2000
Severed Channels Probe Regulation of Gating of Cystic Fibrosis Transmembrane Conductance Regulator by Its Cytoplasmic Domains
Csanády L, Chan K, Seto-Young D, Kopsco D, Nairn A, Gadsby D. Severed Channels Probe Regulation of Gating of Cystic Fibrosis Transmembrane Conductance Regulator by Its Cytoplasmic Domains. The Journal Of General Physiology 2000, 116: 477-500. PMID: 10962022, PMCID: PMC2233695, DOI: 10.1085/jgp.116.3.477.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAdenylyl ImidodiphosphateAnimalsBase SequenceCyclic AMP-Dependent Protein KinasesCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDNA PrimersFemaleHumansIn Vitro TechniquesIon Channel GatingModels, BiologicalMutationOocytesPhosphorylationProtein Structure, TertiaryRecombinant ProteinsXenopusConceptsR domainCFTR channelsPhosphorylated R domainWild-type CFTR channelsCytoplasmic regulatory domainCystic fibrosis transmembrane conductance regulatorNucleotide Binding DomainFibrosis transmembrane conductance regulatorDetailed functional characteristicsWT channelsApparent ATP affinityTransmembrane conductance regulatorCFTR Cl- channelPresence of PKANonhydrolyzable ATP analogue AMPPNPATP analogue AMPPNPATP bindingRegulatory domainCytoplasmic domainWt-CFTRBinding domainsGating eventsConductance regulatorATP affinityFunctional interaction
1998
ATP hydrolysis cycles and the gating of CFTR Cl- channels.
Gadsby D, Dousmanis A, Nairn A. ATP hydrolysis cycles and the gating of CFTR Cl- channels. Acta Physiologica Scandinavica. Supplementum 1998, 643: 247-56. PMID: 9789567.Peer-Reviewed Original ResearchConceptsC-terminal nucleotideCFTR channelsAMP-PNPG proteinsN-terminal nucleotideCentral regulatory domainMore serine residuesProtein kinase ACFTR Cl- channelHydrolysis of ATPATP hydrolysis cycleCl- channelsGating cycleRegulatory domainCytoplasmic domainTight bindingSerine residuesHydrolyse ATPSecond ATPSequence homologyTransport proteinsKinase AOpen conformationAnalogues of ATPFunctional similarity