chil1) Regulates Survival and Macrophage-Mediated Interleukin-1β and Tumor Necrosis Factor Alpha during Pseudomonas aeruginosa Pneumonia
Marion CR, Wang J, Sharma L, Losier A, Lui W, Andrews N, Elias JA, Kazmierczak BI, Roy CR, Dela Cruz CS. Chitinase 3-Like 1 (Chil1) Regulates Survival and Macrophage-Mediated Interleukin-1β and Tumor Necrosis Factor Alpha during Pseudomonas aeruginosa Pneumonia. Infection And Immunity 2016, 84: 2094-2104. PMID: 27141083, PMCID: PMC4936356, DOI: 10.1128/iai.00055-16.Peer-Reviewed Original ResearchConceptsBone marrow-derived macrophagesTumor necrosis factor alphaExcessive IL-1β productionNecrosis factor alphaIL-1β productionWT miceAeruginosa pneumoniaFactor alphaChitinase 3Lung parenchymal damageHospital-acquired pneumoniaIL-13 receptor α2Pseudomonas aeruginosa pneumoniaP. aeruginosa pneumoniaDecreased survival timeStreptococcus pneumoniae infectionHost inflammatory responseP. aeruginosa infectionInterleukin-1β productionMarrow-derived macrophagesHost tissue damageP. aeruginosaHost tolerancePneumoniae infectionParenchymal damage
2014
Distinct Contributions of Interleukin-1α (IL-1α) and IL-1β to Innate Immune Recognition of Pseudomonas aeruginosa in the Lung
Al Moussawi K, Kazmierczak BI. Distinct Contributions of Interleukin-1α (IL-1α) and IL-1β to Innate Immune Recognition of Pseudomonas aeruginosa in the Lung. Infection And Immunity 2014, 82: 4204-4211. PMID: 25069982, PMCID: PMC4187872, DOI: 10.1128/iai.02218-14.Peer-Reviewed Original ResearchConceptsIL-1βIL-1αImmune responseCaspase-1Deficient miceExtracellular Toll-like receptorsNLRC4 inflammasomeStrong innate immune responseIL-1β signalRecruitment of neutrophilsToll-like receptorsInnate immune recognitionIL-1 receptorP. aeruginosa isolatesInnate immune responseP. aeruginosa infectionSite of infectionIL-1α secretionT3SS effectorsWild-type animalsNegative bacterial isolatesPseudomonas aeruginosaNeutrophilic responseAcute infectionNeutrophil recruitmentCheating by type 3 secretion system-negative Pseudomonas aeruginosa during pulmonary infection
Czechowska K, McKeithen-Mead S, Al Moussawi K, Kazmierczak BI. Cheating by type 3 secretion system-negative Pseudomonas aeruginosa during pulmonary infection. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 7801-7806. PMID: 24821799, PMCID: PMC4040582, DOI: 10.1073/pnas.1400782111.Peer-Reviewed Original ResearchConceptsT3SS-inducing conditionsOpportunistic pathogen Pseudomonas aeruginosaType 3 secretion systemPathogen Pseudomonas aeruginosaWT P. aeruginosaPositive bacteriaFitness advantageDefective mutantsRelative fitnessSecretion systemBacterial virulenceSelective advantageMyD88 knockout miceNeutrophil-depleted animalsVitro growth rateMutantsInnate immune cellsPseudomonas aeruginosaP. aeruginosa infectionCompetition experimentsAcute pneumonia modelBacteriaFitnessPulmonary infectionImmunocompetent hosts
2012
The Ability of Virulence Factor Expression by Pseudomonas aeruginosa to Predict Clinical Disease in Hospitalized Patients
Ledizet M, Murray TS, Puttagunta S, Slade MD, Quagliarello VJ, Kazmierczak BI. The Ability of Virulence Factor Expression by Pseudomonas aeruginosa to Predict Clinical Disease in Hospitalized Patients. PLOS ONE 2012, 7: e49578. PMID: 23152923, PMCID: PMC3495863, DOI: 10.1371/journal.pone.0049578.Peer-Reviewed Original ResearchConceptsP. aeruginosa infectionAeruginosa infectionBacterial factorsHospitalized patientsUrinary tractPositive P. aeruginosa culturesP. aeruginosaUrinary tract cathetersP. aeruginosa isolatesLogistic regression modelsPseudomonas aeruginosaProspective cohortDiabetes mellitusSubgroup analysisClinical dataTreatment decisionsClinical diseaseAeruginosa isolatesAnimal modelsPatientsClinical sitesFactor expressionInfectionHost factorsP. aeruginosa cultures
2011
Airway Epithelial MyD88 Restores Control of Pseudomonas aeruginosa Murine Infection via an IL-1–Dependent Pathway
Mijares LA, Wangdi T, Sokol C, Homer R, Medzhitov R, Kazmierczak BI. Airway Epithelial MyD88 Restores Control of Pseudomonas aeruginosa Murine Infection via an IL-1–Dependent Pathway. The Journal Of Immunology 2011, 186: 7080-7088. PMID: 21572023, PMCID: PMC3110630, DOI: 10.4049/jimmunol.1003687.Peer-Reviewed Original ResearchConceptsInnate immune responseImmune responseMyD88-dependent innate immune responsesIL-1-dependent pathwayBone marrow chimeric miceProtective innate immune responseP. aeruginosaNovel transgenic mouse modelVentilator-associated pneumoniaIL-1R signalingTransgenic mouse modelP. aeruginosa infectionEpithelial cell responsesRadio-resistant cellsIntranasal infectionMyD88 expressionMultiple TLR pathwaysMyD88 functionAeruginosa infectionMouse modelTLR pathwayMurine infectionChimeric miceCell responsesInfection