1995
Isotype-specific Activation of Cystic Fibrosis Transmembrane Conductance Regulator-Chloride Channels by cGMP-dependent Protein Kinase II (∗)
French P, Bijman J, Edixhoven M, Vaandrager A, Scholte B, Lohmann S, Nairn A, de Jonge H. Isotype-specific Activation of Cystic Fibrosis Transmembrane Conductance Regulator-Chloride Channels by cGMP-dependent Protein Kinase II (∗). Journal Of Biological Chemistry 1995, 270: 26626-26631. PMID: 7592887, DOI: 10.1074/jbc.270.44.26626.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCattleCell LineCell MembraneChloride ChannelsCyclic GMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorEnzyme InhibitorsIntestinesIsoenzymesKineticsLungMacromolecular SubstancesMarine ToxinsMembrane PotentialsMicrovilliOxazolesPeptide FragmentsPhosphopeptidesPhosphorylationProtein Phosphatase 1Protein Tyrosine PhosphatasesRatsRecombinant ProteinsSwineTransfectionConceptsProtein kinaseType II cGMP-dependent protein kinaseCGMP-dependent protein kinase IICAMP-dependent protein kinaseCystic fibrosis transmembrane conductance regulator (CFTR) chloride channelCGMP-dependent protein kinaseCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorProtein kinase IINIH 3T3 fibroblastsRat intestinal cell lineRecombinant CFTRCF 2Presence of cGMPProtein phosphatasePresence of ATPCAK activationPhosphatase 1Phosphopeptide mapsCatalytic subunitCalyculin ACatalytic fragmentKinase IIConductance regulator
1994
Regulation of CFTR channel gating
Gadsby D, Nairn A. Regulation of CFTR channel gating. Trends In Biochemical Sciences 1994, 19: 513-518. PMID: 7531880, DOI: 10.1016/0968-0004(94)90141-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChloride ChannelsCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorHumansIon Channel GatingMembrane ProteinsConceptsChannel gatingCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelAMP-dependent protein kinaseCFTR channel gatingReceptor-mediated activationRegulatory domainProtein kinaseATP hydrolysisCFTR channelsCl- channelsEpithelial cellsChannel openingComplex mechanismsCellsRecent advancesKinaseGenesPhosphorylationSerineGatingCFTRMutationsRegulationStemActivationRegulation of the gating of cystic fibrosis transmembrane conductance regulator C1 channels by phosphorylation and ATP hydrolysis.
Hwang T, Nagel G, Nairn A, Gadsby D. Regulation of the gating of cystic fibrosis transmembrane conductance regulator C1 channels by phosphorylation and ATP hydrolysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 4698-4702. PMID: 7515176, PMCID: PMC43855, DOI: 10.1073/pnas.91.11.4698.Peer-Reviewed Original ResearchConceptsCFTR channelsATP hydrolysisPresence of ATPDomains of CFTRCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelProtein kinase AATP analogue 5'Kinase AOpen probabilityAMP-PNPPhosphorylationLow open probabilityCl- channelsATPATP actionIntact cardiac myocytesNucleotidesCFTRSecond siteExcised patchesHigh open probabilityCardiac myocytesChannel closingC1 channelsAnalogue 5Coupling of CFTR Cl− channel gating to an ATP hydrolysis cycle
Baukrowitz T, Hwang T, Nairn A, Gadsby D. Coupling of CFTR Cl− channel gating to an ATP hydrolysis cycle. Neuron 1994, 12: 473-482. PMID: 7512348, DOI: 10.1016/0896-6273(94)90206-2.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorATP hydrolysis cycleHydrolysis cycleCFTR channelsFibrosis transmembrane conductance regulatorProtein kinase ATransmembrane conductance regulatorATP hydrolysisKinase AConductance regulatorNucleoside triphosphatesChannel openingInorganic phosphate analogueATPPhosphate analogueCardiac myocytesInorganic phosphateMean open timeRegulatorHydrolysis productsBeF3Open timeCycleTriphosphateRegulation of CFTR channel gating.
