2020
A Screen for Antibiotic Resistance Determinants Reveals a Fitness Cost of the Flagellum in Pseudomonas aeruginosa
Rundell EA, Commodore N, Goodman AL, Kazmierczak BI. A Screen for Antibiotic Resistance Determinants Reveals a Fitness Cost of the Flagellum in Pseudomonas aeruginosa. Journal Of Bacteriology 2020, 202: 10.1128/jb.00682-19. PMID: 31871033, PMCID: PMC7043666, DOI: 10.1128/jb.00682-19.Peer-Reviewed Original ResearchConceptsFlagellar assemblyFitness advantageFitness costsCell envelopeOuter membrane barrier functionOuter membrane biogenesisUnbiased high-throughput approachOuter membrane barrierMembrane barrier functionHigh-throughput approachMembrane biogenesisGram-negative bacteriaFlagellar functionInsertion sequencingOuter membraneGene productsPresence of linezolidPresence of antibioticsAntibiotic resistance determinantsMembrane barrierAntibiotic entryFitnessEfflux pumpsGlycopeptide antibiotic vancomycinGenes
2014
Cheating by type 3 secretion system-negative Pseudomonas aeruginosa during pulmonary infection
Czechowska K, McKeithen-Mead S, Al Moussawi K, Kazmierczak BI. Cheating by type 3 secretion system-negative Pseudomonas aeruginosa during pulmonary infection. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 7801-7806. PMID: 24821799, PMCID: PMC4040582, DOI: 10.1073/pnas.1400782111.Peer-Reviewed Original ResearchConceptsT3SS-inducing conditionsOpportunistic pathogen Pseudomonas aeruginosaType 3 secretion systemPathogen Pseudomonas aeruginosaWT P. aeruginosaPositive bacteriaFitness advantageDefective mutantsRelative fitnessSecretion systemBacterial virulenceSelective advantageMyD88 knockout miceNeutrophil-depleted animalsVitro growth rateMutantsInnate immune cellsPseudomonas aeruginosaP. aeruginosa infectionCompetition experimentsAcute pneumonia modelBacteriaFitnessPulmonary infectionImmunocompetent hosts