Gadsby D, Hwang T, Baukrowitz T, Nagel G, Horie M, Nairn A. Regulation of CFTR channel gating. The Journal Of Physiological Sciences 1994, 44 Suppl 2: s183-92. PMID: 7752525.Peer-Reviewed Original ResearchConceptsNon-hydrolyzable ATP analog AMP-PNPCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelAMP-PNPCFTR channel gatingProtein kinase A (PKA) phosphorylationATP analogue AMP-PNPAnalogue AMP-PNPCFTR's twoA PhosphorylationATP hydrolysisChannel gatingCl- channelsChannel openingNBDRegulationMultiple sitesPhosphorylationCFTROrthovanadateATPGatingDomain
1993
Cardiac Chloride Channels: Incremental Regulation by Phosphorylation/Dephosphorylationa
GADSBY D, HWANG T, HORIE M, NAGEL G, NAIRN A. Cardiac Chloride Channels: Incremental Regulation by Phosphorylation/Dephosphorylationa. Annals Of The New York Academy Of Sciences 1993, 707: 259-274. PMID: 9137557, DOI: 10.1111/j.1749-6632.1993.tb38057.x.Peer-Reviewed Original ResearchLocalization of the cystic fibrosis transmembrane conductance regulator in human bile duct epithelial cells
Cohn J, Strong T, Picciotto M, Nairn A, Collins F, Fitz J. Localization of the cystic fibrosis transmembrane conductance regulator in human bile duct epithelial cells. Gastroenterology 1993, 105: 1857-1864. PMID: 7504645, DOI: 10.1016/0016-5085(93)91085-v.Peer-Reviewed Original Research
1992
The protein kinase A-regulated cardiac CI− channel resembles the cystic fibrosis transmembrane conductance regulator
Nagel G, Hwang T, Nastiuk K, Nairn A, Gadsbyt D. The protein kinase A-regulated cardiac CI− channel resembles the cystic fibrosis transmembrane conductance regulator. Nature 1992, 360: 81-84. PMID: 1279437, DOI: 10.1038/360081a0.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsBiological Transport, ActiveBlotting, NorthernChloride ChannelsChlorineCystic Fibrosis Transmembrane Conductance RegulatorGuanosine TriphosphateGuinea PigsIn Vitro TechniquesIon Channel GatingMembrane PotentialsMembrane ProteinsMyocardiumPhosphorylationProtein KinasesReceptors, Adrenergic, betaRNAConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorConductance regulatorCyclic AMP-dependent protein kinaseAMP-dependent protein kinasePKA catalytic subunitResult of phosphorylationPhosphorylated channelsCatalytic subunitProtein kinaseSingle-channel conductanceNucleoside triphosphatesPhosphorylationMembrane potentialEpithelial cellsChannel activationRegulatorChannel conductanceCystic fibrosisKinaseCardiac ventricular myocytesSubunitsProteinUnitary current amplitudecGMP-dependent protein kinase regulation of a chloride channel in T84 cells
Lin M, Nairn A, Guggino S. cGMP-dependent protein kinase regulation of a chloride channel in T84 cells. American Journal Of Physiology 1992, 262: c1304-c1312. PMID: 1317106, DOI: 10.1152/ajpcell.1992.262.5.c1304.Peer-Reviewed Original ResearchConceptsProtein kinaseChloride channelsIntestinal epithelial cellsCGMP-dependent protein kinaseProtein kinase regulationApical membraneDependent protein kinaseT84 cellsEpithelial cellsInhibitor of PKGKinase regulationCatalytic subunitCombination of ATPNonhydrolyzable formExcessive fluid secretionIntracellular faceEscherichia coliElevated guanosineSingle-channel recordingsATPKinasePKGLinear current-voltage relationshipCellsChloride secretionRole of GTP-binding proteins in the regulation of mammalian cardiac chloride conductance.
Hwang T, Horie M, Nairn A, Gadsby D. Role of GTP-binding proteins in the regulation of mammalian cardiac chloride conductance. The Journal Of General Physiology 1992, 99: 465-489. PMID: 1375958, PMCID: PMC2219206, DOI: 10.1085/jgp.99.4.465.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenylate Cyclase ToxinAdrenergic beta-AgonistsAnimalsCarbacholCells, CulturedChloride ChannelsChloridesColforsinCyclic AMPGTP-Binding ProteinsGuanosine 5'-O-(3-Thiotriphosphate)Guinea PigsHeartHistamineIon ChannelsIsoproterenolMembrane ProteinsMyocardiumPertussis ToxinPhosphorylationPropranololReceptors, Adrenergic, betaReceptors, MuscarinicTime FactorsVirulence Factors, BordetellaConceptsProtein kinaseNonhydrolyzable GTP analogG proteinsCAMP-dependent protein kinaseG protein turnoverGTP-binding proteinsCl- conductanceAdenylyl cyclase activityCl- current activationGTP analogueMammalian cardiac myocytesGDP beta SSynthetic peptide inhibitorProtein turnoverStimulatory G proteinMammalian modelsPertussis toxinBeta SInhibitory G proteinBasal activationGTPPeptide inhibitorAdenylyl cyclaseCyclase activityProtein
1989
Regulation of Chloride Channels by Protein Kinase C in Normal and Cystic Fibrosis Airway Epithelia
Li M, McCann J, Anderson M, Clancy J, Liedtke C, Nairn A, Greengard P, Welsch M. Regulation of Chloride Channels by Protein Kinase C in Normal and Cystic Fibrosis Airway Epithelia. Science 1989, 244: 1353-1356. PMID: 2472006, DOI: 10.1126/science.2472006.Peer-Reviewed Original ResearchConceptsProtein kinase CChloride channelsKinase CApical membrane chloride channelMembrane chloride channelCystic fibrosis cellsMembrane proteinsCell-free membraneCystic fibrosis airway epitheliaChloride secretionIntact cellsPhorbol esterPhysiological statusDefective regulationAirway epithelial cellsEpithelial cellsCellsRegulationChannel inactivationCystic fibrosisActivationCalcium concentrationLow calcium concentrationsProteinAirway epithelium